OMX BLAZE High Speed Super Resolution Imaging System

OMX BLAZE 高速超分辨率成像系统

• 批准号: 8333280
• 负责人: Aaron F Straight
• 金额: $ 110.95万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8333280
• 关键词: Architecture; Area; Bardet-Biedl Syndrome; Biogenesis; Biological Process; Cells; Centromere; Centrosome; Chromosome Segregation; Cilia; Congenital Abnormality; Cystic kidney; DNA Sequence Rearrangement; Development; Disease; Docking; Epithelial Cells; Funding; Genetic Engineering; Genetic Recombination; Glass; Health; Human; Image; Imaging technology; Inherited; Kinetochores; Lateral; Life; Malignant Neoplasms; Meiosis; Microfilaments; Microscopy; Molecular; Neurodegenerative Disorders; Neurons; Obesity; Request for Proposals; Research; Research Personnel; Resolution; Resource Sharing; Science; Scientist; Secretory Vesicles; Speed; Structure; Synapses; System; Tissues; Touch sensation; United States National Institutes of Health; biological research; cell transformation; cellular imaging; design; fluorescence imaging; frontier; gastrulation; imaging modality; light microscopy; neural circuit; neurodevelopment; receptor; smoothened signaling pathway

DESCRIPTION (provided by applicant): Our understanding of fundamental cell biological processes is both driven by and limited by our ability to visualize the organization of structures and molecules within cells and tissues. The development of "super-resolution" fluorescence imaging methods that circumvent the resolution limits of conventional light microscopy, achieving lateral resolution of d100 nm, are transforming cell biological research. Access to super-resolution fluorescence imaging technology is now essential for scientists wishing to push the frontiers of cell biological research. This proposal requests funds to purchase the OMX BLAZE 2D, 3D-SIM fast super-resolution imaging system (Applied Precision, Inc.). This super-resolution system can achieve both lateral and axial resolution at twice the diffraction limit of conventional light microscopy and is capable of multi- channel super-resolution far beyond the cover glass. The fast imaging capability of OMX BLAZE is also designed to overcome speed limitations for 3D live-cell imaging. This advanced imaging system will be a shared resource, located in a well-established, multi-user microscopy facility at Stanford: the Cell Sciences Imaging Facility. The requested OMX BLAZE 3D-SIM imaging system will support NIH funded projects from 14 researchers. These projects investigate a wide range of topics, including: meiotic chromosome segregation (Villeneuve); genetic recombination (Villeneuve); centrosome structure, function and duplication (Stearns); centromere and kinetochore assembly (Straight); biogenesis and function of the primary cilium (Nachury, Rohatgi, Stearns); architecture of neural circuits (Smith); molecular mechanisms underlying epithelial cell rearrangements during gastrulation (Nelson); actin filament assembly and dynamics (Nelson); molecular mechanisms of secretory vesicle docking and fusion (Pfeffer); development of neural synapses (Shen); mechano-electrical transduction in touch receptor neurons (Goodman); recombination and genetic engineering (Porteus); Hedgehog pathway signaling in development and cancer (Chen, Scott, Beachy, Rohatgi). These studies investigate critical functional and structural questions regarding fundamental cell biological processes and cover NIH research areas with implications for diverse aspects of human health and disease, including cancer, birth defects, obesity, kidney cysts (in the inherited Bardet-Biedl Syndrome) and neurodegenerative disease. All these projects require multi-channel super-resolution imaging and simultaneous multi-channel fast imaging; this combination of capabilities is most effectively provided by the requested OMX BLAZE 3D SIM system.

描述（由申请人提供）：我们对基本细胞生物过程的理解既受到我们可视化细胞和组织内结构和分子组织的能力的驱动，又受到我们的限制。 “超分辨率”荧光成像方法的发展突破了传统光学显微镜的分辨率限制，实现了 d100 nm 的横向分辨率，正在改变细胞生物学研究。对于希望推动细胞生物学研究前沿的科学家来说，获得超分辨率荧光成像技术现在至关重要。该提案要求资金购买 OMX BLAZE 2D、3D-SIM 快速超分辨率成像系统（Applied Precision, Inc.）。该超分辨率系统可以实现两倍于传统光学显微镜衍射极限的横向和轴向分辨率，并且能够实现远远超出盖玻片的多通道超分辨率。 OMX BLAZE 的快速成像功能还旨在克服 3D 活细胞成像的速度限制。这种先进的成像系统将成为共享资源，位于斯坦福大学完善的多用户显微镜设施中：细胞科学成像设施。所请求的 OMX BLAZE 3D-SIM 成像系统将支持 NIH 资助的 14 名研究人员的项目。这些项目研究了广泛的主题，包括：减数分裂染色体分离（Villeneuve）；基因重组（维伦纽夫）；中心体结构、功能和复制（Stearns）；着丝粒和着丝粒组装（直）；初级纤毛的生物发生和功能（Nachury、Rohatgi、Stearns）；神经回路的架构（史密斯）；原肠胚形成过程中上皮细胞重排的分子机制（Nelson）；肌动蛋白丝组装和动力学（Nelson）；分泌囊泡对接和融合的分子机制（Pfeffer）；神经突触的发育（Shen）；触觉感受器神经元中的机电转导（Goodman）；重组和基因工程（Porteus）；发育和癌症中的 Hedgehog 通路信号传导（Chen、Scott、Beachy、Rohatgi）。这些研究调查了有关基本细胞生物过程的关键功能和结构问题，涵盖了 NIH 研究领域，对人类健康和疾病的各个方面都有影响，包括癌症、出生缺陷、肥胖、肾囊肿（遗传性巴代-比德尔综合症）和神经退行性疾病。所有这些项目都需要多通道超分辨率成像和同时多通道快速成像；所需的 OMX BLAZE 3D SIM 系统可以最有效地提供这种功能组合。