Functional Optical Coherence Tomography for Monitoring Drug Resistance in Cancer

用于监测癌症耐药性的功能光学相干断层扫描

• 批准号: 8307913
• 负责人: Melissa Caroline Skala
• 金额: $ 23.95万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8307913
• 关键词: Blood Vessels; Cancer Patient; Caring; Cells; Clinical; Combined Modality Therapy; Dose; Drug Formulations; Drug resistance; ERBB2 gene; Environment; Future; Growth; HER2 inhibition; Human; Image; Imaging Techniques; Instruction; Kinetics; Malignant Neoplasms; Metabolic; Methods; Microscope; Microscopy; Molecular; Monitor; Mus; Optical Coherence Tomography; Penetration; Pharmaceutical Preparations; Pharmacodynamics; Pre-Clinical Model; Preclinical Drug Evaluation; Receptor Cell; Receptor Inhibition; Resolution; Schedule; Signal Transduction; Structure; Techniques; Technology; Therapeutic; Translating; Trastuzumab; Work; bevacizumab; cancer therapy; cellular imaging; hemodynamics; imaging modality; in vivo; molecular imaging; novel; pre-clinical; receptor expression; response; success; therapeutic target; tumor; tumor growth

Molecular signaling is a critical therapeutic target in cancer, and molecular therapeutics have achieved some success in treating a variety of cancers. However, our incomplete l^nowledge of pharmacodynamic effects in vivo limits the success of these molecular therapies. We have recently developed a new molecular imaging technique, photothermal optical coherence tomography (OCT), that extends the penetration depth of high resolution molecular Imaging beyond the microscopy limit. The current proposal will further develop photothermal OCT for in vivo imaging of cell receptors, and will combine this new technique with the hemodynamic imaging capabilities of Doppler OCT and spectral microscopy to provide a comprehensive picture of the pharmacodynamic effects of molecular cancer therapies in human cells grown into tumors in the mouse window chamber. The first aim will use a combined spectral / OCT microscope lo quantify changes in metabolic rate and hemodynamics with tumor growth. The second aim will develop photothermal OCT for in vivo imaging of cell receptors, and will quantify the pharmocodynamics of receptor inhibition in the mouse window chamber. The final aim will combine OCT and microscopy to quantify longitudinal changes in receptor expression and hemodyanmic / metabolic response to combination therapies in mouse window chamber tumors. These studies will establish our novel hemodynamic, metabolic and molecular imaging techniques as high resolution, three-dimensional drug screening methods for preclinical applications. This multi-disciplinary environment will provide an excellent opportunity for professional growth and scientific advancement.

分子信号传导是癌症的关键治疗靶点，分子疗法已经取得了一些成果 成功治疗多种癌症。然而，我们对药效学作用的了解并不完全。 体内限制了这些分子疗法的成功。我们最近开发了一种新的分子成像 光热光学相干断层扫描 (OCT) 技术，可扩展高穿透深度 分辨率分子成像超出显微镜极限。目前的提案将进一步发展 用于细胞受体体内成像的光热 OCT，并将这项新技术与 多普勒 OCT 和光谱显微镜的血流动力学成像功能提供全面的 分子癌症疗法对生长成肿瘤的人类细胞的药效学影响图 鼠标窗室。第一个目标将使用组合光谱/OCT 显微镜来量化 代谢率和血流动力学随肿瘤生长的变化。第二个目标是开发光热 OCT 用于细胞受体的体内成像，并将量化细胞受体抑制的药效动力学 鼠标窗室。最终目标将结合 OCT 和显微镜来量化 小鼠窗口中受体表达和对联合疗法的血流动力学/代谢反应 腔室肿瘤。这些研究将建立我们新颖的血流动力学、代谢和分子成像 技术作为临床前应用的高分辨率、三维药物筛选方法。这 多学科环境将为专业成长和科学发展提供绝佳机会 进步。

项目成果

Three-dimensional molecular imaging with photothermal optical coherence tomography.

• DOI: 10.1007/978-1-62703-468-5_7
• 发表时间: 2013
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Skala, Melissa C;Crow, Matthew J;Wax, Adam;Izatt, Joseph A
• 通讯作者: Izatt, Joseph A

In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye.

内源性和外源性对比剂的体内光热光学相干断层扫描。

• DOI: 10.1038/s41598-017-10050-5
• 发表时间: 2017-08-23
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Lapierre-Landry M;Gordon AY;Penn JS;Skala MC
• 通讯作者: Skala MC

In vivo photothermal optical coherence tomography of gold nanorod contrast agents.

• DOI: 10.1364/boe.3.002881
• 发表时间: 2012-11-01
• 期刊: Biomedical optics express
• 影响因子: 3.4
• 作者: Tucker-Schwartz JM;Meyer TA;Patil CA;Duvall CL;Skala MC
• 通讯作者: Skala MC

Dual-modality photothermal optical coherence tomography and magnetic-resonance imaging of carbon nanotubes.

• DOI: 10.1364/ol.37.000872
• 发表时间: 2012-03-01
• 期刊: Optics letters
• 影响因子: 3.6
• 作者: Tucker-Schwartz JM;Hong T;Colvin DC;Xu Y;Skala MC
• 通讯作者: Skala MC

Cross-sectional tracking of particle motion in evaporating drops: flow fields and interfacial accumulation.

• DOI: 10.1021/la400542x
• 发表时间: 2013-05-28
• 期刊: Langmuir : the ACS journal of surfaces and colloids
• 作者: Trantum JR;Eagleton ZE;Patil CA;Tucker-Schwartz JM;Baglia ML;Skala MC;Haselton FR
• 通讯作者: Haselton FR