Treatment of disc degeneration by nano-fullerenes

纳米富勒烯治疗椎间盘退变

• 批准号: 8309470
• 负责人: XUDONG J. LI
• 金额: $ 20.79万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8309470
• 关键词: Animal Experiments; Apoptosis; Apoptotic; Back; Back Pain; Biochemistry; Biological; Cell membrane; Cell-Free System; Cells; Chemical Structure; Chemicals; Chronic; Clinical; Environment; Excision; Exhibits; Extracellular Matrix; Fullerenes; Growth Factor; Half-Life; Human; Hydrogen Peroxide; In Vitro; Inhibition of Apoptosis; Interleukin-1; Interleukins; Intervertebral disc structure; Lead; Low Back Pain; Methods; Modality; Modeling; Mono-S; Nanostructures; Natural regeneration; Needles; Operative Surgical Procedures; Oryctolagus cuniculus; Outcome; Oxidative Stress; Pain; Physicians; Play; Preparation; Prevention; Process; Property; Proteins; Publishing; Puncture procedure; Reactive Oxygen Species; Reporting; Research; Role; Scheme; Science; Scientist; Societies; Solubility; Solutions; Staging; Supplementation; Surgeon; Suspension substance; Suspensions; Symptoms; Techniques; Therapeutic; Tissue Engineering; Tissues; Vertebral column; Yang; aqueous; base; disability; experience; fullerene C60; in vivo; intervertebral disk degeneration; nano; nanoparticle; nanostructured; novel; novel therapeutics; nucleus pulposus; professor; treatment strategy

DESCRIPTION (provided by applicant): Symptomatic intervertebral disc degeneration appears to be one of the most prevalent causes of chronic low back symptoms. Although therapeutic techniques including surgical removal and tissue engineering have been applied/proposed, the treatment of disc degeneration still remains a great challenge to both clinical physicians and basic scientists. Oxidative stress is regarded to play an important role in the early stages of disc degenerative process. However, therapeutic strategies using antioxidative agents have been ignored in the treatment of disc degeneration, and in particular, there are no reports on the effects of fullerene and its derivatives on disc degeneration published. The aim of the project is to characterize the inhibitory effects of aqueous solutions of a specific fullerene C60 derivative, bis- methanophosphonate fullerene (BMPF) on degenerative changes of intervertebral disc in vitro and in vivo based on the hypothesis that they might be very effective in the treatment of disc degeneration. Aqueous nanoparticle suspension will be prepared from fullerene and its derivatives including BMPF and C60, a mono- methanophosphonate fullerene, a fullerol and two carboxyfullerenes. Their protective activities against apoptosis and matrix destruction of cultured human disc cells induced by hydrogen peroxide or IL-1 will be investigated. Their inhibitory effects on disc degeneration will be further evaluated by using a rabbit annulus needle puncture model. The present study involving chemical, materials science and biomedicine will provide useful clues to the therapeutic strategy of disc degeneration and possibly lead to some novel potential candidates for the treatment of disc degeneration.

描述（由申请人提供）：症状性椎间盘退变似乎是慢性腰痛症状的最常见原因之一。尽管包括手术切除和组织工程在内的治疗技术已被应用/提出，但椎间盘退变的治疗仍然是临床医生和基础科学家的巨大挑战。氧化应激被认为在椎间盘退变过程的早期阶段起重要作用。然而，在椎间盘退变的治疗中，使用抗氧化剂的治疗策略一直被忽视，特别是，没有关于富勒烯及其衍生物对椎间盘退变的影响的报道。 该项目的目的是表征特定富勒烯C60衍生物，双甲烷膦酸酯富勒烯（BMPF）的水溶液对椎间盘退行性变化的抑制作用，基于它们可能在椎间盘退变的治疗中非常有效的假设。水性纳米颗粒悬浮液将由富勒烯及其衍生物（包括BMPF和C60）、单甲膦酸酯富勒烯、富勒醇和两种羧基富勒烯制备。将研究它们对过氧化氢或IL-1诱导的培养人椎间盘细胞凋亡和基质破坏的保护活性。将使用兔纤维环针刺模型进一步评价它们对椎间盘退变的抑制作用。目前的研究涉及化学，材料科学和生物医学将提供有用的线索，椎间盘退变的治疗策略，并可能导致一些新的潜在候选人治疗椎间盘退变。

项目成果

Animal models for disc degeneration-an update.

• DOI: 10.14670/hh-11-910
• 发表时间: 2018-06
• 期刊: Histology and histopathology
• 影响因子: 2
• 作者: Jin L;Balian G;Li XJ
• 通讯作者: Li XJ

A novel culture platform for fast proliferation of human annulus fibrosus cells.

一个新型的培养平台，用于快速的人环纤维细胞增殖。

• DOI: 10.1007/s00441-016-2497-4
• 发表时间: 2017-02
• 期刊: Cell and tissue research
• 影响因子: 3.6
• 作者: Xiao L;Ding M;Saadoon O;Vess E;Fernandez A;Zhao P;Jin L;Li X
• 通讯作者: Li X

Antioxidative nanofullerol prevents intervertebral disk degeneration.

• DOI: 10.2147/ijn.s60853
• 发表时间: 2014
• 期刊: International journal of nanomedicine
• 影响因子: 8
• 作者: Yang X;Jin L;Yao L;Shen FH;Shimer AL;Li X
• 通讯作者: Li X

Fullerol nanoparticles suppress inflammatory response and adipogenesis of vertebral bone marrow stromal cells--a potential novel treatment for intervertebral disc degeneration.

• DOI: 10.1016/j.spinee.2013.04.004
• 发表时间: 2013-11
• 期刊: SPINE JOURNAL
• 影响因子: 4.5
• 作者: Liu, Qihai;Jin, Li;Shen, Francis H.;Balian, Gary;Li, Xudong Joshua
• 通讯作者: Li, Xudong Joshua

A New Formyl Peptide Receptor-1 Antagonist Conjugated Fullerene Nanoparticle for Targeted Treatment of Degenerative Disc Diseases.

• DOI: 10.1021/acsami.9b11783
• 发表时间: 2019-09
• 期刊: ACS applied materials & interfaces
• 影响因子: 9.5
• 作者: Li Xiao;Rong Huang;Yi Zhang;Tinghui Li;Jun Dai;Naga Nannapuneni;Timothy R. Chastanet;Matthew Chen;F. Shen;Li Jin;H. Dorn;X. Li