A new hemagglutination method for detection of unexpected antibodies

描述(申请人提供)：拟议研究的长期目标是开发一种新的定量颗粒凝集技术，以提高检测红细胞抗原同种抗体的检测性能。传统的血凝血型检测方法存在灵敏度低、孵育时间长、主观性强、试剂用量大等缺点。正是在这种背景下，我们提议开发一种新的技术，它摆脱了传统血凝试验的上述限制。所提出的免疫分析是一种使用廉价仪器的灵敏和快速的方法。值得注意的是，该方法为增强和检测不同来源的颗粒(包括胶乳、二氧化硅微粒和生物细胞)的凝集提供了一种通用的方法。 公共卫生相关性：该方法的发展将使人们能够获得定量结果，提高颗粒和红细胞凝集分析的灵敏度，并在不影响每次检测成本的情况下减少分析时间。这些研究的成功完成将为获得许可技术和制造商业产品的大量商业机会提供所需的所有技术。

DESCRIPTION (provided by applicant): The long-term goal of the proposed research is to develop a novel quantitative particle agglutination technology for improving the performance of assays for detection of allo-antibodies to red blood cell antigens. The conventional hemagglutination blood typing assays have serious drawbacks such as limited sensitivity, long time of incubation, subjectivity and large amount of reagents. It is in this context that we propos to develop a new technology, which is free from the aforementioned limitations of the conventional hemagglutination assays. The proposed immunoassay is a sensitive and rapid method that utilizes inexpensive instrumentation. Significantly, the method presents a general approach for enhancement and detection of agglutination of particles of different origin, including latex, silica microparticles, and biological cells. PUBLIC HEALTH RELEVANCE: The development of this method will allow one to obtain quantitative results, increase the sensitivity of particle and red cell agglutination assays, and reduce time of analysis without compromising the cost per test. Successful completion of these studies will make available all of the technology needed for a substantial business opportunity to license the technology and manufacture commercial products.