A new hemagglutination method for blood grouping

描述(由申请人提供)：拟议研究的长期目标是开发一种新的定量颗粒凝集技术，以提高血型分析的性能。传统的血凝血型检测方法存在灵敏度低、孵育时间长、主观性强、试剂用量大等缺点。正是在这种背景下，我们建议开发一种新的技术，它摆脱了传统血凝分析的上述限制。所提出的免疫分析是一种使用廉价仪器的灵敏和快速的方法。第二阶段的具体目标是：1)进一步探讨影响执行情况的所有变数 新的血型检测分析和优化了检测的性能；2)设计和组装了一台同时进行多个血型检测的设备的�转型。�分型的目的是在最终试生产样机中实施之前对系统的每一个技术要素的设计进行测试；3)验证用于确定血型(包括弱D血型和C、c、E、e和K抗原)和预期抗体(反向分型)的检测方法；对每种检测方法检验具有统计意义的大量临床样本，并进行统计分析，证明获得的结果的重复性；比较传统检测方法和新开发的检测方法的性能。值得注意的是，新方法为增强和检测不同来源的粒子的凝集作用提供了一种通用的方法，包括胶乳、硅微粒子和生物细胞。

DESCRIPTION (provided by applicant): The long-term goal of the proposed research is to develop a novel quantitative particle agglutination technology for improving the performance of blood typing assays. The conventional hemagglutination blood typing assays have serious drawbacks such as limited sensitivity, long time of incubation, subjectivity and large amount of reagents. It is in this context that we propose to develop a new technology, which is free from the aforementioned limitations of the conventional hemagglutination assays. The proposed immunoassay is a sensitive and rapid method that utilizes inexpensive instrumentation. The Specific Aims of Phase II are: 1) To further explore all variables affecting the performance of the new blood typing detection assays and optimize the performance of the assay; 2) To design and assemble an �rototype of the device for performing of several blood typing assays simultaneously. The �rototype is intended to test the design of every technical element of the system before it is implemented in the final pre- production prototype; 3) To validate the detection assays for determination of blood groups (including weak D group and C, c, E, e and K antigens) and expected antibodies (reverse typing); To examine a statistically significant number of clinical samples for each assay, and carry out statistical analysis and demonstrate reproducibility of the obtained results; To compare performance of the conventional and newly developed assays. Significantly, the new method presents a general approach for enhancement and detection of agglutination of particles of different origin, including latex, silic microparticles, and biological cells.