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Ultrasound monitored HIV rapid test

超声监测 HIV 快速检测

基本信息

批准号：
6747159
负责人：
Simon Bystryak
金额：
$ 12.76万
依托单位：
ALLIED INNOVATIVE SYSTEMS, LLC
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-03-01 至 2005-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6747159
关键词：
AIDS /HIV test agglutination reaction bioengineering /biomedical engineering biomedical equipment development diagnosis design /evaluation diagnostic tests enzyme linked immunosorbent assay human immunodeficiency virus immunologic assay /test method development rapid diagnosis ultrasound

项目摘要

DESCRIPTION (provided by applicant): The objective of this proposal is to demonstrate the feasibility of a novel method for detection of human immunodeficiency virus (HIV) antibody in the body fluids. The method is based on the use of ultrasound to significantly increase the sensitivity and reduce time of analysis of conventional particle agglutination immunoassay. Ultrasound waves are used for both accelerating and monitoring of the agglutination process. Proposed technology exploits the sequence of physical phenomena in the ultrasonic standing wave field including (a) accumulation of particle aggregates in the nodes of the standing acoustic wave and acceleration of the agglutination process; (b) generation of microbubbles in the nodes of the standing acoustic wave, where the latex particle aggregates serve as microbubble nucleation centers; (c) the growth of the microbubbles in the ultrasonic field due to rectified diffusion of the dissolved gas from the liquid into the microbubble; and (d) the following cavitation activity which provides gigantic amplification of the "signal" indicating the formation of the particle aggregate due to antigen-antibody reaction. The immunoassay we propose to develop will be implemented in a simple, portable and inexpensive device based on the novel patented cylindrical resonator technology. Significantly, the method can be easily adapted to detect any target molecule for which antibodies can be raised. Preliminary studies confirmed feasibility of the major innovative components of the proposed technology and it is shown that the new method has significant commercial and scientific potential.

描述（由申请人提供）：本提案的目的是证明一种检测体液中人类免疫缺陷病毒（HIV）抗体的新方法的可行性。该方法基于超声波的使用，显着提高了传统颗粒凝集免疫分析的灵敏度并减少了分析时间。超声波用于加速和监测凝集过程。所提出的技术利用了超声波驻波场中的一系列物理现象，包括（a）驻声波节点中颗粒聚集体的积累和凝集过程的加速； (b) 在驻声波的节点中产生微泡，其中乳胶颗粒聚集体充当微泡成核中心； (c)由于溶解气体从液体到微泡的整流扩散而在超声波场中微泡的生长； (d)随后的空化活性，其提供了指示由于抗原-抗体反应而形成颗粒聚集体的“信号”的巨大放大。我们建议开发的免疫测定将在基于新型专利圆柱形谐振器技术的简单、便携式且廉价的设备中实施。值得注意的是，该方法可以很容易地适用于检测任何可以产生抗体的靶分子。初步研究证实了所提出技术的主要创新部分的可行性，并表明该新方法具有巨大的商业和科学潜力。