Molecular Imaging Reporter

分子影像记者

• 批准号: 8195499
• 负责人: David Piwnica-Worms
• 金额: $ 15.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8195499
• 关键词: Adverse effects; Caloric Restriction; Cancer Patient; Cells; Cessation of life; Chemoprotective Agent; Clinical Trials; Clinical effectiveness; DNA Damage; Development; Dose; Effectiveness; Engineering; Etoposide; Fasting; Fatigue; Firefly Luciferases; Food deprivation (experimental); Genotoxic Stress; Growth; Heart; High Dose Chemotherapy; Hypothalamic structure; Instruction; Intervention; Ionizing radiation; Knockout Mice; Literature; Malignant neoplasm of prostate; Mediating; Metabolic; Metabolism; Molecular; Mus; Mutation; Neurons; Neutropenia; Normal Cell; Normal tissue morphology; Organ; PTEN gene; Pathway interactions; Publishing; Radiation therapy; Radio; Reporter; Reporting; Role; Signal Pathway; Starvation; Testing; Therapeutic; Time; Topoisomerase; Toxic effect; Universities; Washington; Xenograft procedure; cancer cell; cancer type; chemotherapy; dietary restriction; effective intervention; feeding; gastrointestinal; inhibitor/antagonist; irinotecan; killings; molecular imaging; mouse model; neoplastic cell; novel; oncoprotein p21; preconditioning; programs; promoter; research study; response; transcription factor; tumor

The Molecular Imaging Reporter Core (IVIIRC) is a central facility (See GENERAL RESOURCES AND ENVIR0NI\/1ENT). David Piwnica-Worms, PSO PI, also serves as Reporter Core Director. Julie Prior, B.S., is the Senior Core Manager. Ms. Prior is a seasoned Medical Research Lab Manager with 15 years experience in biomedical research/molecular imaging projects, and has been a part ofthe PSO ICMIC program here at Washington University since its inception. She has extensive experience with cell biology, tracer assays, biochemistry and all aspects of bioluminescence animal and cellular imaging as well as lab management. She is the primary technical investigator and directs two FTE's engaged in active experimentation, cloning of MIRC reporter genes, engineering constructs, and managing a catalog of vector systems for general use in reporting the regulation of genes of interest for scientists involved with ICMIC activities. Julie also actively participates in discovery research projects. The MIRC not only develops and generates new reporter constructs, cell lines and geneticallyengineered animals, but also provides expertise, materials and collaborative assistance for design and execution of the biological aspects of molecular imaging. The MIRC serves investigators with a wide range of resources and experience in niolecular biology, mammalian cell culture, and small animal experimentation. One of the most important service activities of this core is dissemination of our newly developed molecular imaging reagents and geneticallyencoded reporters to investigators within our institution, to other PSO program sites and to cancer biology investigators throughout the world (Figure 1). The MIRC has been and continues to be one of our most productive and comprehensive cores, impacting a broad range of programs throughout the world through our discovery research activities.

分子成像报告器核心(IVIIRC)是一个中央设施(请参阅一般参考资料和 环境(ENVIR0NI\/1ENT)。PSO Pi David Piwnica-Worms兼任记者核心总监。朱莉·普赖尔 B.S.是高级核心经理。普赖尔女士是一位有15年经验的医学研究实验室经理 有生物医学研究/分子成像项目的经验，并已成为PSO ICMIC计划的一部分 华盛顿大学从一开始就在这里。她在细胞生物学、示踪剂方面有丰富的经验 分析、生物化学和生物发光的方方面面动物和细胞成像以及实验室 管理层。她是主要的技术调查员，并指导了两个从事活动的全职人员 实验、MIRC报告基因的克隆、工程构建和管理载体目录 通用系统，供参与研究的科学家报告感兴趣的基因的调节 ICMIC活动。朱莉还积极参与发现研究项目。 MIRC不仅开发和产生新的报告结构、细胞系和基因工程 动物，但也为设计提供专业知识、材料和协作协助 以及执行分子成像的生物学方面。MIRC为调查人员提供服务 在分子生物学、哺乳动物细胞培养和 小动物实验。这一核心最重要的服务活动之一 我们新开发的分子成像试剂的传播和遗传编码 记者采访我们机构内的研究人员、其他PSO项目站点以及世界各地的癌症生物学研究人员(图1)。MIRC一直是，并将继续是我们最多产和最全面的核心之一，通过我们的探索研究活动影响到世界各地的广泛项目。