Molecular Imaging Reporter

分子影像记者

• 批准号: 8195499
• 负责人: David Piwnica-Worms
• 金额: $ 15.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8195499
• 关键词: Adverse effects; Caloric Restriction; Cancer Patient; Cells; Cessation of life; Chemoprotective Agent; Clinical Trials; Clinical effectiveness; DNA Damage; Development; Dose; Effectiveness; Engineering; Etoposide; Fasting; Fatigue; Firefly Luciferases; Food deprivation (experimental); Genotoxic Stress; Growth; Heart; High Dose Chemotherapy; Hypothalamic structure; Instruction; Intervention; Ionizing radiation; Knockout Mice; Literature; Malignant neoplasm of prostate; Mediating; Metabolic; Metabolism; Molecular; Mus; Mutation; Neurons; Neutropenia; Normal Cell; Normal tissue morphology; Organ; PTEN gene; Pathway interactions; Publishing; Radiation therapy; Radio; Reporter; Reporting; Role; Signal Pathway; Starvation; Testing; Therapeutic; Time; Topoisomerase; Toxic effect; Universities; Washington; Xenograft procedure; cancer cell; cancer type; chemotherapy; dietary restriction; effective intervention; feeding; gastrointestinal; inhibitor/antagonist; irinotecan; killings; molecular imaging; mouse model; neoplastic cell; novel; oncoprotein p21; preconditioning; programs; promoter; research study; response; transcription factor; tumor

The Molecular Imaging Reporter Core (IVIIRC) is a central facility (See GENERAL RESOURCES AND ENVIR0NI\/1ENT). David Piwnica-Worms, PSO PI, also serves as Reporter Core Director. Julie Prior, B.S., is the Senior Core Manager. Ms. Prior is a seasoned Medical Research Lab Manager with 15 years experience in biomedical research/molecular imaging projects, and has been a part ofthe PSO ICMIC program here at Washington University since its inception. She has extensive experience with cell biology, tracer assays, biochemistry and all aspects of bioluminescence animal and cellular imaging as well as lab management. She is the primary technical investigator and directs two FTE's engaged in active experimentation, cloning of MIRC reporter genes, engineering constructs, and managing a catalog of vector systems for general use in reporting the regulation of genes of interest for scientists involved with ICMIC activities. Julie also actively participates in discovery research projects. The MIRC not only develops and generates new reporter constructs, cell lines and geneticallyengineered animals, but also provides expertise, materials and collaborative assistance for design and execution of the biological aspects of molecular imaging. The MIRC serves investigators with a wide range of resources and experience in niolecular biology, mammalian cell culture, and small animal experimentation. One of the most important service activities of this core is dissemination of our newly developed molecular imaging reagents and geneticallyencoded reporters to investigators within our institution, to other PSO program sites and to cancer biology investigators throughout the world (Figure 1). The MIRC has been and continues to be one of our most productive and comprehensive cores, impacting a broad range of programs throughout the world through our discovery research activities.

分子成像报告核心（IVIIRC）是一个中央设施（见一般资源和 （C/CN0NI/1ENT）。大卫Piwnica-Worms，PSO PI，也担任记者核心主任。朱莉·普赖尔 学士学位，是高级核心经理。Prior女士是一位经验丰富的医学研究实验室经理， 在生物医学研究/分子成像项目方面的经验，并一直是PSO ICMIC计划的一部分 就一直在华盛顿大学工作。她在细胞生物学，示踪剂， 分析、生物化学和生物发光动物和细胞成像以及实验室 管理她是主要的技术调查员，并指导两名全职员工积极参与 实验、MIRC报告基因的克隆、工程构建体和载体目录的管理 用于报告科学家感兴趣的基因调控的通用系统， ICMIC的活动。朱莉还积极参与发现研究项目。 MIRC不仅开发和产生新的报告结构，细胞系和基因工程 动物，但也提供专业知识，材料和合作援助的设计 和执行分子成像的生物学方面。MIRC为调查人员提供服务 在分子生物学，哺乳动物细胞培养， 小动物实验其中最重要的服务活动的核心 是传播我们新开发的分子成像试剂和基因编码的 报告者向我们机构内的研究者、其他PSO项目地点和世界各地的癌症生物学研究者报告（图1）。MIRC一直是并将继续是我们最具生产力和综合性的核心之一，通过我们的发现研究活动影响着世界各地的广泛项目。