structural biology

结构生物学

• 批准号: 8486521
• 负责人: Alfonso Mondragon
• 金额: $ 19.62万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-06 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8486521
• 关键词: Area; Biochemistry; Biological; Biological Sciences; Biophysics; Cancer Center; Cellular biology; Charge; Chemicals; Chemistry; Chicago; Communities; Comprehensive Cancer Center; Computational Biology; Crystallization; Crystallography; Data Collection; Development; Drug Design; Equipment; Funding; Generations; Growth; Imagery; Individual; Laboratories; Malignant Neoplasms; Modeling; Molecular; Molecular Biology; Molecular Structure; Nature; Pharmacology; Phase; Photons; Physics; Proteins; Reader; Research; Research Personnel; Resources; Robot; Roentgen Rays; Science; Source; Structural Biologist; Structure; Techniques; Time; Universities; Work; X ray diffraction analysis; X-Ray Diffraction; base; beamline; cooking; cost effective; design; drug discovery; falls; instrument; instrumentation; interest; light scattering; macromolecule; medical schools; member; programs; protein structure; research study; structural biology; synchrotron radiation

The Structural Biology Facility provides: a) resources required for crystallographic structure determination, refinement and analysis, b) molecular graphics and computational support for structural biology, c) molecular graphics and computational support for structure-based drug discovery and d) highly specialized resources for macromolecular characterization related to structural biology. The Facility is essential for the research programs of investigators of the Cancer Center who are studying the relationship between macromolecular structure and function or who are using macromolecular structure as the starting point for structure-based drug design. It is a unique resource at Northwestern University that capitalizes on the extensive expertise of a large group of users and the unique access to the synchrotron radiation X-ray source at Argonne National Laboratories. It also serves to nucleate the development of a local community with expertise in structural and computational biology. Such expertise will increasingly be called upon as the structures of more cancerrelated proteins become available. The Structural Biology Facility is, as are its investigators, located on both campuses of Northwestern University, based in the Department of Biochemistry, Molecular Biology and Cell Biology on the Evanston campus, in the Department of Molecular Pharmacology and Biological Chemistry at the Medical School campus, and also at the LS-CAT sector of Advanced Photon Source. Dr. Alfonso Mondragon, a structural biologist at the Evanston Campus, directs the Facility. The Facility consists of four major components: 1) an outstation at the APS that is devoted to state-of-the-art macromolecular crystallography, 2) X-ray diffraction facilities at the Chicago and Evanston campuses to support preliminary and more routine crystallographic experiments, 3) computational facilities at both campuses to support structural determination calculations, both NMR and crystallographic, computational drug-design, and modeling efforts, including advanced graphical visualization and manipulation of models, and 4) a relatively new component which provides automated facilities for setting up and visualizing crystallization experiments and for characterization of macromolecules for structural studies. The distributed nature of the facility reflects the means by which the data collection, computational, molecular visualization, and other scientific resources are networked, and thus integrated, for the structural biology research community at Northwestern.

结构生物学设施提供：a)确定晶体结构所需的资源， 改进和分析，b)分子图形和结构生物学的计算支持，c)分子 图形和计算支持基于结构的药物发现和d)高度专业化的资源 用于与结构生物学相关的大分子表征。该设施对研究是必不可少的 癌症中心研究人员正在研究大分子之间关系的程序 结构和功能或谁是使用大分子结构为起点的结构为基础 药物设计。这是西北大学的一项独特资源，它利用了 阿贡国家实验室的一大批用户和对同步辐射X射线源的独特访问 实验室。它还有助于促进当地社区的发展，使其具备结构和技术方面的专门知识 计算生物学。这种专业知识将越来越多地被称为与癌症相关的结构 蛋白质变得可用。 结构生物学设施和它的研究人员一样，都位于西北大学的两个校区 总部设在埃文斯顿大学生物化学、分子生物学和细胞生物学系 医学院分子药理和生物化学系校园内 也在高级光子源的LS-CAT部分。阿方索·蒙塔龙博士，一位结构设计师 埃文斯顿校区的生物学家，该设施的负责人。该机制由四个主要组成部分组成：1)和 APS的分支机构，致力于最先进的大分子结晶学，2)X射线衍射 芝加哥和埃文斯顿校区的设施，以支持初步和更常规的结晶学 实验，3)两个校区的计算设施，以支持结构确定计算， 核磁共振和结晶学、计算药物设计和建模工作，包括高级 模型的图形可视化和操作，以及4)一个相对较新的组件，它提供了 用于建立和可视化结晶实验以及表征的自动化设施 用于结构研究的大分子。该设施的分布式性质反映了 数据收集、计算、分子可视化和其他科学资源是网络化的， 对于西北大学的结构生物学研究社区来说，这样做是整合的。