Raman Flow Cytometry for Diagnostics and Drug Delivery

用于诊断和药物输送的拉曼流式细胞术

• 批准号: 8616667
• 负责人: JOHN P NOLAN
• 金额: $ 46.53万
• 依托单位: LA JOLLA BIOENGINEERING INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2014-07-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8616667
• 关键词: Address; Affinity; Antibodies; Benchmarking; Biomedical Engineering; Cell surface; Cells; Cellular biology; Complex; Custom; Detection; Development; Diagnosis; Diagnostic; Disease; Drug Delivery Systems; Exhibits; Flow Cytometry; Fluorescence; Generations; Goals; Grant; Individual; Lasers; Light; Measurement; Measures; Molecular; Molecular Analysis; Molecular Biology; Nucleic Acids; Performance; Phase; Population; Property; Raman Spectrum Analysis; Reagent; Reporter; Research; Resolution; Sensitivity and Specificity; Signal Pathway; Signal Transduction; Sorting - Cell Movement; Source; Spectrum Analysis; Speed; Surface; System; Testing; Time; Width; Work; base; design; detector; fluorophore; improved; instrument; nanometrology; nanoparticle; novel; particle; prevent; public health relevance; research study; success; theories; tool

DESCRIPTION (provided by applicant): Project Summary The goal of this Bioengineering Research Partnership application is pursue the next phase of development and application of Raman flow cytometry. In the initial project period, we designed and built the first Raman flow cytometers and demonstrated that they have the analysis speed and sensitivity for a wide range of applications. In the second project period, we will develop a Raman-enhanced flow cytometer with both Raman and fluorescence measurement capabilities, develop a new generation of uniform SERS nanotags, and use these in multiparameter analysis of cell signaling pathways. The result of this work will be a significant new tool for the highly multiparameter analysis of cell systems to help understand, diagnose, and prevent disease.

描述（由申请人提供）：项目摘要此生物工程研究伙伴关系应用程序的目标是追求拉曼流式细胞仪的开发和应用的下一阶段。在最初的项目期间，我们设计并构建了第一个拉曼流式细胞仪，并证明它们对广泛应用具有分析速度和灵敏度。在第二个项目期间，我们将开发具有拉曼和荧光测量能力的拉曼增强流式细胞仪，开发新一代均匀的SERS纳米塔，并将其用于细胞信号通路的多参数分析。这项工作的结果将是对细胞系统进行高度多参数分析的重要新工具，以帮助理解，诊断和预防疾病。