Cell Biology and Histology

细胞生物学和组织学核心（CBHC）是本PPG的关键资源，将由所有 项目CBHC将为项目提供以下服务： 1.心脏祖细胞的制备：心脏祖细胞的分离、表征和储存 野生型和转基因小鼠， 2.制备稳定的CPC慢病毒表达系， 3.免疫组织病理学：为所有项目准备组织切片，组织病理学评估 大体和显微心肌结构，心脏切片中CPC的荧光显微成像， 血管分析。 CBHC将位于加州大学圣地亚哥分校。CBHC将容纳和维护 设备，并为项目提供训练有素的工作人员。它将为这四个项目提供一个 各种各样的测定和分析，包括CPC分离、分选和表征，CPC的测定 功能和体内获得的心脏样品的组织病理学。CBHC服务还将包括隔离， 用于项目的来自基因工程小鼠的CPC的维持表征。CBHC还将 扩展和表征Sussman博士分离的人CPC，供其他项目使用。CBHC将 确保所有四个项目都将使用以相同方式隔离、扩展和分类的CPC。这 一致性对于综合和比较不同国家和地区的结果极为重要。 项目CBHC还将协助所有项目准备组织学切片， 组织病理学评估大体和微观心肌结构，包括纤维化程度， 室大小和肌细胞横截面积的定量。它还将分析心脏切片， 通过TUNEL和抗deaved caspase-3免疫荧光染色检测凋亡细胞的存在，以及 通过显微镜评估心脏细胞结构和信号分子定位， 小鼠心脏切片的免疫荧光。最后，CBHC将染色心脏切片以检测c-kit 阳性的CPC加上分化和增殖的标志物，并制备冠状动脉血管铸型。均匀 来自心脏的组织切片的制备和分析对于一致和 四个项目之间的比较结果。 相关性（参见说明）; 当组织缺氧时，心肌细胞死亡，如心肌梗塞（MI;心脏 攻击）。在这里，我们分离祖细胞，我们相信可以取代这些垂死的细胞，并确定如何 让他们更好地治愈心脏我们还检查了心脏内这些细胞的反应， 它们对MI后心脏损伤的影响。

The Cell Biology and Histology Core (CBHC) is a critical resource to this PPG and will be used by all Projects. The CBHC will provide the projects with the following services: 1. preparation of cardiac progenitor cells (CPCs): isolation, characterization, and storage of CPCs from wild type and genetically modified mice, 2. preparation of stable lentiviral expressing lines of CPCs, 3. immunohistopathology: Preparation of histological sections for all projects, histopathology to assess gross and microscopic myocardial structure, fluorescent microscopic imaging of CPCs in heart section, analysis of vasculature. The CBHC will be located at the University of California, San Diego. The CBHC will house and maintain the equipment and provide fully trained staff for the Projects. It will provide the four projects with access to a wide variety of assays and analyses, including CPC isolation, sorting and characterization, assays of CPC function, and histopathology of heart samples obtained in vivo. CBHC services will also include isolation, maintenance characterization of CPCs from genetically engineered mice for the projects. CBHC will also expand and characterize the human CPCs isolated by Dr. Sussman for use by other projects. CBHC will ensure that all four projects will use CPCs that have been isolated, expanded and sorted the same way. This consistency is extremely important for the integration and comparison of results between the projects. The CBHC will also assist all the Projects with preparation of histological sections and histopathology to assess gross and microscopic myocardial structure, including the extent of fibrosis, quantification of chamber size and myocyte cross sectional area. It will also analyze heart sections for presence of apoptotic cells by TUNEL and anti-deaved caspase-3 immunofluorescence staining, and provide microscopic assessment of cardiac cell structure and signaling molecule localization via immunofluorescence of mouse heart sections. Finally, the CBHC will stain heart sections to detect c-kit positive CPCs plus markers of differentiation and proliferation, and prepare coronary vascular casts. Uniform preparation and analysis of histological sections from hearts are also important for consistent and comparable results between the four projects. RELEVANCE (See instructions); Heart muscle cells die when the tissue lacks oxygen, as occurs with a myocardial infarction (Ml; heart attack). Here we isolate progenitor cells that we believe could replace these dying cells and determine how to make them better able to heal the heart. We also examine the response of these cells within the heart and their effects on cardiac damage following Ml.