High Throughput Data Collection Software for Multiplexed TEM Samples

适用于多重 TEM 样品的高通量数据采集软件

• 批准号: 8712794
• 负责人: CLINTON S POTTER
• 金额: $ 22.32万
• 依托单位: NANOIMAGING SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-20 至 2015-06-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8712794
• 关键词: Address; Adoption; Antibodies; Architecture; Automation; Behavior; Benchmarking; Biodistribution; Biological; Biological Models; Complement; Computer software; Data; Data Collection; Data Set; Detection; Development; Documentation; Drug Delivery Systems; Ensure; Environment; Evaluation; Future; Generations; Goals; Health; Hour; Image; Link; Location; Measurement; Methods; Microscope; Monitor; Nanotechnology; Online Systems; Performance; Pharmacologic Substance; Phase; Process; Property; Research Personnel; Resolution; Running; Safety; Sampling; Shapes; Specific qualifier value; Specimen; Stress; System; Techniques; Technology; Testing; Therapeutic; Time; Toxic effect; Transmission Electron Microscopy; Vaccines; Virus; base; biophysical techniques; cost; data acquisition; design; image processing; improved; nanoparticle; nanorod; physical property; predictive modeling; prototype; public health relevance; research and development; screening; software development; therapeutic vaccine; three dimensional structure; tool

DESCRIPTION (provided by applicant): Transmission electron microscopy is a valuable technique for characterizing biological therapeutics, vaccines, drug delivery vehicles, and nanoparticles. There is considerable evidence that links the physical properties of these samples to their bio-distribution, safety, and efficacy. TEM imaging allows direct observation and quantification of these parameters, which include size distributions, shape distributions, concentrations, aggregation states, and 2D and 3D structure determinations. This wealth of information is especially useful when researchers must troubleshoot problems of unknown origin. As bio-therapeutics and nanoparticles increase in size and complexity, TEM is becoming increasingly important as an orthogonal complement to other biophysical methods. Despite the obvious utility of TEM as a direct technique for describing the physicochemical properties of samples in a variety of relevant biological environments, the cost per sample is a significant barrier to the method's widespread adoption, particularly for routine screening of samples. To address this, we will develop software focused on high-throughput data acquisition of multiple samples applied to a single grid substrate. Our long-term goal is to lower the cost per sample for TEM by at least an order of magnitude, and thus establish TEM as an affordable and ubiquitous technique for commercial R&D and testing.

描述（由申请人提供）：透射电子显微镜是表征生物治疗剂、疫苗、药物递送载体和纳米颗粒的有价值的技术。有大量证据表明，这些样品的物理性质与其生物分布、安全性和有效性有关。TEM成像允许直接观察和量化这些参数，包括尺寸分布、形状分布、浓度、聚集状态以及2D和3D结构测定。当研究人员必须解决未知来源的问题时，这些丰富的信息尤其有用。随着生物治疗和纳米颗粒的尺寸和复杂性的增加，TEM作为其他生物物理方法的正交补充变得越来越重要。尽管TEM作为描述样品在各种相关生物环境中的物理化学性质的直接技术具有明显的实用性，但每个样品的成本是该方法广泛采用的重大障碍，特别是对于样品的常规筛选。为了解决这一问题，我们将开发专注于应用于单个网格基板的多个样品的高通量数据采集的软件。我们的长期目标是将TEM的每个样品的成本降低至少一个数量级，从而将TEM建立为商业研发和测试的负担得起且无处不在的技术。