HIGH SCHOOL MARINE PHAGE PROJECT

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 作为为期8周的高中生暑期实习计划的一部分，我们开发了一个 研究项目，实习生从几个地点收集海水样本， 分析TEM图像以确定是否存在杂质。该计划的制定是为了 实习生半独立地决定研究计划。 一个简单的协议是 然后进行样品制备，将过滤后的海水离心分离到带有碳的网格上， 电影然后使用Leginon和TEM对网格成像，然后将图像进行透射电子显微镜（TEM）。 分析了是否存在过氧化氢。 实习生写一份报告并做一个演示。 实习生与NRAMM的工作人员密切合作，并在夏季结束时熟悉 科学研究环境。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. As part of an 8 week summer intern program for high school students, we developed a research program where the intern collects sea water samples from several sites and then analyzes TEM images for the presence of phages. The program has been developed so that the intern is semi-independent in determining the research plan. A simple protocol is followed for sample preparation that centrifuges filtered sea water onto a grid with a carbon film. The grids are then imaged using Leginon and the TEM and the images are then analyzed for the presence of phages. The intern writes a report and gives a presentation. The intern works closely with NRAMM staff and by the end of the summer becomes familiar with the scientific research envirnonment.