HIGH SCHOOL MARINE PHAGE PROJECT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 作为为期8周的高中生暑期实习生计划的一部分，我们开发了一个研究计划，实习生从几个地点收集海水样本，然后分析透射电子显微镜图像中是否存在噬菌体。该计划已经制定，实习生在决定研究计划时是半独立的。一种简单的样品制备方案是将过滤后的海水离心到带有碳膜的栅格上。然后使用莱格农和透射电子显微镜对网格进行成像，然后对图像进行分析以确定是否存在噬菌体。实习生写了一份报告并做了演示。实习生与NRAMM的工作人员密切合作，并在夏末熟悉了科学研究环境。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of an 8 week summer intern program for high school students, we developed a research program where the intern collects sea water samples from several sites and then analyzes TEM images for the presence of phages. The program has been developed so that the intern is semi-independent in determining the research plan. A simple protocol is followed for sample preparation that centrifuges filtered sea water onto a grid with a carbon film. The grids are then imaged using Leginon and the TEM and the images are then analyzed for the presence of phages. The intern writes a report and gives a presentation. The intern works closely with NRAMM staff and by the end of the summer becomes familiar with the scientific research envirnonment.