HIGH SCHOOL MARINE PHAGE PROJECT

高中海洋噬菌体项目

• 批准号: 7956437
• 负责人: CLINTON S POTTER
• 金额: $ 0.65万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956437
• 关键词: Bacteriophages; Carbon; Computer Retrieval of Information on Scientific Projects Database; Film; Funding; Grant; Image; Institution; Marines; Microscopy; Molecular; Preparation; Protocols documentation; Reporting; Research; Research Personnel; Resources; Sampling; Seawater; Site; Source; Students; United States National Institutes of Health; Work; Writing; high school; programs; water sampling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of an 8 week summer intern program for high school students, we developed a research program where the intern collects sea water samples from several sites and then analyzes TEM images for the presence of phages. The program has been developed so that the intern is semi-independent in determining the research plan. A simple protocol is followed for sample preparation that centrifuges filtered sea water onto a grid with a carbon film. The grids are then imaged using Leginon and the TEM and the images are then analyzed for the presence of phages. The intern writes a report and gives a presentation. The intern works closely with NRAMM staff and by the end of the summer becomes familiar with the scientific research envirnonment.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 作为为期8周的高中生暑期实习计划的一部分，我们开发了一个研究项目，实习生从几个地点收集海水样本，然后分析TEM图像中是否存在细菌。该计划已经开发，使实习生在确定研究计划是半独立的。 样品制备遵循一个简单的协议，即将过滤后的海水离心分离到带有碳膜的网格上。然后使用Leginon和TEM对网格进行成像，然后分析图像中是否存在杂质。 实习生写一份报告并做一个演示。实习生与NRAMM工作人员密切合作，并在夏季结束时熟悉科学研究环境。