Animal Models Core

斯克里普斯研究院酒精研究的动物模型/生化测量核心 中心(TSRI-ARC)将提供各种行为和生物分析服务，以满足特定需求 对整个中心的影响。核心的第一个目标是为过度饮酒或有 间歇性酒精饮酒(IAE)模型或慢性饮酒过量饮酒史 对TSRI-ARC和自由研究中心的乙醇诱导依赖(CEID)模型(特定目标1)。 此外，核心还将监督设备和程序的所有变化以及对 目前的动物模型，以确保标准化程序在所有实验室使用。第二 核心的目标是执行生化测量，如血液酒精、皮质醇/皮质酮和 ACTH水平以及脑氨基酸和内源性大麻素含量，以支持所有与中心相关的项目 建立最先进的技术和高效、用户友好的网站来调度服务、监控 进展和接收数据(具体目标2)。最后，核心的最终目标是进一步描述 过度饮酒的动物模型将被TSRI-ARC利用，包括探索 过度饮酒对依赖转化的影响及过度饮酒对脑应激的影响 系统通过表征酗酒史(IAE模型)对大脑压力的影响 系统和向酒精依赖的过渡。翻译依赖度量法也用于 将采用临床神经行为研究部分(具体目标3)。《野兽》 MODEL/生化测量核心将使中心研究人员丰富解释能力 通过对行为、神经化学、神经内分泌和 导致酒精依赖的药代动力学过程。

The Animal Models/Biochemical Measurement Core of The Scripps Research Institute Alcohol Research Center (TSRI-ARC) will provide a variety of behavioral and bioanalytical services to meet the specific needs of the Center at large. The first goal of the Core is to provide animals engaged in excessive drinking or have a history of excessive drinking using the intermittent access to ethanol drinking (IAE) model or chronic ethanol-induced dependence (CEID) model to TSRI-ARC and Center at Large investigators (Specific Aim 1). In addition, the Core will also supervise all changes in equipment and procedures and any refinement of the current animal models to ensure that standardized procedures are used across all laboratories. The second goal of the Core is to perform biochemical measurements, such as blood alcohol, cortisol/corticosterone, and ACTH levels and brain amino acid and endocannabinoid content, in support of all Center-related projects by establishing state-of-the-art techniques and an efficient, user-friendly website to schedule services, monitor progress, and receive data (Specific Aim 2). Finally, the last goal of the Core is to further characterize the animal models of excessive drinking to be utilized by the TSRI-ARC, including exploring the effect of excessive drinking on the transition to dependence and the effect of excessive drinking on the brain stress system by characterizing the consequence of a history of binge drinking (IAE model) on the brain stress system and the transition to alcohol dependence. Translational dependent measures also used in the Clinical Neurobehavioral Research Component will be employed (Specific Aim 3). The Animal Models/Biochemical Measurement Core will enable Center investigators to enrich the interpretational power of their experiments through investigations of behavioral, neurochemical, neuroendocrine, and pharmacokinetic processes contributing to alcohol dependence.