Animal Models Core

动物模型核心

• 批准号: 8401630
• 负责人: George F. Koob
• 金额: $ 27.93万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-10 至 2017-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8401630
• 关键词: Alcohol consumption; Alcohol dependence; Alcohols; Amino Acids; Animal Model; Animals; Behavioral; Biochemical; Blood; Brain; Cellular Neurobiology; Chronic; Clinical; Corticosterone; Corticotropin; Data; Dependence; Drug Kinetics; Endocannabinoids; Ensure; Equipment; Ethanol; Goals; Heavy Drinking; Hydrocortisone; Investigation; Laboratories; Measurement; Measures; Modeling; Monitor; Neurosecretory Systems; Procedures; Process; Recording of previous events; Research; Research Institute; Research Personnel; Schedule; Services; Stress; System; Techniques; alcohol effect; alcohol research; binge drinking; meetings; neurobehavioral; neurochemistry; research study; user-friendly; web site

The Animal Models/Biochemical Measurement Core of The Scripps Research Institute Alcohol Research Center (TSRI-ARC) will provide a variety of behavioral and bioanalytical services to meet the specific needs of the Center at large. The first goal of the Core is to provide animals engaged in excessive drinking or have a history of excessive drinking using the intermittent access to ethanol drinking (IAE) model or chronic ethanol-induced dependence (CEID) model to TSRI-ARC and Center at Large investigators (Specific Aim 1). In addition, the Core will also supervise all changes in equipment and procedures and any refinement of the current animal models to ensure that standardized procedures are used across all laboratories. The second goal of the Core is to perform biochemical measurements, such as blood alcohol, cortisol/corticosterone, and ACTH levels and brain amino acid and endocannabinoid content, in support of all Center-related projects by establishing state-of-the-art techniques and an efficient, user-friendly website to schedule services, monitor progress, and receive data (Specific Aim 2). Finally, the last goal of the Core is to further characterize the animal models of excessive drinking to be utilized by the TSRI-ARC, including exploring the effect of excessive drinking on the transition to dependence and the effect of excessive drinking on the brain stress system by characterizing the consequence of a history of binge drinking (IAE model) on the brain stress system and the transition to alcohol dependence. Translational dependent measures also used in the Clinical Neurobehavioral Research Component will be employed (Specific Aim 3). The Animal Models/Biochemical Measurement Core will enable Center investigators to enrich the interpretational power of their experiments through investigations of behavioral, neurochemical, neuroendocrine, and pharmacokinetic processes contributing to alcohol dependence.

斯克里普斯研究所酒精研究的动物模型/生化测量核心 中心（TSRI-ARC）将提供各种行为和生物分析服务，以满足特定需求 整个中心。核心的第一个目标是为过度饮酒或酗酒的动物提供服务 使用间歇性乙醇饮用（IAE）模型或慢性酒精饮用（IAE）模型的过度饮酒史 酒精诱导依赖（CEID）模型，TSRI-ARC和大型研究中心（具体目标1）。 此外，核心还将监督设备和程序的所有更改以及 目前的动物模型，以确保在所有实验室使用标准化程序。第二 Core的目标是进行生化测量，例如血液酒精、皮质醇/皮质酮，以及 ACTH水平和大脑氨基酸和内源性大麻素含量，以支持所有与中心有关的项目， 建立最先进的技术和一个高效、方便用户的网站， 进度，并接收数据（具体目标2）。最后，核心的最后一个目标是进一步描述 TSRI-ARC使用的过度饮酒动物模型，包括探索 过度饮酒对依赖性的转化以及过度饮酒对脑应激的影响 系统通过描述酗酒史（IAE模型）对大脑应激的影响 系统和过渡到酒精依赖。翻译相关的措施也用于 将采用临床神经行为研究组件（具体目标3）。动物 模型/生化测量核心将使中心研究人员能够丰富解释能力 他们的实验通过调查行为，神经化学，神经内分泌， 药代动力学过程有助于酒精依赖。