Mouse Genetics Core Facility

小鼠遗传学核心设施

• 批准号: 8666023
• 负责人: Brian G Petrich
• 金额: $ 18.04万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8666023
• 关键词: A Mouse; Adhesives; Alleles; Animal Model; Biological Models; Biological Testing; Blood Platelets; Blood Vessels; Breeding; Cell Adhesion; Cell Culture Techniques; Cells; Clone Cells; Cloning; Complex; Core Facility; DNA; Development; Embryo; Embryology; Endothelial Cells; Extravasation; Fibroblasts; Gene Targeting; Gene Transfer; Gene Transfer Techniques; Genetic; Genomic DNA; Genotype; Injection of therapeutic agent; Leukocytes; Libraries; Maintenance; Microinjections; Modification; Mouse, Founder, Transgenic; Mus; Preparation; Process; Production; Protein Overexpression; Proteins; Reagent; Recombinants; Research Personnel; Resources; Serum; Services; Signal Transduction; Smooth Muscle Myocytes; Somatic Cell; Techniques; Thrombosis; Training; Transfection; Transgenic Animals; Transgenic Mice; Transgenic Organisms; Vascular Diseases; blastocyst; design; embryonic stem cell; fetal; homologous recombination; implantation; implementation research; mouse model; offspring; protein function; recombinase; screening; vector

Genetically-modified mice provide a tractable approach to test the biological significance of protein function in complex processes such as thrombosis and leukocyte extravasation. Core A will serve as a mouse genetics core facility to provide advice, training, and services to project investigators in the design and implementation of mouse gene targeting and transgenesis projects including ES cell culture, transfection and screening for homologous recombination, blastocyst injection, and characterization of germline transmitted alleles in founder lines. In addition, Core A will provide project investigators with access to a library of mice maintained by the Core including Cre expressing lines for conditional deletion or overexpression of proteins in vascular cells (platelets, endothelial cells, smooth muscle cells, several types of leukocytes).

转基因小鼠提供了一种易于处理的方法来测试蛋白质功能在复杂过程中的生物学意义，如血栓形成和白细胞外渗。核心A将作为小鼠遗传学核心设施，为项目研究人员提供设计和实施小鼠基因靶向和转基因项目的建议，培训和服务，包括ES细胞培养，同源重组的转染和筛选，囊胚注射，以及创始人品系中生殖系传播等位基因的表征。此外，Core A将为项目研究者提供访问Core维护的小鼠库的权限，包括Cre表达系，用于血管细胞（血小板、内皮细胞、平滑肌细胞、几种类型的白细胞）中蛋白质的条件性缺失或过表达。