权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mouse Genetics Core Facility

小鼠遗传学核心设施

基本信息

批准号：
7995815
负责人：
Brian G Petrich
金额：
$ 19.1万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-08-15 至 2015-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7995815
关键词：
A Mouse Adhesives Alleles Animal Model Biological Models Biological Testing Blood Platelets Blood Vessels Breeding Cell Culture Techniques Cells Clone Cells Cloning Complex Core Facility DNA Development Doctor of Philosophy Embryo Embryology Endothelial Cells Extravasation Fibroblasts Gene Targeting Gene Transfer Gene Transfer Techniques Genetic Genomics Genotype Injection of therapeutic agent Instruction Leukocytes Libraries Maintenance Microinjections Modification Mouse, Founder, Transgenic Mus Names Preparation Process Production Protein Overexpression Proteins Reagent Recombinants Research Personnel Resources Screening procedure Serum Services Signal Transduction Smooth Muscle Myocytes Somatic Cell Techniques Thrombosis Training Transfection Transgenic Animals Transgenic Mice Transgenic Organisms Universities blastocyst design embryonic stem cell fetal homologous recombination implantation implementation research mouse model offspring professor protein function recombinase vector

项目摘要

PROJECT SUMMARY (See instructions): Genetically-modified mice provide a tractable approach to test the biological significance of protein function in complex processes such as thrombosis and leukocyte extravasation. Core A will serve as a mouse genetics core facility to provide advice, training, and services to project investigators in the design and implementation of mouse gene targeting and transgenesis projects including ES cell culture, transfection and screening for homologous recombination, blastocyst injection, and characterization of germline transmitted alleles in founder lines. In addition. Core A will provide project investigators with access to a library of mice maintained by the Core including Cre expressing lines for conditional deletion or overexpression of proteins in vascular cells (platelets, endothelial cells, smooth muscle cells, several types of leukocytes).

项目总结（见说明）：转基因小鼠提供了一种易于处理的方法来测试蛋白质功能的生物学意义在血栓形成和白细胞外渗等复杂过程中。核心A将充当鼠标遗传学核心设施，为项目研究人员提供设计和实施小鼠基因打靶和转基因项目，包括ES细胞培养、转染和同源重组的筛选、胚泡注射和生殖系传播的表征在创始人线的等位基因。另外。核心A将为项目研究人员提供访问小鼠库的权限由包括Cre表达系的核心维持，用于蛋白质的条件性缺失或过表达血管细胞（血小板、内皮细胞、平滑肌细胞、几种类型的白细胞）。