Mouse Genetics Core Facility

项目总结（见说明）： 转基因小鼠提供了一种易于处理的方法来测试蛋白质功能的生物学意义 在复杂的过程中，如血栓形成和白细胞外渗。核心A将充当鼠标 遗传学核心设施，为项目研究人员提供设计和 实施小鼠基因打靶和转基因项目，包括ES细胞培养、转染和 同源重组的筛选、胚泡注射和生殖系传播的表征 在创始人线的等位基因。另外。核心A将为项目研究人员提供访问小鼠库的权限 由包括Cre表达系的核心维持，用于蛋白质的条件性缺失或过表达 血管细胞（血小板、内皮细胞、平滑肌细胞、几种类型的白细胞）。

PROJECT SUMMARY (See instructions): Genetically-modified mice provide a tractable approach to test the biological significance of protein function in complex processes such as thrombosis and leukocyte extravasation. Core A will serve as a mouse genetics core facility to provide advice, training, and services to project investigators in the design and implementation of mouse gene targeting and transgenesis projects including ES cell culture, transfection and screening for homologous recombination, blastocyst injection, and characterization of germline transmitted alleles in founder lines. In addition. Core A will provide project investigators with access to a library of mice maintained by the Core including Cre expressing lines for conditional deletion or overexpression of proteins in vascular cells (platelets, endothelial cells, smooth muscle cells, several types of leukocytes).