Harvesting specific plant metabolites from hairy root cultures using magnetized n

描述：植物产生多种有价值的生物活性代谢物，但在野生植物中通常浓度较低。这使得这些化合物的分离和纯化变得复杂和昂贵。申请公司Naprogenix Inc.已经开发出一种技术，可以在突变植物细胞培养中增加特定生物活性代谢物的产量，这有望减轻这一问题。然而，这种方法或任何其他使用转基因植物细胞的方法的价值都受到为了提取所需产品而浪费破坏植物细胞的必要性的限制。 如果能够从连续培养的细胞中获得产品，这个过程将更加高效。纳米颗粒通过内吞作用被细胞摄取，然后被胞外排出。它们也可以被改造成吸附特定的化学物质。这表明，特殊设计(功能化和磁化)的纳米颗粒可以用来重复地从活的植物细胞培养物中“获取”特定的代谢物。该公司的主要项目之一是设计在黄花毛状根培养中产生新的黄酮类化合物。申请人已经证明，功能化的二氧化硅纳米颗粒吸附类似的类黄酮类化合物，并将它们从这些植物细胞培养物中提取和移除，而不会影响培养物的生存能力。现在的目标是设计优化功能化的磁化二氧化硅纳米颗粒(由肯塔基大学的合作者)，从这些毛状根中提取特定的类黄酮类化合物。 第一阶段的具体目的是证明：(A)暴露在这些纳米颗粒中，将黄酮类化合物从毛状根的细胞内转移到细胞外介质中(B)这些细胞外的磁化纳米颗粒可以在磁场中浓缩(C)这些纳米颗粒通过植物细胞后，可以从这些纳米颗粒中洗脱和收集大量的黄酮类化合物。如果成功，那么在第二阶段，申请者将设计和测试纳米颗粒，用于从优化的植物细胞培养中获取其他生物活性代谢物。这些药物将包括非常高价值的化疗药物，如紫杉类和长春花碱。如果成功，这种方法将对利用植物细胞培养生产和分离高价值天然产品产生重大影响。纳米粒子和应用于植物细胞的收获技术是可申请专利的，对申请公司和大学具有重大的商业意义。

DESCRIPTION: Plants produce a wide variety of valuable bioactive metabolites, but these are commonly present in low concentrations in the wild-type plant. This makes the separation and purification of these compounds complicated and expensive. The applicant company, Naprogenix Inc, has developed a technology for increasing the yields of specific bioactive metabolites in mutant plant cell cultures, which promises to lessen this problem. However, the value of this, or any other approach using genetically-modified plant cells, is limited by the necessity of the wasteful destruction of the plant cells in order to extract the required products. The process would be more efficient if products could be harvested from cells in continuous culture. Nanoparticles are taken up by cells by endocytosis, and subsequently exocytosed. They can also be engineered to adsorb specific chemicals. This suggests that specifically-engineered (functionalized and magnetized) nanoparticles could be used to repeatedly "harvest" specific metabolites from living plant cell cultures. One of the company's major projects is designed to generate novel flavonoids in goldenrod hairy root cultures. The applicants have shown that functionalized silica nanoparticles adsorb similar flavonoids and that they are taken up and removed from these plant cell cultures without compromising the viability of the cultures. The objective is now to engineer optimally-functionalized magnetized silica nanoparticles (by collaborators at the University of Kentucky) to harvest specific flavonoids from these hairy roots. The specific aims of phase I are to show that (a) exposure to these nanoparticles translocate flavonoids from the intracellular compartment of hairy roots to the extracellular medium (b) these extracellular magnetized nanoparticles can be concentrated in a magnetic field (c) significant amounts of flavonoids can be eluted and collected from these nanoparticles following their passage through plant cells. If this is successful, then, in phase II, the applicants will design ad test nanoparticles for harvesting other bioactive metabolites from optimized plant cell cultures. These will include very high value chemotherapeutic agents, such as the taxoids and vinca alkaloids. If successful this approach should make a major impact on the use of plant cell cultures for the production and isolation of high value natural products. The nanoparticles, and the harvesting technologies as applied to plant cells, are patentable, and have major commercial implications for the applicant company and the University.