Characterization of amino acid transport- and signaling in the mosquito fat body

描述（由申请人提供）：世界上许多国家迫切需要具有新作用模式的新杀虫剂来改善公共卫生。该项目解决了几个重要的问题，营养运输和信号通路的脂肪体的黄热病蚊子，埃及伊蚊。我们将描述两种必需的蚊子阳离子氨基酸转运蛋白的作用模式，并测试和改进有前途的化合物来抑制阳离子氨基酸转运。我们将补充这些方法与硅蚊子脂肪体基因知识网络的发展，旨在确定阳离子氨基酸转运蛋白下游的氨基酸信号转导和转运级联的基因。拟议的工作将通过对蚊子中氨基酸转运和信号传导的知识的重大收获而证明对人类健康具有重要意义。氨基酸转运抑制剂有可能成为新一代的公共卫生杀虫剂。此外，这项研究将扩大我们的知识，阳离子氨基酸转运蛋白在昆虫的功能机制，了解人类和其他模式系统中的必需氨基酸转运蛋白的影响。

DESCRIPTION (provided by applicant): New insecticides with novel modes of action are urgently needed to improve public health in many countries around the world. This project addresses several important questions regarding nutrient transport and signaling pathways in the fat body of the yellow fever mosquito, Aedes aegypti. We will characterize the mode of action of two essential mosquito cationic amino acid transporters and test and improve promising compounds to inhibit cationic amino acid transport. We will complement these approaches with the development of an in silico mosquito fat body gene knowledge network designed to identify genes in the amino acid signaling and transport cascades downstream of cationic amino acid transporters. The proposed work will prove significant for human health through the significant gain of knowledge on amino acid transport and signaling in mosquitoes. Amino acid transport inhibitors have the potential to give rise to a new generation of public health insecticides. In addition, this study will expand our knowledge of the functional mechanism of cationic amino acid transporter in insects with implications for understanding essential amino acid transporters in humans and other model systems.