Characterization of amino acid transport- and signaling in the mosquito fat body

描述(由申请人提供)：世界上许多国家迫切需要具有新作用模式的新杀虫剂来改善公共卫生。该项目解决了有关黄热病蚊子埃及伊蚊脂肪体内营养物质运输和信号通路的几个重要问题。我们将表征两种重要的蚊子阳离子氨基酸转运蛋白的作用方式，并测试和改进有希望的化合物来抑制阳离子氨基酸转运蛋白。我们将通过开发电子蚊子脂肪体内基因知识网络来补充这些方法，该网络旨在识别氨基酸信号中的基因，并在阳离子氨基酸转运体下游运输级联。这项拟议的工作将通过在蚊子体内获得氨基酸运输和信号转导方面的重大知识，证明对人类健康具有重要意义。氨基酸转运抑制剂有可能催生新一代公共卫生杀虫剂。此外，这项研究将扩大我们对阳离子氨基酸转运蛋白在昆虫中的作用机制的认识，对于理解人类和其他模型系统中必需的氨基酸转运蛋白具有重要意义。

DESCRIPTION (provided by applicant): New insecticides with novel modes of action are urgently needed to improve public health in many countries around the world. This project addresses several important questions regarding nutrient transport and signaling pathways in the fat body of the yellow fever mosquito, Aedes aegypti. We will characterize the mode of action of two essential mosquito cationic amino acid transporters and test and improve promising compounds to inhibit cationic amino acid transport. We will complement these approaches with the development of an in silico mosquito fat body gene knowledge network designed to identify genes in the amino acid signaling and transport cascades downstream of cationic amino acid transporters. The proposed work will prove significant for human health through the significant gain of knowledge on amino acid transport and signaling in mosquitoes. Amino acid transport inhibitors have the potential to give rise to a new generation of public health insecticides. In addition, this study will expand our knowledge of the functional mechanism of cationic amino acid transporter in insects with implications for understanding essential amino acid transporters in humans and other model systems.