OMX Blaze Structured-Illumination Microscope for a Core Facility

适用于核心设施的 OMX Blaze 结构照明显微镜

• 批准号: 8640326
• 负责人: SCOT CHARLES KUO
• 金额: $ 60万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-16 至 2016-02-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640326
• 关键词: Address; Cell Nucleus; Cell physiology; Cells; Color; Confocal Microscopy; Core Facility; Data; Distant; Equipment; Fluorescence; Funding; Image; Imagery; Label; Lateral; Life; Lighting; Microscope; Organelles; Performance; Proteins; Resolution; Specificity; Speed; Structure; System; Technology; Testing; Tissues; United States National Institutes of Health; Vendor; instrument; macromolecular assembly

DESCRIPTION (provided by applicant): As part of an established core facility, we request an OMX Blaze structured-illumination microscope (SIM) from Applied Precision. Key cellular processes occur in structures and assemblies smaller than resolvable by confocal microscopy, but still requiring the ease and specificity of fluorescence labeling. Addressing multiple experimental systems, our super-resolution needs have five constraints. (1) The volumes we must explore are often deep within cells and tissues, significantly distant from the coverslip. (2) All studies require axial super-resolution, as well as lateral super-resolution. (3) The relevant structures, spanning large protein assemblies to organelles to the nucleus, are typically macromolecular assemblies 100 nm or larger. (4) The assemblies often interact with many other molecules, requiring a robust palette of colors for simultaneous visualization. (5) For some live-cell dynamics, speed of imaging is important. After extensive testing of multiple technologies, only SIM is appropriate for our needs. Furthermore, we have preliminary SIM data for all Users, thoroughly exploring each vendor's implementation of SIM. Compared to existing equipment anywhere at Johns Hopkins, the proposed instrument will provide an order-of-magnitude superior performance that will enable key NIH-funded projects.

描述（由申请人提供）：作为一个既定的核心设施的一部分，我们要求从应用精密OMX火焰结构照明显微镜（SIM）。关键的细胞过程发生在结构和组件小于共聚焦显微镜可分辨，但仍然需要荧光标记的方便性和特异性。针对多个实验系统，我们的超分辨率需求有五个限制。(1)我们必须探索的体积通常位于细胞和组织的深处，距离盖玻片很远。（二） 所有研究都需要轴向超分辨率以及横向超分辨率。(3)相关的结构，跨越大型蛋白质组件到细胞器到细胞核，通常是100 nm或更大的大分子组件。(4)这些组件通常与许多其他分子相互作用，需要一个强大的调色板来同时可视化。(5)对于某些活细胞动力学，成像速度很重要。经过对多种技术的广泛测试，只有SIM卡适合我们的需求。此外，我们还拥有所有用户的SIM卡初步数据，全面了解每个供应商的SIM卡实施情况。与约翰霍普金斯任何地方的现有设备相比，拟议的仪器将提供数量级的上级性能，这将使NIH资助的关键项目。