Request for Zeiss LSM 510 MP Multiphoton Imaging System

描述（由申请人提供）：我们需要一台蔡司LSM 510MP显微镜，用于多种细胞，组织和生物体的多光子激发荧光成像。蔡司510MP是一个专用的多光子激发系统，因此补充了四个共聚焦显微镜，包括两个蔡司510-Meta共聚焦显微镜，已经在医学院显微镜设施。作为校内最大的核心显微镜设施，我们训练有素的员工和成熟的资源每年已经为250多名用户提供服务。然而，目前还没有多光子显微镜可以被美国国立卫生研究院资助的研究人员经常使用。由于若干原因，无法使用现有的文书。此外，激光技术的最新进展（光谱物理MaiTai DeepSee）极大地促进了多用户核心设施中最先进的多光子荧光显微镜。最初，该系统将为约翰霍普金斯大学多个部门的5个主要用户和潜在的60个次要用户提供服务。由于多光子荧光成像在活体样品中提供了前所未有的成像深度和灵敏度，我们预计小用户将慢慢成为大用户。得到广泛的兴趣、需求和大学的财政支持，并将服务于美国国立卫生研究院资助的大型科学界。

DESCRIPTION (provided by applicant): We request a Zeiss LSM 510MP microscope for the multiphoton-excited fluorescence imaging of a variety of cells, tissues and organisms. The Zeiss 510MP is a dedicated multiphoton-excitation system, thus complementing four confocal microscopes, including two Zeiss 510-Meta confocals, already in the School of Medicine Microscope Facility. As the largest core microscope facility on campus, our well-trained staff and established resources already serves over 250 users annually. However, there is no multiphoton microscope available for regular, general use by NIH-funded investigators. Existing instruments are inaccessible for a number of reasons. Furthermore, recent advances in laser technologies (Spectra-Physics MaiTai DeepSee) greatly facilitate state-of-the-art multiphoton fluorescence microscopy in a multi-user core facility. Initially, the system will serve 5 major users and potentially 60 minor users from multiple departments throughout Johns Hopkins. Because multiphoton fluorescent imaging provides unprecedented depth and sensitivity of imaging in living samples, we expect that minor users will slowly become major users. Supported by a broad base of interest, need, and financial support by the University, and will serve a large NIH-funded scientific community.