Request for Zeiss LSM 510 MP Multiphoton Imaging System

描述（由申请人提供）：我们要求Zeiss LSM 510 MP显微镜用于多种细胞、组织和生物体的多光子激发荧光成像。蔡司510 MP是一个专用的多光子激发系统，从而补充了四个共焦显微镜，包括两个蔡司510-Meta共焦，已经在医学院显微镜设施。作为校园内最大的核心显微镜设施，我们训练有素的员工和既定的资源每年已经为250多名用户提供服务。然而，没有多光子显微镜可供NIH资助的研究人员定期、一般使用。现有的文书由于若干原因而无法使用。此外，激光技术（Spectra-Physics MaiTai DeepSee）的最新进展极大地促进了多用户核心设施中最先进的多光子荧光显微镜。最初，该系统将为来自约翰霍普金斯多个部门的5个主要用户和潜在的60个次要用户提供服务。由于多光子荧光成像在活体样本中提供了前所未有的成像深度和灵敏度，我们预计小用户将慢慢成为主要用户。由大学的兴趣，需求和财政支持的广泛基础的支持，并将服务于一个大型NIH资助的科学界。

DESCRIPTION (provided by applicant): We request a Zeiss LSM 510MP microscope for the multiphoton-excited fluorescence imaging of a variety of cells, tissues and organisms. The Zeiss 510MP is a dedicated multiphoton-excitation system, thus complementing four confocal microscopes, including two Zeiss 510-Meta confocals, already in the School of Medicine Microscope Facility. As the largest core microscope facility on campus, our well-trained staff and established resources already serves over 250 users annually. However, there is no multiphoton microscope available for regular, general use by NIH-funded investigators. Existing instruments are inaccessible for a number of reasons. Furthermore, recent advances in laser technologies (Spectra-Physics MaiTai DeepSee) greatly facilitate state-of-the-art multiphoton fluorescence microscopy in a multi-user core facility. Initially, the system will serve 5 major users and potentially 60 minor users from multiple departments throughout Johns Hopkins. Because multiphoton fluorescent imaging provides unprecedented depth and sensitivity of imaging in living samples, we expect that minor users will slowly become major users. Supported by a broad base of interest, need, and financial support by the University, and will serve a large NIH-funded scientific community.