Request for Zeiss LSM 510 MP Multiphoton Imaging System

索取 Zeiss LSM 510 MP 多光子成像系统

• 批准号: 7388421
• 负责人: SCOT CHARLES KUO
• 金额: $ 50万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7388421
• 关键词: Arts; Cells; Communities; Complement; Core Facility; Depth; Financial Support; Funding; Image; Lasers; Life; Microscope; Minor; Multiphoton Fluorescence Microscopy; Numbers; Organism; Physics; Research Personnel; Resources; Sampling; System; Technology; Tissues; Training; Universities; base; fluorescence imaging; instrument; interest; medical schools

DESCRIPTION (provided by applicant): We request a Zeiss LSM 510MP microscope for the multiphoton-excited fluorescence imaging of a variety of cells, tissues and organisms. The Zeiss 510MP is a dedicated multiphoton-excitation system, thus complementing four confocal microscopes, including two Zeiss 510-Meta confocals, already in the School of Medicine Microscope Facility. As the largest core microscope facility on campus, our well-trained staff and established resources already serves over 250 users annually. However, there is no multiphoton microscope available for regular, general use by NIH-funded investigators. Existing instruments are inaccessible for a number of reasons. Furthermore, recent advances in laser technologies (Spectra-Physics MaiTai DeepSee) greatly facilitate state-of-the-art multiphoton fluorescence microscopy in a multi-user core facility. Initially, the system will serve 5 major users and potentially 60 minor users from multiple departments throughout Johns Hopkins. Because multiphoton fluorescent imaging provides unprecedented depth and sensitivity of imaging in living samples, we expect that minor users will slowly become major users. Supported by a broad base of interest, need, and financial support by the University, and will serve a large NIH-funded scientific community.

描述（由申请人提供）：我们要求Zeiss LSM 510 MP显微镜用于多种细胞、组织和生物体的多光子激发荧光成像。蔡司510 MP是一个专用的多光子激发系统，从而补充了四个共焦显微镜，包括两个蔡司510-Meta共焦，已经在医学院显微镜设施。作为校园内最大的核心显微镜设施，我们训练有素的员工和既定的资源每年已经为250多名用户提供服务。然而，没有多光子显微镜可供NIH资助的研究人员定期、一般使用。现有的文书由于若干原因而无法使用。此外，激光技术（Spectra-Physics MaiTai DeepSee）的最新进展极大地促进了多用户核心设施中最先进的多光子荧光显微镜。最初，该系统将为来自约翰霍普金斯多个部门的5个主要用户和潜在的60个次要用户提供服务。由于多光子荧光成像在活体样本中提供了前所未有的成像深度和灵敏度，我们预计小用户将慢慢成为主要用户。由大学的兴趣，需求和财政支持的广泛基础的支持，并将服务于一个大型NIH资助的科学界。

项目成果

OxLDL triggers retrograde translocation of arginase2 in aortic endothelial cells via ROCK and mitochondrial processing peptidase.

OXLDL通过岩石和线粒体加工肽酶在主动脉内皮细胞中精氨酸酶2的逆转转移。

• DOI: 10.1161/circresaha.115.304262
• 发表时间: 2014-08-01
• 期刊: Circulation research
• 影响因子: 20.1
• 作者: Pandey D;Bhunia A;Oh YJ;Chang F;Bergman Y;Kim JH;Serbo J;Boronina TN;Cole RN;Van Eyk J;Remaley AT;Berkowitz DE;Romer LH
• 通讯作者: Romer LH

Engineered heart slices for electrophysiological and contractile studies.

• DOI: 10.1016/j.biomaterials.2015.03.026
• 发表时间: 2015-07
• 期刊: Biomaterials
• 影响因子: 14
• 作者: Blazeski A;Kostecki GM;Tung L
• 通讯作者: Tung L

Optogenetic current in myofibroblasts acutely alters electrophysiology and conduction of co-cultured cardiomyocytes.

• DOI: 10.1038/s41598-021-83398-4
• 发表时间: 2021-02-24
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Kostecki GM;Shi Y;Chen CS;Reich DH;Entcheva E;Tung L
• 通讯作者: Tung L

Asymmetric inheritance of mTORC1 kinase activity during division dictates CD8(+) T cell differentiation.

• DOI: 10.1038/ni.3438
• 发表时间: 2016-06
• 期刊: Nature immunology
• 影响因子: 30.5
• 作者: Pollizzi KN;Sun IH;Patel CH;Lo YC;Oh MH;Waickman AT;Tam AJ;Blosser RL;Wen J;Delgoffe GM;Powell JD
• 通讯作者: Powell JD

Characterization of the specificity of O-GlcNAc reactive antibodies under conditions of starvation and stress.

• DOI: 10.1016/j.ab.2014.04.008
• 发表时间: 2014-07-15
• 期刊: Analytical biochemistry
• 影响因子: 2.9
• 作者: Reeves RA;Lee A;Henry R;Zachara NE
• 通讯作者: Zachara NE