Detection of Salmonella in Mouse Feces and Raw Pet Food Using ABI 7500 FAST Real-

项目总结 小鼠粪便和宠物生食中沙门氏菌的检测 ABI 7500快速实时定量聚合酶链式反应系统 迫切需要新的和有效的快速分析污染物的方法 在宠物食品的生产和分销环境中。 本项目的目标是扩展目前检测沙门氏菌的方法。 为建立和验证人类食品中沙门氏菌的检测方法 生宠物食品和老鼠粪便。 我们的理由是，拥有新的快速反应方法来检测其中一种 国家最流行的细菌将加强Vet-LIRN的能力，以缓解未来 爆发，迅速处理危机，教育公众，遏制病毒的传播 污染。 我们的具体目标包括： 1.对生宠物食品中沙门氏菌的检测方法进行多实验室验证 和老鼠的粪便 (我们的合作实验室包括爱荷华州立大学的兽医诊断实验室， 德克萨斯农工大学兽医学院动物诊断实验室 新泽西农业学院和明尼苏达大学兽医诊断实验室) 2.对确认沙门氏菌分离株的方法进行多实验室验证 3.对验证结果进行全面分析 在这项工程完成后，我们将知道以下情况： ？检测方法的灵敏度、特异度和可靠性百分比 ？该方法检测不同水平沙门氏菌的能力 ？与传统方法相比，结果的速度 沙门氏菌在不同条件下在生宠物食品和老鼠粪便中的活性 该项目将扩大Vet-LIRN实验室的能力，以提供快速反应 沙门氏菌污染事件。所获得的数据将有助于制定有效的 有助于预防未来与宠物饲料相关的沙门氏菌病的教育材料。它 将在保护和促进公共卫生以及 加强国家宠物食品安全和食品防御体系建设。这项研究还将 提高对与啮齿动物和生宠物食品有关的脆弱性的认识。

PROJECT SUMMARY Detection of Salmonella in Mouse Feces and Raw Pet Food Using ABI 7500 FAST Real-Time PCR System A critical need exists for new and validated methods of rapidly analyzing contaminants within the pet food production and distribution environments. The objective of this project is to expand a current method of detecting Salmonella in human food in order to develop and validate a method for detecting Salmonella in raw pet food and mouse feces. Our rationale is that having new quick-response methods of detection for one of the nation's most prevalent bacterium will strengthen the Vet-LIRN's ability to mitigate future outbreaks, swiftly handle crises, educate the public and, contain the spread of contamination. Our specific aims include the following: 1. Conduct a multi-lab validation of methods for detecting Salmonella in raw pet food and mouse feces (Our collaborating labs include, the Vet Diagnostic Lab at Iowa State University, the College of Vet Medicine at Texas A & M University, Animal Diagnostic Lab at New Jersey Dept of Ag, and the Vet Diagnostic Lab at the University of Minnesota) 2. Conduct a multi-lab validation of methodologies for confirming Salmonella isolates 3. Provide a comprehensive analysis of the validation results Upon completion of this project, we will know the following: ¿ the sensitivity, specificity, and percentage reliability of the detection method ¿ the method's ability to detect various levels of Salmonella ¿ the speed of results as compared to conventional methods ¿ the viability of Salmonella under various conditions in raw pet food & mouse feces This project will broaden the capacity of the Vet-LIRN labs to provide fast response to Salmonella contamination events. The data obtained will aid in developing effective education materials that will help prevent future pet-feed-associated salmonellosis. It will make significant advances in protecting and promoting public health as well as strengthening the nation's pet food safety and food defense system. This study will also create greater awareness of vulnerabilities related to rodents and raw pet food.