Excellence in Research: 2D Heterostructure Materials Based CRISPR Sensors for Detection of Salmonella and its serotypes

卓越研究：基于 2D 异质结构材料的 CRISPR 传感器，用于检测沙门氏菌及其血清型

• 批准号: 2301461
• 负责人: Ali Taheri
• 金额: $ 58.83万
• 依托单位: Tennessee State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-15 至 2026-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2301461&HistoricalAwards=false
• 关键词: Excellence; Research; 2D; Heterostructure; Materials

Foodborne diseases are a major public health concern all over the world, and it is important to develop methods that can quickly and accurately detect these diseases. The use of CRISPR-based systems is becoming popular for detecting these diseases, but they still rely on the amplification of target DNA. To address this issue, a new sensing platform will be developed based on 2D MXene-based heterostructure materials, which have unique electrochemical properties. This new platform can potentially detect pathogens in less than 15 minutes with superior sensitivity and specificity over existing platforms. If successful, this CRISPR sensor could enable rapid detection of Salmonella and other pathogenic agents without the need for pre-amplification steps. The system can also be easily and rapidly expanded for the detection of other emerging pathogenic agents and can assist policymakers in planning preventive measures. This project also offers opportunities for workforce development, including mentoring graduate and undergraduate students, offering workshops on nanomanufacturing CRISPR-based biosensors, and recruiting a team of high school students for competition at the iGEM international competition.The proposed project aims to develop a new sensing platform for the rapid detection of Salmonella using CRISPR-based systems and 2D MXene-based heterostructure materials. The project has three specific aims: Aim 1: Design specific gRNAs for Salmonella and its serotypes and evaluate their specificity; Aim 2: Develop a functional group to attach to the dCas9 enzyme and bind it to the surface terminal groups of the MXene-graphene materials; Aim 3: Prepare two types of CRISPR-MXene-graphene sensors based on either dCas9 or Cas12a enzymes, validate the sensors, and investigate the effect of bandgap and capacitance tuning on the sensing mechanism for different types of MXenes. The project's intellectual merit lies in advancing understanding of molecular interactions, chemical bonding, and electrochemical response in 2D heterostructure materials with tunable bandgap. The experimental methods developed from the proposed research can also help explore similar scientific problems in a wider range of the community, including the development of versatile platforms for rapid detection of other pathogenic agents. If successful, the proposed CRISPR-based MXene-graphene system could enable rapid detection of Salmonella and its serotypes without the need for pre-amplification steps. The system can be easily expanded for the detection of other pathogenic agents and can be useful for point-of-care applications in remote clinical laboratories. The project also has broader impacts, including enabling foundational technologies, workforce development, and strengthening HBCU research infrastructure.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

食源性疾病是世界各地的主要公共卫生问题，重要的是开发可以快速，准确检测这些疾病的方法。基于CRISPR的系统的使用正在流行，可用于检测这些疾病，但它们仍然依赖于目标DNA的扩增。为了解决这个问题，将基于具有独特的电化学性能的2D基因异质结构材料开发一个新的传感平台。这个新平台可以在不到15分钟的时间内检测病原体，其灵敏度和特异性比现有平台。如果成功的话，该CRISPR传感器可以快速检测沙门氏菌和其他致病剂，而无需进行预扩增的步骤。该系统也可以轻松，快速扩展，以检测其他新兴的致病剂，并可以帮助决策者计划预防措施。该项目还提供了劳动力发展的机会，包括指导研究生和本科生，提供有关基于CRISPR的纳米制造的生物传感器的研讨会，并招募一支高中生在IGEM国际竞赛中进行竞争。拟议的项目旨在为使用基于CRISPR的Systems使用基于CRISPR的Systems Systems和2D Mxene基于2D MXENE MXENE材料和2D MXENE HELESSTRUCTION的SALMONELLA开发新的Sensing Sensing平台。该项目具有三个特定的目标：目标1：为沙门氏菌及其血清型设计特定的GRNA，并评估其特异性；目标2：开发一个官能团以附着于DCAS9酶，并将其与Mxene-raphene材料的表面末端组结合； AIM 3：根据DCAS9或CAS12A酶准备两种类型的CRISPR-MXENE-raphene传感器，验证传感器，并研究带隙和电容调谐对不同类型MXENES的传感机理的影响。该项目的智力优点在于，在2D异质结构材料中，具有可调带隙的分子相互作用，化学键合和电化学响应的理解。根据拟议的研究开发的实验方法还可以帮助探索更广泛的社区范围内的类似科学问题，包括开发多功能平台，以快速检测其他病原剂。如果成功的话，拟议的基于CRISPR的MXENE-GRAPHENE系统可以快速检测沙门氏菌及其血清型，而无需进行放大前步骤。该系统可以轻松扩展以检测其他致病剂，并且可用于远程临床实验室的护理点应用。该项目还具有更广泛的影响，包括启用基础技术，劳动力发展和加强HBCU研究基础设施。该奖项反映了NSF的法定任务，并认为值得通过基金会的智力优点和更广泛的影响来通过评估来获得支持。