In Vivo Two-Photon Imaging of Cortical Activity in Alcohol-Dependent Mice

酒精依赖小鼠皮质活动的体内双光子成像

基本信息

  • 批准号:
    8635067
  • 负责人:
  • 金额:
    $ 21.49万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2014
  • 资助国家:
    美国
  • 起止时间:
    2014-03-15 至 2016-02-29
  • 项目状态:
    已结题

项目摘要

The goal of this project is to use in vivo two-photon laser scanning microscopy (2PLSM) to examine changes in neuronal activity in ethanol-dependent mice. Chronic drinking that leads to dependence is associated with marked changes in brain function including impairments in cortical processing and cognition. These impairments likely underlie the transition to heavy drinking and elucidating the mechanisms that underlie these changes is critical towards developing treatments and interventions that restore control over behavior. A key limitation in the study of the mechanistic actions on alcohol on brain function is the reliance upon in vitro techniques to monitor neuronal function. These approaches, while extremely valuable, are hindered by the loss of relevant sensory input and disruption of normal circuitry during preparation of the in vitro tissue sample. While brain imaging approaches can avoid this complication, they are compromised by a lack of specificity with regard to spatial resolution and neuronal sub-type. In this application, we will use in vivo 2PLSM to image neuronal activity and neurovascular function in alcohol-dependent mice. In Aim 1, we will utilize a recently developed line of mice that, when crossed with a specific Cre-driver mouse line, expresses the calcium-sensor protein GCaMP3 in a defined sub-population of neurons. Neuronal calcium dynamics will be monitored in glutamatergic pyramidal neurons and fast-spiking interneurons by crossing the GCaMP3 mice with the Wfs1-Tg2-CreERT2 line and Pvalb-2A-Cre line; respectively. Activity will be monitored both during rest and during presentation of visual cues that reliably induce activity in visual cortex neurons. In Aim 2, neurovascular coupling (e.g., activity-dependent changes in local brain blood flow) will be monitored by the use of a dye recently shown by the Co-I to effectively signal changes in arteriolar blood flow. Together, results from these two complementary aims will establish the technique of in vivo 2PLSM in the study of alcohol action and will support future studies designed to analyze the effects of alcohol on cortical function.
本项目的目标是使用体内双光子激光扫描显微镜(2PLSM)

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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JOHN J. WOODWARD其他文献

JOHN J. WOODWARD的其他文献

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{{ truncateString('JOHN J. WOODWARD', 18)}}的其他基金

Role of GluN2A and MMPs in the CeA in Dependence-Induced Escalation of Etoh Drinking
CeA 中 GluN2A 和 MMP 在 Etoh 饮酒依赖性升级中的作用
  • 批准号:
    10525274
  • 财政年份:
    2022
  • 资助金额:
    $ 21.49万
  • 项目类别:
Role of GluN2A and MMPs in the CeA in Dependence-Induced Escalation of Etoh Drinking
CeA 中 GluN2A 和 MMP 在 Etoh 饮酒依赖性升级中的作用
  • 批准号:
    10675691
  • 财政年份:
    2022
  • 资助金额:
    $ 21.49万
  • 项目类别:
In Vivo Two-Photon Imaging of Cortical Activity in Alcohol-Dependent Mice
酒精依赖小鼠皮质活动的体内双光子成像
  • 批准号:
    8821559
  • 财政年份:
    2014
  • 资助金额:
    $ 21.49万
  • 项目类别:
Pilot Project
试点项目
  • 批准号:
    8128146
  • 财政年份:
    2011
  • 资助金额:
    $ 21.49万
  • 项目类别:
RC3 EFFECTS OF ACUTE AND CHRONIC ALCOHOL ON ORBITOFRONTAL CORTEX FUNCTION
RC3 急性和慢性酒精对眶额皮层功能的影响
  • 批准号:
    8128132
  • 财政年份:
    2011
  • 资助金额:
    $ 21.49万
  • 项目类别:
Ethanol Sensitivity of Native and Cloned NMDA Receptors
天然和克隆 NMDA 受体的乙醇敏感性
  • 批准号:
    7865896
  • 财政年份:
    2009
  • 资助金额:
    $ 21.49万
  • 项目类别:
Ethanol and Persistent Activity in Prefrontal Cortex
乙醇与前额皮质的持续活动
  • 批准号:
    7533007
  • 财政年份:
    2007
  • 资助金额:
    $ 21.49万
  • 项目类别:
Ethanol and Persistent Activity in Prefrontal Cortex
乙醇与前额皮质的持续活动
  • 批准号:
    7226878
  • 财政年份:
    2005
  • 资助金额:
    $ 21.49万
  • 项目类别:
Neural Actions of Toluene
甲苯的神经作用
  • 批准号:
    6625672
  • 财政年份:
    2002
  • 资助金额:
    $ 21.49万
  • 项目类别:
Neural Actions of Toluene
甲苯的神经作用
  • 批准号:
    7759514
  • 财政年份:
    2002
  • 资助金额:
    $ 21.49万
  • 项目类别:

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