Sensitive and Quantitative MS-bases Glycomic Mapping Platform

描述(申请人提供)：由于迫切需要全面了解糖基化在许多重要生物学功能中的生物学属性，如免疫反应、细胞发育、细胞分化/黏附和宿主-病原体相互作用，糖组学和糖蛋白组学仍然是高度活跃和有趣的研究领域。此外，几十年来，异常的糖基化被认为是许多哺乳动物疾病的属性，包括骨关节炎、囊性纤维化和癌症。多糖的不同生物学作用及其在疾病中的影响产生了对可靠的定量糖学方法的需求，从而允许对生物系统中的多糖进行灵敏的监测。这项建议的目的是建立一个糖链图谱平台，能够自动识别、注释和定量来自生物样品的多糖。该平台包括分析方法和生物信息学工具。分析方法以反相液相色谱和全甲基化多聚糖的质谱学为基础，而生物信息学工具促进了解释和定量的自动化。糖链图谱平台将被用来了解糖链在癌症和心血管疾病等疾病发展中的生物学属性。

DESCRIPTION (provided by applicant): Because of the pressing needs to comprehensively understand the biological attributes of glycosylation in many important biological functions such as the immune response, cell development, cellular differentiation/adhesion and host-pathogen interactions, glycomics and glycoproteomics continue to be highly dynamic and interesting research areas. Moreover, aberrant glycosylation for decades has been recognized as the attribute of many mammalian diseases, including osteoarthritis, cystic fibrosis and cancer. The diverse biological roles of glycans and their implications in diseases have created a demand for reliable quantitative glycomic approaches, permitting sensitive monitoring of glycans in biological systems. The aim of this proposal is the creation of glycomic mapping platform enabling the automated identification, annotation and quantitation of glycans derived from biological samples. The platform comprises of both analytical methods and bioinformatic tools. The analytical methods are based on reversed-phase liquid chromatography and mass spectrometry of permethylated glycans while the bioinformatic tools facilitate automation of interpretation and quantitation. The glycomic mapping platform will be employed to understand the biological attributes of glycan in disease development, such as cancer and cardiovascular diseases.