Sensitive and Quantitative MS-bases Glycomic Mapping Platform

描述（由申请人提供）：由于迫切需要全面了解糖基化在许多重要生物学功能中的生物学特性，如免疫反应、细胞发育、细胞分化/粘附和宿主-病原体相互作用，糖组学和糖蛋白组学一直是高度动态和有趣的研究领域。此外，几十年来，异常糖基化已被认为是许多哺乳动物疾病的特征，包括骨关节炎、囊性纤维化和癌症。聚糖的多种生物学作用及其在疾病中的意义产生了对可靠的定量糖偶联方法的需求，允许对生物系统中的聚糖进行敏感监测。本提案的目的是创建糖链图谱平台，实现生物样品中糖链的自动识别、注释和定量。该平台包括分析方法和生物信息学工具。分析方法基于过甲基化聚糖的反相液相色谱和质谱分析，而生物信息学工具促进了解释和定量的自动化。glycomic制图平台将用于了解聚糖在疾病发展中的生物学特性，如癌症和心血管疾病。

DESCRIPTION (provided by applicant): Because of the pressing needs to comprehensively understand the biological attributes of glycosylation in many important biological functions such as the immune response, cell development, cellular differentiation/adhesion and host-pathogen interactions, glycomics and glycoproteomics continue to be highly dynamic and interesting research areas. Moreover, aberrant glycosylation for decades has been recognized as the attribute of many mammalian diseases, including osteoarthritis, cystic fibrosis and cancer. The diverse biological roles of glycans and their implications in diseases have created a demand for reliable quantitative glycomic approaches, permitting sensitive monitoring of glycans in biological systems. The aim of this proposal is the creation of glycomic mapping platform enabling the automated identification, annotation and quantitation of glycans derived from biological samples. The platform comprises of both analytical methods and bioinformatic tools. The analytical methods are based on reversed-phase liquid chromatography and mass spectrometry of permethylated glycans while the bioinformatic tools facilitate automation of interpretation and quantitation. The glycomic mapping platform will be employed to understand the biological attributes of glycan in disease development, such as cancer and cardiovascular diseases.