Sensitive and Quantitative MS-bases Glycomic Mapping Platform

基于 MS 的灵敏定量糖组图谱平台

• 批准号: 8927045
• 负责人: Yehia Mechref
• 金额: $ 27.69万
• 依托单位: TEXAS TECH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-15 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8927045
• 关键词: Adhesions; Area; Automated Annotation; Automation; Bioinformatics; Biologic Characteristic; Biological; Biological Process; Cardiovascular Diseases; Cell Differentiation process; Cells; Chromatography; Cystic Fibrosis; Degenerative polyarthritis; Development; Disease; Disease Progression; Esophageal Diseases; Glioma; Glycoproteins; Goals; HIV; HIV Envelope Protein gp120; Health; High temperature of physical object; Hydrophobicity; Immune response; Isomerism; Label; Lipids; Liquid Chromatography; Liver diseases; Malignant Neoplasms; Maps; Mass Spectrum Analysis; Metastatic malignant neoplasm to brain; Methods; Molecular Weight; Monitor; Neuroblastoma; Phase; Polysaccharides; Protein Glycosylation; Proteins; Reagent; Research; Research Activity; Role; Sampling; Serum; Structure; Systems Biology; Techniques; Time; Validation; analytical method; base; biological systems; cellular development; comparative; glycosylation; interest; ionization; liquid chromatography mass spectrometry; malignant breast neoplasm; methyl iodide; pathogen; tool

DESCRIPTION (provided by applicant): Because of the pressing needs to comprehensively understand the biological attributes of glycosylation in many important biological functions such as the immune response, cell development, cellular differentiation/adhesion and host-pathogen interactions, glycomics and glycoproteomics continue to be highly dynamic and interesting research areas. Moreover, aberrant glycosylation for decades has been recognized as the attribute of many mammalian diseases, including osteoarthritis, cystic fibrosis and cancer. The diverse biological roles of glycans and their implications in diseases have created a demand for reliable quantitative glycomic approaches, permitting sensitive monitoring of glycans in biological systems. The aim of this proposal is the creation of glycomic mapping platform enabling the automated identification, annotation and quantitation of glycans derived from biological samples. The platform comprises of both analytical methods and bioinformatic tools. The analytical methods are based on reversed-phase liquid chromatography and mass spectrometry of permethylated glycans while the bioinformatic tools facilitate automation of interpretation and quantitation. The glycomic mapping platform will be employed to understand the biological attributes of glycan in disease development, such as cancer and cardiovascular diseases.

描述（由申请人提供）：由于迫切需要全面了解糖基化在许多重要生物学功能（如免疫应答、细胞发育、细胞分化/粘附和宿主-病原体相互作用）中的生物学属性，糖组学和糖蛋白组学仍然是高度动态和有趣的研究领域。此外，几十年来，异常糖基化已被认为是许多哺乳动物疾病的属性，包括骨关节炎、囊性纤维化和癌症。聚糖的不同生物学作用及其在疾病中的意义已经产生了对可靠的定量糖组学方法的需求，从而允许对生物系统中的聚糖进行灵敏的监测。该提案的目的是创建糖组学图谱平台，从而能够自动识别、注释和定量来自生物样品的聚糖。该平台包括分析方法和生物信息学工具。分析方法基于全甲基化聚糖的反相液相色谱法和质谱法，而生物信息学工具有助于自动化解释和定量。糖组学图谱平台将用于了解疾病发展中聚糖的生物学属性，如癌症和心血管疾病。