A Comparative Study of M. tuberculosis and M. bovis BCG of T cell responses

结核分枝杆菌和牛分枝杆菌卡介苗T细胞反应的比较研究

• 批准号: 8677401
• 负责人: Patricia Grace
• 金额: $ 4.27万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-03-01 至 2016-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8677401
• 关键词: Address; Aerosols; Animal Housing; Animals; Antigen Presentation; Antigen-Presenting Cells; Antigens; Award; Bacteria; Behavior; Biology; CD4 Positive T Lymphocytes; CD8B1 gene; Cataloging; Catalogs; Cell Communication; Cells; Characteristics; Clinical; Comparative Study; Cultured Cells; Data; Dendritic Cells; Development; Disease; Dry Ice; Elevator; Environmental air flow; Floods; Frequencies; Goals; HIV; Health; Histocompatibility Antigens Class II; House mice; Housing; Human; Hurricane; Image; Immune; Immune response; Immunity; Immunocompetent; Immunology; In Vitro; Infection; Inferior; Laboratories; Life; Location; Lung; Mechanics; Medical center; Methods; Mus; Mycobacterium tuberculosis; Pharmaceutical Preparations; Plumbing; Process; Public Health; Publications; Reagent; Recording of previous events; Reporting; Research; Running; Safety; Sampling; Site; Sterility; T cell response; T-Lymphocyte; Techniques; Testing; Time; Training; Tuberculosis; Tuberculosis Vaccines; Update; Virulent; Water; Work; adaptive immunity; base; biomedical scientist; biosafety level 3 facility; career; in vivo; indexing; innovation; macrophage; prevent; public health research; research study; residence; tissue culture; tuberculosis immunity

On October 29, 2012, the NYU Langone Medical Center was hit by the Superstorm Sandy, which caused significant damages to the facilities used to conduct my research. Hurricane Sandy caused storm surges that exceeded what our facilities could withstand, leading to significant flooding. The repercussions of this flooding led to mechanical, electrical, and plumbing damages in many of the research buildings at NYU. These damages led to immediate loss of experiments and reagents but have also long-term delays in the completion of this project. In the wake of Hurricane Sandy, access to all research buildings was limited due to health and safety issues. Smilow Research Building, our main laboratory location, had no power for a week post-Sandy, which meant that freezers and refrigerators containing reagents we use routinely for experimentation were lost. Full-power and unrestricted access to the Smilow Research Building was not restored until one month post-Sandy. After the re-opening of Smilow, an additional 2 months of our time was dedicated to cataloging research losses and purchasing new reagents for experimentation. The Berg Building, which houses our Biosafety Level 3 (ABSL3) facility, remains without power. This facility housed animals infected with M. tuberculosis (M.tb) and is where we processed all infected samples prior to the storm. All infectious samples from on-going experiments were stored in refrigerators and freezers within the ABSL3 facility. We were able to save bacterial strains stored within our -80C freezer by supplying it with dry ice, however many other samples from on-going experiments could not be saved. The loss of these experimental samples is equal to a loss of three 6-week experiments. Work with the infected animals of the ongoing experiments at that time was significantly delayed in the absence of a proper ABSL3 facility and prevented the analysis of samples at key time points during infection. The ABSL3 located in Berg remains without power, ventilation, running water, or elevator access; the reopening of this facility is projected to be in September of this year, which will make it nearly a year post-Sandy, that this facility has been shut down. In the meantime we have made use of a second BSL3 facility within the Smilow Research Building, which is now operational on a limited basis to resume M. tuberculosis experiments. This facility was suited for in vitro work, with one Tissue Culture (TC) hood available for TB work, which was a huge bottleneck for progress, since amongst the TB labs at NYU we went from 4 available TC hoods to 1 available hood for experimentation. With the exception of Aim 1 of this project, all other proposed experiments for this award require mouse infections with M. tuberculosis. In April 2013, 6 months post-Sandy, we were able to expand to 2 TC hoods (shared by 15 people), it was also at this time that we were able to relocate the aerosol unit from our ABSL3 in the Berg Building to this Smilow BSL3. A satellite mouse housing facility has been built to accommodate our need for infected animal housing post-Sandy. To-date, we are ~20% operational post-Sandy.

2012年10月29日，纽约大学朗格尼医疗中心受到超级风暴桑迪的袭击，造成 对用来进行我的研究的设施造成重大破坏。飓风桑迪引发的风暴潮 超出了我们的设施所能承受的范围，导致了严重的洪水。这场洪灾的影响 导致纽约大学许多研究大楼的机械、电气和管道损坏。这些 损坏立即导致实验和试剂的损失，但也造成了长期的延迟完成 这个项目的。 飓风桑迪过后，由于健康和安全问题，所有研究大楼的出入都受到限制。 我们的主要实验室斯米洛研究大楼在桑迪飓风过后的一周内停电，这意味着 装有我们经常用于实验的试剂的冰柜和冰箱都不见了。动力十足 直到桑迪飓风过后一个月，才恢复对斯米洛研究大楼的不受限制的访问。之后 Smilow的重新开放，我们另外两个月的时间专门用于编目研究损失和 购买用于实验的新试剂。 我们的生物安全3级(ABSL3)设施所在的伯格大楼仍然没有电力供应。这个设施 饲养感染结核分枝杆菌(M.tb)的动物，在此之前我们处理所有受感染的样本 暴风雨。所有来自正在进行的实验的感染样本都储存在冰箱和冰柜中 ABSL3设施。我们用干燥的液体保存了我们-80C冰柜里的细菌菌株。 冰，然而，来自正在进行的实验的许多其他样本无法保存。这些东西的损失 实验样本相当于三次为期6周的实验的损失。与受感染的动物一起工作 当时正在进行的实验在缺乏适当的ABSL3设施的情况下被显著推迟，并且 防止在感染期间的关键时间点对样本进行分析。位于伯格的ABSL3仍然存在 没有电力、通风、自来水或电梯通道；该设施的重新开放预计将在 今年9月，这将使桑迪事件发生近一年后，该设施已被关闭。 与此同时，我们利用了斯米洛研究大楼内的第二个BSL3设施，即 目前在有限的基础上运作，以恢复结核分枝杆菌的实验。该设备适用于体外培养 工作，一个组织培养(TC)罩可用于结核病工作，这是进展的一个巨大瓶颈， 因为在纽约大学的结核病实验室中，我们从4个可用的TC风帽到1个可用的风帽进行实验。 除本项目的目标1外，所有其他为该奖项提出的实验都需要鼠标 结核分枝杆菌感染。在2013年4月，也就是桑迪事件发生6个月后，我们能够扩展到2个TC引擎盖 (15人共享)，也是在这个时候，我们能够将气雾剂装置从我们的ABSL3搬迁到 从伯格大厦到这个斯米洛BSL3。已经建造了一个卫星老鼠收容所来容纳我们的 桑迪之后需要感染动物的住所。到目前为止，我们在桑迪事件后的运营比例约为20%。