Regulation of adult stem cell homeostatic response to inflammatory injury

成体干细胞稳态反应对炎症损伤的调节

• 批准号: 8610878
• 负责人: Xiaonan Han
• 金额: $ 19.24万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-04 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8610878
• 关键词: Adult; Cell Differentiation process; Cells; Chronic; Clinical Investigator; Data; Development; Diarrhea; Disease; Engraftment; Enterocytes; Environmental Risk Factor; Epithelial; Epithelial Cells; Epithelium; Exploratory/Developmental Grant; Functional disorder; Genes; Genetic; Genetic Engineering; Genetic Variation; Healed; Homeostasis; Human; Immune; Impairment; Implant; Infection; Inflammatory; Inflammatory Bowel Diseases; Inflammatory Response; Injury; Intercellular Junctions; Intestines; JAK2 gene; Link; Mediating; Medical; Mucositis; Mus; Natural regeneration; Organoids; Pathway interactions; Pluripotent Stem Cells; Production; Regulation; Reporting; Research; Role; STAT5A gene; Scientist; Signal Transduction; Staging; Stem cells; System; Testing; Therapeutic; Tight Junctions; Tissue Engineering; Tissue Therapy; Tissues; Transgenic Organisms; adult stem cell; cytokine; genome wide association study; healing; innovation; loss of function; monolayer; mouse model; notch protein; novel; prevent; public health relevance; response; stem cell biology; stem cell differentiation; stem cell division; stem cell therapy; tissue repair; tool; transcription factor

DESCRIPTION (provided by applicant): Environmental factors such as microbiota or infection trigger immune-mediated tissue injury, which leads to chronic mucosal diseases in the genetically susceptible subjects, such as Inflammatory Bowel Diseases (IBD). Stem cell therapy has recently achieved many successful cases in the personalized treatment of IBD, but intestinal epithelial stem cell (IESC) treatment is still at an early stage. JAK-STAT activation wa suggested to promote adult intestinal stem cell division and differentiation, respectively, and thereby could drive the renewal of intestinal epithelial cells (IEC). We recently reported that STAT5 signaling prevents intestinal barrier dysfunction and promotes mucosal healing in colitic mouse models. Furthermore, our preliminary data indicated that deletion of STAT5 decreased the expression of stem cell markers Lgr5, Ascl2, and Olfm4, and inhibited NOTCH activation and cellular junction maturation in adult IESC-derived organoid. We therefore hypothesize that STAT5 signaling controls adult IESC activity via regulation of a stem cell gene signature to promote intercellular junction formation; activation of STAT5 signaling enhances IEC organoid engraftment against infection-induced intestinal barrier dysfunction. We will test our hypotheses with the following two Aims. Our Aim 1 will determine the role of STAT5 signaling for adult IESC differentiation. The studies in this Aim will, first, inducibly delete IEC STAT5 or hyperactivate IEC STAT5 in mice to characterize IESC homeostasis, stem cell gene profiles, and Wnt/Notch signaling in adult mouse intestines. Second, using human pluripotent stem cells-derived intestinal organoids, we will genetically manipulate STAT5 expression to define STAT5's role in the regulation of Wnt/Notch signaling during IESC differentiation. Our Aim 2 will define the requirement of STAT5 signaling for differentiation-associated development of IEC monolayers. First, we will characterize tight junction development using both STAT5 deficient and STAT5 inducible IESC-derived organoids. Second, we will culture these mouse organoids to implant the recipient control mice to assess IESC integrity and tight junction formation. Overall, we will determine that epithelial STAT5 regulates a stem cell gene signature via interplay with NOTCH signaling to promote intercellular junction reformation for healing mucosal inflammation. Our studies will demonstrate an essential role for STAT5 in the regulation of adult stem cell homeostasis via a stem cell gene signature, and will explore a novel engineered tissue therapy that directly heals the epithelial barrier disruption during mucosal inflammation. We propose a paradigm shift for this research field from understanding STAT5 signaling as an essential regulator of stem cell biology toward developing the potential therapeutic tool. We believe this exploratory R21 mechanism will permit a collaborative team of basic scientists and clinical investigators to start a brand-new project, and explore a novel potential therapeutic avenue for restoring epithelial barrier dysfunction in mucosal inflammation.

描述（由申请人提供）：环境因素（如微生物群或感染）触发免疫介导的组织损伤，导致遗传易感受试者的慢性粘膜疾病，如炎症性肠病（IBD）。近年来，干细胞治疗在IBD的个性化治疗中取得了许多成功的案例，但肠上皮干细胞（IESC）治疗仍处于早期阶段。JAK-STAT的激活分别促进成体肠干细胞的分裂和分化，从而驱动肠上皮细胞的更新。我们最近报道了STAT 5信号传导在结肠炎小鼠模型中预防肠屏障功能障碍并促进粘膜愈合。此外，我们的初步数据表明，STAT 5的缺失降低了干细胞标志物Lgr 5，Ascl 2和Olfm 4的表达，并抑制了成体IESC衍生的类器官中的NOTCH激活和细胞连接成熟。因此，我们假设STAT 5信号通过调节干细胞基因特征以促进细胞间连接形成来控制成人IESC活性; STAT 5信号的激活增强IEC类器官移植对抗感染诱导的肠屏障功能障碍。我们将通过以下两个目标来检验我们的假设。我们的目标1将确定STAT 5信号传导在成人IESC分化中的作用。该目的中的研究将首先在小鼠中诱导缺失IEC STAT 5或过度激活IEC STAT 5，以表征成年小鼠肠道中的IESC稳态、干细胞基因谱和Wnt/Notch信号传导。其次，使用人多能干细胞衍生的肠类器官，我们将遗传操纵STAT 5表达，以确定STAT 5在IESC分化期间调节Wnt/Notch信号传导中的作用。我们的目标2将定义IEC单层分化相关发展的STAT 5信号传导的要求。首先，我们将使用STAT 5缺陷型和STAT 5诱导型IESC衍生的类器官来表征紧密连接的发育。其次，我们将培养这些小鼠类器官以植入受体对照小鼠，以评估IESC完整性和紧密连接形成。总的来说，我们将确定上皮细胞STAT 5通过与NOTCH信号传导相互作用来调节干细胞基因签名，以促进细胞间连接重建，从而愈合粘膜炎症。我们的研究将证明STAT 5在通过干细胞基因签名调节成体干细胞稳态中的重要作用，并将探索一种新的工程组织疗法，直接治愈粘膜炎症期间的上皮屏障破坏。我们提出了该研究领域的范式转变，从理解STAT 5信号传导作为干细胞生物学的重要调节因子转向开发潜在的治疗工具。我们相信，这种探索性的R21机制将允许基础科学家和临床研究人员的合作团队开始一个全新的项目，并探索一种新的潜在治疗途径，用于恢复粘膜炎症中的上皮屏障功能障碍。