NHGRI/DIR Flow Cytometry Core

NHGRI/DIR 流式细胞仪核心

基本信息

项目摘要

The Flow Cytometry Core Facility of the National Human Genome Research Institute has the goal of providing all NHGRI investigators with access to high quality flow cytometry services. The Core serves to enhance the scope and quality of scientific research performed by the Institute. For FY2014 the Flow Cytometry Core was staffed by Stacie Anderson (Core Director, 100% effort, GMBB), and Martha Kirby (100% effort, GMBB). The Flow Cytometry Core is staffed Monday through Friday. Access to the Flow Core after regular working hours and on weekends/holidays is possible for authorized investigators through Accutech card readers. The NHGRI Flow Cytometry Core currently maintains two BD FACSArias that are primarily used for sorting cells. Basically the Arias and the LSRII have the same laser and filter configurations. However one Aria has an additional 561nm laser, which addresses the need to discriminate fluorochromes, such as PE and fluorescent proteins, such as DsRed and mCherry. Each FACSAria and LSRII is configured with three lasers/four and can measure up to nine/thirteen fluorescent parameters as well as physical parameters (size and granularity). Ideally, the LSRII should have a 561nm laser so that initial analysis experiments could be performed on the LSRII rather than on the Aria, which is typically dedicated to the isolation of cells. Among the various applications over the past year, these sorting capabilities have been used for isolation of tissue cell populations for animal transplantation experiments; isolation of blood cell sub-populations for analysis of functional cellular properties and gene transcription profiles, as well as high throughput screening of enhancer regions. The FACSArias typically have a two to three week sign up waiting time and are currently used at capacity. For direct flow cytometry analysis applications not involving cell sorting, the Flow Core offers one BD FACSCalibur and one BD LSRII. The FACSCalibur can measure up to four fluorescent parameters, and are routinely used for data acquisition and pre-sort analysis. Typical uses of the FACSCalibur include analysis of GFP expression and cell cycle, as well as mutagenesis screening. The BD LSRII uses digital electronics and Diva software similar to the Aria and is used to perform 9-color analyses. This allows investigators to characterize cells in more detail before sorting on the Aria. The instrument is equipped with a High Throughput Sample (HTS) device that gives the investigator the ability to analyze many samples in 96 well plate format without the need of sitting at the instrument during the acquisition procedure. The BD LSRII is available during regular Core hours as well as after hours and on weekends. The Flow Core maintains a laser scanning cytometer (LCS). The LSC uses laser-based opto-electronics and automated analysis capabilities to simultaneously and rapidly measure biochemical constituents and evaluate cell morphologies. Current applications (100%) are focused on zebra fish projects including drug screens and blood development. The Flow Core also maintains a Miltenyi Auto MACS that is used for magnetic cell separations. The AutoMACS is often used as a pre-enrichment step prior to sorting on a flow cytometer. In September 2014 the autoMACS will be replaced with a new autoMACS PRO Separator that replaces the original model. The autoMACS Pro Separator is a bench top instrument for high-speed magnetic cell sorting of multiple samples. Employing MACS Technology, the autoMACS Pro Separator is designed for cell isolation in a fully automated, walk-away fashion. Over the past year, the services and capabilities of the NHGRI Flow Core have been taken advantage of by over 60 trainees from 5 Branches/14 Sections in the Institute (Table 1). July 2014 LSRII 234/268 scheduled appointments Calibur 638/406 scheduled appointments Arias 339/362 scheduled appointments icys 12/8 scheduled appointments TOTAL scheduled appointments for FY2014 through July, 2014 1223/1044* scheduled appointments *(Includes a one month government shutdown) In addition, the Flow Core continued to maintain CLIA accreditation in support of immunophenotyping and protein expression studies used in NHGRI clinical research protocols. (Current accreditation March 2014 through March 2016) The BD LSRII uses digital electronics and Diva software similar to the Aria and is used to perform 9-color analyses. This allows investigators to characterize cells in more detail before sorting on the Aria. The instrument is equipped with a High Throughput Sample (HTS) device that gives the investigator the ability to analyze many samples in 96 well plate format without the need of sitting at the instrument during the acquisition procedure. The BD LSRII is available during regular Core hours as well as after hours and on weekends. The Flow Core maintains a laser scanning cytometer (LCS). The LSC uses laser-based opto-electronics and automated analysis capabilities to simultaneously and rapidly measure biochemical constituents and evaluate cell morphologies. Current applications (100%) are focused on zebra fish projects including drug screens and blood development. The Flow Core also maintains a Miltenyi Auto MACS that is used for magnetic cell separations. The AutoMACS is often used as a pre-enrichment step prior to sorting on a flow cytometer. Over the past year, the services and capabilities of the NHGRI Flow Core have been taken advantage of by over 60 trainees from 5 Branches/14 Sections in the Institute LSRII 265 scheduled appointments Calibur 551 scheduled appointments Arias 327 scheduled appointments icys 35 scheduled appointments TOTAL scheduled appointments for FY2013 1178 In addition, the Flow Core continued to maintain CLIA accreditation in support of immunophenotyping and protein expression studies used in NHGRI clinical research protocols.
国家人类基因组研究所的流式细胞术核心设施的目标是为所有NHGRI研究人员提供高质量的流式细胞术服务。核心的作用是提高研究所进行的科学研究的范围和质量。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

DAVID M. BODINE其他文献

DAVID M. BODINE的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('DAVID M. BODINE', 18)}}的其他基金

VISION: ValIdated Systematic IntegratiON of epigenomic data
愿景:经过验证的表观基因组数据的系统整合
  • 批准号:
    9183143
  • 财政年份:
    2016
  • 资助金额:
    $ 80.59万
  • 项目类别:
VISION: ValIdated Systematic IntegratiON of epigenomic data
愿景:经过验证的表观基因组数据的系统整合
  • 批准号:
    9976999
  • 财政年份:
    2016
  • 资助金额:
    $ 80.59万
  • 项目类别:
Global Predictions and Tests of Hematopoietic Regulation
造血调节的整体预测和测试
  • 批准号:
    8912612
  • 财政年份:
    2004
  • 资助金额:
    $ 80.59万
  • 项目类别:
ENHANCER ELEMENTS IN THE HUMAN B GLOBIN GENE CLUSTER
人类 B 珠蛋白基因簇中的增强子元件
  • 批准号:
    3049744
  • 财政年份:
    1986
  • 资助金额:
    $ 80.59万
  • 项目类别:
ENHANCER ELEMENTS IN THE HUMAN B GLOBIN GENE CLUSTER
人类 B 珠蛋白基因簇中的增强子元件
  • 批准号:
    3049745
  • 财政年份:
    1986
  • 资助金额:
    $ 80.59万
  • 项目类别:
ENHANCER ELEMENTS IN THE HUMAN B GLOBIN GENE CLUSTER
人类 B 珠蛋白基因簇中的增强子元件
  • 批准号:
    3049743
  • 财政年份:
    1985
  • 资助金额:
    $ 80.59万
  • 项目类别:
ENHANCER ELEMENTS IN THE HUMAN B GLOBIN GENE CLUSTER
人类 B 珠蛋白基因簇中的增强子元件
  • 批准号:
    3049742
  • 财政年份:
    1985
  • 资助金额:
    $ 80.59万
  • 项目类别:
Improving gene transfer to provide intracellular immuniz
改善基因转移以提供细胞内免疫
  • 批准号:
    6988880
  • 财政年份:
  • 资助金额:
    $ 80.59万
  • 项目类别:
HEMATOPOIETIC STEM CELL BIOLOGY
造血干细胞生物学
  • 批准号:
    6681484
  • 财政年份:
  • 资助金额:
    $ 80.59万
  • 项目类别:
Hematopoietic Stem Cell Biology
造血干细胞生物学
  • 批准号:
    8565520
  • 财政年份:
  • 资助金额:
    $ 80.59万
  • 项目类别:

相似海外基金

Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
  • 批准号:
    MR/S03398X/2
  • 财政年份:
    2024
  • 资助金额:
    $ 80.59万
  • 项目类别:
    Fellowship
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
  • 批准号:
    2338423
  • 财政年份:
    2024
  • 资助金额:
    $ 80.59万
  • 项目类别:
    Continuing Grant
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
  • 批准号:
    EP/Y001486/1
  • 财政年份:
    2024
  • 资助金额:
    $ 80.59万
  • 项目类别:
    Research Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
  • 批准号:
    MR/X03657X/1
  • 财政年份:
    2024
  • 资助金额:
    $ 80.59万
  • 项目类别:
    Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
  • 批准号:
    2348066
  • 财政年份:
    2024
  • 资助金额:
    $ 80.59万
  • 项目类别:
    Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
  • 批准号:
    AH/Z505481/1
  • 财政年份:
    2024
  • 资助金额:
    $ 80.59万
  • 项目类别:
    Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
  • 批准号:
    10107647
  • 财政年份:
    2024
  • 资助金额:
    $ 80.59万
  • 项目类别:
    EU-Funded
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
  • 批准号:
    2341402
  • 财政年份:
    2024
  • 资助金额:
    $ 80.59万
  • 项目类别:
    Standard Grant
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
  • 批准号:
    10106221
  • 财政年份:
    2024
  • 资助金额:
    $ 80.59万
  • 项目类别:
    EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
  • 批准号:
    AH/Z505341/1
  • 财政年份:
    2024
  • 资助金额:
    $ 80.59万
  • 项目类别:
    Research Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了