NHGRI/DIR Flow Cytometry Core

NHGRI/DIR 流式细胞术核心

• 批准号: 10672091
• 负责人: DAVID M. BODINE
• 金额: $ 142.35万
• 依托单位: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10672091
• 关键词: Animals; Blood Cells; Cell Nucleus; Cell Separation; Cell Size; Cells; Color; Development; Enhancers; Equipment; Flow Cytometry; Gene Expression Profile; Genes; Genetic Transcription; Hematopoiesis; Human; Human Resources; Lasers; Measures; Mus; National Human Genome Research Institute; Neurons; Population; Property; Research Personnel; Running; Sampling; Services; Sorting; Surface; Suspensions; Technology; Tissues; Training; Transplantation; Zebrafish; base; cell type; design; experimental study; high throughput screening; indexing; instrument; magnetic cell separation; particle; single-cell RNA sequencing; statistics

The Flow Cytometry Core provides NHGRI researchers access to high-quality flow cytometry services. Flow cytometry can be used to analyze, identify, and isolate subpopulations of cells from mixed populations and to classify cells that may represent less than 0.1% of the total sample. This technology can be used to analyze any cell type as well as many subcellular particles that can be prepared as a single-cell suspension. Multiple parameters on thousands of cells per second can be measured simultaneously, including cell size, cell complexity, and surface and intracellular markers. Current projects include isolation of human neuronal nuclei for gene profile expression studies, many zebrafish hematopoiesis projects as well as active involvement in a variety of applications including the isolation of tissue and blood cell populations for animal transplantation experiments; isolation of cell sub-populations for analyses of functional cellular properties and gene transcription profiles, as well as high throughput screening of enhancer regions. The index-sorting feature available on our sorters enables investigators to sort cells into single wells of plates, and easily identify them on plots, in statistics views, and in a detailed results file. These features allow the rapid isolation and characterization of single cells for use in single cell RNA-seq. The Core is equipped with one BD FACS ARIA Fusion enclosed in a biosafety cabinet - five laser, 21 color instrument, one BDFACSAria; one SONY SH800 chip based benchtop sorter in a biosafety cabinet capable of six color analysis one thirteen color Beckman Coulter CyotFLEX S analyzer, one 21 color Beckman Coulter CytoFLEX LX analyzer; a Miltenyi Auto MACS for magnetic cell separation and a HESKA HT5 for murine CBC/Differential analysis. Core personnel are available for training, development, and project execution. The Flow Cytometry Core is currently staffed by Stacie Anderson (Core Director,100% effort, GMBB), and Martha Kirby (Associate Director, 100% effort, GMBB). These investigators run the sorters, maintain the analyzers and other equipment and provide expert advice on design and execution of experiments.

流式细胞术核心为NHGRI的研究人员提供了高质量的流式细胞术服务。流式细胞术可用于分析、鉴定和从混合群体中分离细胞亚群，并对可能占总样本不到0.1%的细胞进行分类。该技术可用于分析任何细胞类型以及许多可制备为单细胞悬浮液的亚细胞颗粒。每秒可以同时测量数千个细胞的多个参数，包括细胞大小、细胞复杂性、表面和细胞内标记物。目前的项目包括分离人类神经元核用于基因谱表达研究，许多斑马鱼造血项目以及积极参与各种应用，包括分离组织和血细胞群体用于动物移植实验；分离细胞亚群，用于分析细胞功能特性和基因转录谱，以及高通量筛选增强子区域。我们的分选器上提供的索引排序功能使调查人员能够将细胞分类到单孔板中，并在图中，统计视图和详细的结果文件中轻松识别它们。这些特点允许快速分离和表征单细胞用于单细胞RNA-seq。