Acquisition of a Yokogawa CSU-W1 Spinning Disk Confocal Microscopy System

描述（由申请人提供）：要求资金用于购买横河CSU-W1旋转盘共聚焦系统，该系统与带有Andor EM CCD探测器的尼康Eclipse NI-E直立显微镜耦合。该仪器将取代1995年购买的Noran/Prairie Technology扫描共聚焦显微镜。新仪器将被安置在由NIGMS/NCRR生物医学卓越研究中心（COBRE）神经科学资助（P30 RR032135/P30 GM103498, 7/1/2011-6/30/2016）支持的神经科学成像/生理学核心设施中。神经科学COBRE成像/生理学核心由一位训练有素的全职显微镜专家管理。横河CSU W-1是一个现代化的、模块化的、积极支持的系统，收购它将极大地增强Core的实时成像能力，为医学院和弗吉尼亚大学校园其他学院的研究人员提供高速、高分辨率的显微镜资源。这些研究人员经常记录快速的、局部的细胞内事件的光学测量，例如活细胞和组织中的钙瞬变。横河旋转盘将显著提高核心的能力，以满足当前核心研究人员的新需求，同时为额外的资助和新的研究人员提供机会，以扩大他们的研究计划的范围。横河系统将使用直立而非倒置的显微镜平台，通过采用高数值孔径生理浸透镜，可以更好地观察完整的薄组织，如孤立的血管或肠道、膀胱或尿路上皮条。横河系统可以同时记录两个低光信号与双512x512 EM CCD相机在52 fps（帧每秒），速率远高于我们的其他仪器。更高的分辨率（2560X2160）成像较亮的样品可以实现与Zyla sCMOS相机在100 fps。这些相机为我们的多用户设施中遇到的多种样本类型提供了极大的灵活性。此外，横河系统将有多条激光线，通过允许同时测量来自特定细胞的多个荧光信号，大大扩大了其实验能力。COBRE成像/生理学核心在支持实时成像研究和出版方面有着悠久的历史，但其用于高速采集的仪器已经过时，无法再满足当前的研究需求。

DESCRIPTION (provided by applicant): Funds are requested to purchase a Yokogawa CSU-W1 spinning disk confocal system coupled to a Nikon Eclipse NI-E upright microscope with Andor EM CCD detectors. This instrument will replace a Noran/Prairie Technology Scanning confocal microscope, which was purchased in 1995. The new instrument will be housed in the Neuroscience Imaging/Physiology Core Facility supported by the NIGMS/NCRR Center of Biomedical Research Excellence (COBRE) in Neuroscience grant (P30 RR032135/P30 GM103498, 7/1/2011-6/30/2016). The Neuroscience COBRE Imaging/Physiology Core is administered by a full-time, highly trained microscopist. Acquisition of the Yokogawa CSU W-1, a modern, modular, and actively supported system, would greatly enhance the live imaging capabilities of the Core, expanding the high-speed, high-resolution microscopy resources available to investigators within the College of Medicine and in other Colleges on the UVM campus. These investigators routinely record optical measurements of fast, localized intracellular events, such as calcium transients from living cells and tissues. The Yokogawa spinning disk will significantly improve the capabilities of the Core to meet the emerging needs of the present Core investigators, while providing the opportunity for additional funded and new investigators to expand the scope of their research programs. The Yokogawa system will use an upright rather an inverted microscope platform, allowing better access to intact thin tissues like isolated vessels or gut, bladder or urothelial strips by employing high numerical aperture physiology dipping lenses. The Yokogawa system can simultaneously record two low light signals with dual 512x512 EM CCD cameras at 52 fps (frames per second), a rate well above our other instrumentation. Higher resolution (2560X2160) imaging for brighter samples can be achieved with the Zyla sCMOS camera at 100 fps. These cameras allow great flexibility for the multiple sample types encountered in our multi-user facility. In addition, the Yokogawa system will have multiple laser lines, greatly broadening its experimental capacity by allowing simultaneous measurements of multiple fluorescent signals from defined cells. The COBRE Imaging/Physiology Core has a long history of supporting live imaging research and publication, but its instrumentation for high speed acquisition is obsolete, and can no longer meet current research demands.