Acquisition of a Yokogawa CSU-W1 Spinning Disk Confocal Microscopy System

购买横河电机 CSU-W1 转盘共焦显微镜系统

• 批准号: 8635090
• 负责人: Rodney L Parsons
• 金额: $ 51.87万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-15 至 2015-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8635090
• 关键词: Bladder; Calcium; Cancer Grant Supplements (P30); Cells; Centers of Research Excellence; Confocal Microscopy; Core Facility; Coupled; Event; Funding; Funding Applicant; Housing; Image; Lasers; Life; Light; Measurement; Medicine; Microscope; Microscopy; National Center for Research Resources; National Institute of General Medical Sciences; Neurosciences; Optics; Physiology; Publications; Recording of previous events; Research; Research Personnel; Resolution; Resources; Sampling; Scanning; Signal Transduction; Speed; Support System; System; Technology; Time; Tissues; Training; charge coupled device camera; college; detector; flexibility; improved; instrument; instrumentation; lens; meetings; programs; ranpirnase

DESCRIPTION (provided by applicant): Funds are requested to purchase a Yokogawa CSU-W1 spinning disk confocal system coupled to a Nikon Eclipse NI-E upright microscope with Andor EM CCD detectors. This instrument will replace a Noran/Prairie Technology Scanning confocal microscope, which was purchased in 1995. The new instrument will be housed in the Neuroscience Imaging/Physiology Core Facility supported by the NIGMS/NCRR Center of Biomedical Research Excellence (COBRE) in Neuroscience grant (P30 RR032135/P30 GM103498, 7/1/2011-6/30/2016). The Neuroscience COBRE Imaging/Physiology Core is administered by a full-time, highly trained microscopist. Acquisition of the Yokogawa CSU W-1, a modern, modular, and actively supported system, would greatly enhance the live imaging capabilities of the Core, expanding the high-speed, high-resolution microscopy resources available to investigators within the College of Medicine and in other Colleges on the UVM campus. These investigators routinely record optical measurements of fast, localized intracellular events, such as calcium transients from living cells and tissues. The Yokogawa spinning disk will significantly improve the capabilities of the Core to meet the emerging needs of the present Core investigators, while providing the opportunity for additional funded and new investigators to expand the scope of their research programs. The Yokogawa system will use an upright rather an inverted microscope platform, allowing better access to intact thin tissues like isolated vessels or gut, bladder or urothelial strips by employing high numerical aperture physiology dipping lenses. The Yokogawa system can simultaneously record two low light signals with dual 512x512 EM CCD cameras at 52 fps (frames per second), a rate well above our other instrumentation. Higher resolution (2560X2160) imaging for brighter samples can be achieved with the Zyla sCMOS camera at 100 fps. These cameras allow great flexibility for the multiple sample types encountered in our multi-user facility. In addition, the Yokogawa system will have multiple laser lines, greatly broadening its experimental capacity by allowing simultaneous measurements of multiple fluorescent signals from defined cells. The COBRE Imaging/Physiology Core has a long history of supporting live imaging research and publication, but its instrumentation for high speed acquisition is obsolete, and can no longer meet current research demands.

描述（由申请人提供）： 申请资金购买横河 CSU-W1 转盘共焦系统，该系统与带有 Andor EM CCD 探测器的 Nikon Eclipse NI-E 正置显微镜相结合。该仪器将取代 1995 年购买的 Noran/Prairie Technology 扫描共焦显微镜。新仪器将安装在神经科学成像/生理学核心设施中，该设施由 NIGMS/NCRR 生物医学研究卓越中心 (COBRE) 神经科学补助金 (P30 RR032135/P30 GM103498， 2011年7月1日至2016年6月30日）。神经科学 COBRE 成像/生理学核心由训练有素的全职显微镜师管理。收购横河电机CSU W-1是一种现代化、模块化和主动支持的系统，将大大增强核心的实时成像能力，扩大医学院和UVM校园其他学院的研究人员可用的高速、高分辨率显微镜资源。这些研究人员定期记录快速、局部细胞内事件的光学测量结果，例如活细胞和组织中的钙瞬变。横河电机旋转盘将显着提高核心的能力，以满足当前核心研究人员的新需求，同时为额外资助的新研究人员提供扩大其研究项目范围的机会。横河系统将使用直立而不是倒置的显微镜平台，通过采用高数值孔径生理学浸渍透镜，可以更好地观察完整的薄组织，如孤立的血管或肠道、膀胱或尿路上皮条。横河系统可以使用双 512x512 EM CCD 相机以 52 fps（每秒帧数）同时记录两个弱光信号，该速率远高于我们的其他仪器。使用 Zyla sCMOS 相机可以以 100 fps 的速度实现更高分辨率 (2560X2160) 的成像，从而获得更亮的样品。这些相机为我们的多用户设施中遇到的多种样品类型提供了极大的灵活性。此外，横河系统将拥有多条激光线，通过允许同时测量来自特定细胞的多个荧光信号，极大地扩大了其实验能力。 COBRE 成像/生理学核心在支持实时成像研究和出版方面有着悠久的历史，但其高速采集仪器已经过时，无法满足当前的研究需求。