Acquisition of a Zeiss LSM 7 MP multiphoton microscope

购买 Zeiss LSM 7 MP 多光子显微镜

• 批准号: 8245457
• 负责人: Rodney L Parsons
• 金额: $ 59.85万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8245457
• 关键词: Blood Vessels; Brain; Calcium; Cells; Centers of Research Excellence; Cerebrum; Communication; Computer software; Core Facility; Coupling; Endothelium; Extracellular Space; Funding; Funding Applicant; Glutamates; Grant; Housing; Image; Lasers; Life; Location; Microscope; Modeling; Monitor; National Center for Research Resources; Neurons; Neurosciences; Noise; Penetration; Phase; Photons; Physiologic pulse; Physiology; Research; Research Personnel; Research Project Grants; Sapphire; Smooth Muscle; Speed; Structure; Support System; System; Time; Tissues; Training; arteriole; cell type; innovation; instrument; interest; meetings; photolysis; programs; tool; two-photon

DESCRIPTION (provided by applicant): Funds are requested to purchase a Zeiss LSM MP multiphoton microscope with a Coherent Chameleon Ultra II Ti:Sapphire pulsed laser. This instrument will replace our current BioRad Radiance Multiphoton Microscope, which no longer meets the needs of our users. In addition, Carl Zeiss is phasing out their legacy BioRad systems and no longer stocks replacement hardware or provides software upgrades for the Radiance 2300 system. The dedicated multiphoton microscope will be housed in the Imaging/Physiology Core Facility supported by the NCRR Center of Biomedical Research Excellence (COBRE) in Neuroscience grant (5P20 RR16435, 07/01/06 - 06/30/11). The competitive renewal of the COBRE grant is expected to start 7/1/01/2011 and provide 5 additional years of Core support. The Neuroscience COBRE Imaging/Physiology Core is administered by a full-time, highly trained microscopist. Acquisition of the Zeiss LSM 7 MP dedicated multiphoton microscope, a modern, modular, and actively supported system, would greatly enhance the capabilities of the Core and would meet the emerging needs of the present Core investigators and provide the opportunity for additional funded investigators to significantly expand the scope of their research programs. The new Zeiss multiphoton microscope, model LSM 7MP, offers impressively rapid acquisition speed, about 4 times higher than the speed of our BioRad Radiance 2100 MPD two-photon microscope. The high acquisition speed combined with the superior sensitivity and lower noise levels makes LSM 7 MP perfect for studying the activity of living neurons and other cell types in the brain. In recent years, multiphoton photolysis of caged compounds (uncaging) is increasingly used as a tool to quickly deliver calcium and other substances intracellularly or in the extracellular space with great precision in time, location and volume. Furthermore, multiphoton uncaging allows deeper penetration into live tissues, with smaller volumes compared to single photon photolysis. Our highly innovative research projects that investigate the neuro-vascular coupling in the brain and cerebral arteriole smooth muscle and endothelium function absolutely require the use of photolysis of caged calcium, IP3, glutamate and ATP. The Zeiss LSM 7 MP is capable of two photon photolysis localized within multiple arbitrarily defined regions of interest without slowing down the acquisition speed. This will allow us to simultaneously uncage and image living cells or tissue with a speed that is adequate for monitoring and recording the changes in cellular activity that occur as a result of uncaging. Moreover, this will enable us to simultaneously or consecutively uncage compounds in more than one cell or location thus providing us with the unique opportunity to study the interactions and communications between different cells or structures in the brain. PUBLIC HEALTH RELEVANCE: This instrument would support research designed to understand the development and normal functioning of the brain and autonomic organs and their alteration in response to disease. Imaging of cellular responses within brain slices and organs will provide key insight into the regulation of neuronal, vascular, and connective tissue function and their interdependence. This insight will inform development of therapeutic approaches for diseases that strike these tissues.

描述（由申请人提供）：需要资金购买配备 Coherent Chameleon Ultra II Ti:Sapphire 脉冲激光器的 Zeiss LSM MP 多光子显微镜。该仪器将取代我们当前的 BioRad Radiance 多光子显微镜，后者已不能满足用户的需求。此外，Carl Zeiss 正在逐步淘汰其旧版 BioRad 系统，并且不再库存替换硬件或为 Radiance 2300 系统提供软件升级。专用多光子显微镜将安装在由 NCRR 生物医学卓越研究中心 (COBRE) 神经科学资助 (5P20 RR16435，2006 年 7 月 1 日 - 2011 年 6 月 30 日) 支持的成像/生理学核心设施中。 COBRE 赠款的竞争性更新预计将于 2011 年 1 月 7 日开始，并提供额外 5 年的核心支持。神经科学 COBRE 成像/生理学核心由训练有素的全职显微镜师管理。采购 Zeiss LSM 7 MP 专用多光子显微镜（一种现代化、模块化和主动支持的系统）将大大增强核心的能力，满足当前核心研究人员的新需求，并为其他受资助的研究人员提供显着扩大研究范围的机会 程序。新型蔡司多光子显微镜 LSM 7MP 提供令人印象深刻的快速采集速度，大约比我们的 BioRad Radiance 2100 MPD 双光子显微镜的速度高 4 倍。高采集速度与卓越的灵敏度和较低的噪声水平相结合，使 LSM 7 MP 非常适合研究大脑中活体神经元和其他细胞类型的活动。近年来，笼状化合物的多光子光解（解笼）越来越多地用作一种工具，以在时间、位置和体积上非常精确地快速将钙和其他物质递送到细胞内或细胞外空间。此外，多光子解禁允许更深入地渗透到活组织中，与单光子光解相比，体积更小。我们高度创新的研究项目研究大脑和脑小动脉平滑肌和内皮功能中的神经血管耦合，绝对需要使用笼中钙、IP3、谷氨酸和 ATP 的光解作用。 Zeiss LSM 7 MP 能够在多个任意定义的感兴趣区域内进行两个光子光解，而不会降低采集速度。这将使我们能够同时释放和成像活细胞或组织，其速度足以监测和记录由于释放而发生的细胞活动的变化。此外，这将使我们能够同时或连续地释放多个细胞或位置中的化合物，从而为我们提供独特的机会来研究大脑中不同细胞或结构之间的相互作用和通信。 公共健康相关性：该工具将支持旨在了解大脑和自主器官的发育和正常功能及其对疾病反应的变化的研究。脑切片和器官内细胞反应的成像将为神经元、血管和结缔组织功能的调节及其相互依赖性提供重要的见解。这一见解将为针对这些组织的疾病的治疗方法的开发提供信息。