Cryopreservation Research/Development

低温保存研究/开发

• 批准号: 8844938
• 负责人: DAVID HEIMBROOK
• 金额: $ 0.69万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-26 至 2018-09-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8844938
• 关键词: Area; Back; Cryopreservation; Development; Disasters; Embryo; Excision; Freezing; Genetic; Genetic Engineering; Genotype; Inbreeding; Laboratories; Liquid substance; Mouse Strains; National Cancer Institute; Nitrogen; Ovary; Services; Staging; Techniques; Vial device; anticancer research; cost effective; cryobiology; mutant; programs; repository; research and development; sperm cell

The Cryopreservation R&D program area offers services for banking unique strains of mice by preserving their frozen germplasm in liquid nitrogen (-196¿ C). Cryopreservation provides a cost-effective alternative to maintaining strains no longer in use by allowing their removal from the shelf storage. Additionally, the laboratory has a storage capacity of 44,000 vials and as a back-up against any imponderable disaster; a number of vials from each strain are kept at the National Cancer Institute-Frederick Central Repository. To further cancer research, the Cryobiology Laboratory utilizes state-of-the-art techniques that make it possible to offer different options for preserving a given mouse strain. Such techniques include freezing embryos at different developmental stages, freezing ovaries, and freezing sperm. With these options we are able to cryopreserve most inbred, spontaneous mutant, or genetically engineered strains, while maintaining the genetic background and genotype.

冷冻保存研发项目区通过在液氮（-196 ℃）中保存其冷冻种质，为独特的小鼠品系提供银行服务。冷冻保存提供了一种经济有效的替代方法，通过允许将其从货架储存中取出来维持不再使用的菌株。此外，该实验室拥有44，000个小瓶的存储容量，并作为任何无法估量的灾难的备份;每个菌株的一些小瓶保存在国家癌症研究所-弗雷德里克中央储存库。为了进一步开展癌症研究，低温生物学实验室利用最先进的技术，为保存给定的小鼠品系提供不同的选择。这些技术包括冷冻不同发育阶段的胚胎，冷冻卵巢和冷冻精子。通过这些选择，我们能够冷冻保存大多数近交系、自发突变体或遗传工程菌株，同时保持遗传背景和基因型。