Host and symbiont determinants of colonization by a co-evolved gut community
共同进化的肠道群落定植的宿主和共生体决定因素
基本信息
- 批准号:8603715
- 负责人:
- 金额:$ 34.76万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-01-02 至 2017-12-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectAnimalsApidaeApisBacteriaBacterial GenesBeesBiologyCharacteristicsCommunitiesComplexDevelopmentDrosophila genusEnabling FactorsEnvironmentFoodFosteringFoundationsGenesGeneticGenomeGenotypeHealthHigh-Throughput Nucleotide SequencingHindgutHoneyHumanHuman DevelopmentIndividualInsectaIntegration Host FactorsLaboratoriesLeadMammalsMediatingMetabolicMethodsMicrobeModelingMonitorMutagenesisMutationNomenclaturePathway interactionsProcessProtocols documentationPublishingRNA InterferenceResourcesSpecificitySystemWorkaxenic culturebasedisease characteristicgenome sequencinggenome-widegut microbiotaileummembermicrobial communitymutantpathogenic bacteriapublic health relevanceresponsescreeningtooltranscriptome sequencing
项目摘要
Host and symbiont determinants of colonization by a co-evolved gut community
Project Summary
Host-specific gut bacteria are central to the biology and health of animals, but elucidating the
processes that govern normal and atypical assembly of gut communities is challenging. Most gut
communities, and specifically those of humans, are dauntingly complex; whereas others, such as
that of Drosophila, have variable compositions dominated by opportunistic bacterial species from
other environments. In the honey bee (Apis mellifera), the ileum region of the hindgut contains a
dense but simple community dominated by only three specialized bacterial species that comprise
>95% of bacteria despite the continual entry of diverse environmental microbes present in food.
These three species are found only in bee guts, and distinct but related strains are found in related
bee species. Our pilot work established axenic culture conditions, official nomenclature, and
reference strains for all bee gut community members, transposon-based mutagenesis methods,
protocols for controlling colonization with specific isolates or mutants, and methods for quantitative
description of community composition. Furthermore, the host is amenable to genetic studies:
sequenced genome and associated resources are available for the honey bee, and RNAi methods
have been established. Building on these foundations, we will use the honey bee ileum as a model
gut community to determine the specific bacterial and host factors that underlie the establishment
of a persistent gut community characteristic of a host species. We will use expression analyses
and genome-wide mutagenesis and mutant screening based on high-throughput sequencing to
identify symbiont genes affecting colonization of the bee ileum and to elucidate the effects of host
genes and pathways on colonization. We will examine how a focal pioneer symbiont influences the
assembly and subsequent composition of the gut community. This will be achieved by monitoring
succession in experimental gut communities and by identifying symbiont genes that affect
colonization by other bacterial species and strains, including both the symbionts normally present
in the community and the opportunistic or pathogenic bacteria that are typically excluded. These
results will reveal host and symbiont-based processes that govern development of a normal, host-
specific gut microbiota and will illuminate how and why gut communities sometimes develop
abnormally, to the detriment of host health.
!
共同进化的肠道群落定殖的宿主和共生体决定因素
项目摘要
宿主特异性肠道细菌对动物的生物学和健康至关重要,但阐明宿主特异性肠道细菌对动物的生物学和健康至关重要。
控制肠道群落的正常和非典型组装的过程具有挑战性。最有胆量
社区,特别是人类的社区,是令人生畏的复杂;而其他社区,如
果蝇的,具有可变的组成,由机会细菌物种占主导地位,
其他环境。在蜜蜂(Apis mellifera)中,后肠的回肠区域含有一个
密集而简单的群落,只有三种专门的细菌物种,
尽管食物中存在的各种环境微生物不断进入,但细菌仍占95%以上。
这三个物种只在蜜蜂的内脏中发现,不同但相关的菌株在相关的
蜜蜂种类我们的试点工作建立了无菌培养条件,官方命名,
所有蜜蜂肠道群落成员的参考菌株,基于转座子的诱变方法,
控制特定分离物或突变体定殖的方案,以及定量测定
社区组成的描述。此外,宿主适合遗传研究:
已测序的基因组和相关资源可用于蜜蜂,
已经建立。在这些基础上,我们将使用蜜蜂回肠作为模型
肠道菌群,以确定建立的特定细菌和宿主因素
一种宿主物种特有的持久性肠道群落。我们将使用表情分析
以及基于高通量测序的全基因组诱变和突变体筛选,
鉴定影响蜜蜂回肠定殖的共生体基因,并阐明宿主
基因和途径的影响。我们将研究一个焦点先锋共生体如何影响
组装和随后的肠道群落组成。这将通过监测
实验肠道群落的演替,并通过识别影响
其他细菌种属和菌株的定植,包括通常存在的共生体
以及通常被排除在外的机会性或致病性细菌。这些
结果将揭示主机和共生体为基础的过程,支配发展的正常,主机-
特定的肠道微生物群,并将阐明如何以及为什么肠道社区有时发展
不正常地,损害宿主的健康。
!
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Nancy A. Moran其他文献
Correction to: The genome sequence of the grape phylloxera provides insights into the evolution, adaptation, and invasion routes of an iconic pest
- DOI:
10.1186/s12915-020-00864-7 - 发表时间:
2020-09-11 - 期刊:
- 影响因子:4.500
- 作者:
Claude Rispe;Fabrice Legeai;Paul D. Nabity;Rosa Fernández;Arinder K. Arora;Patrice Baa-Puyoulet;Celeste R. Banfill;Leticia Bao;Miquel Barberà;Maryem Bouallègue;Anthony Bretaudeau;Jennifer A. Brisson;Federica Calevro;Pierre Capy;Olivier Catrice;Thomas Chertemps;Carole Couture;Laurent Delière;Angela E. Douglas;Keith Dufault-Thompson;Paula Escuer;Honglin Feng;Astrid Forneck;Toni Gabaldón;Roderic Guigó;Frédérique Hilliou;Silvia Hinojosa-Alvarez;Yi-min Hsiao;Sylvie Hudaverdian;Emmanuelle Jacquin-Joly;Edward B. James;Spencer Johnston;Benjamin Joubard;Gaëlle Le Goff;Gaël Le Trionnaire;Pablo Librado;Shanlin Liu;Eric Lombaert;Hsiao-ling Lu;Martine Maïbèche;Mohamed Makni;Marina Marcet-Houben;David Martínez-Torres;Camille Meslin;Nicolas Montagné;Nancy A. Moran;Daciana Papura;Nicolas Parisot;Yvan Rahbé;Mélanie Ribeiro Lopes;Aida Ripoll-Cladellas;Stéphanie Robin;Céline Roques;Pascale Roux;Julio Rozas;Alejandro Sánchez-Gracia;Jose F. Sánchez-Herrero;Didac Santesmasses;Iris Scatoni;Rémy-Félix Serre;Ming Tang;Wenhua Tian;Paul A. Umina;Manuella van Munster;Carole Vincent-Monégat;Joshua Wemmer;Alex C. C. Wilson;Ying Zhang;Chaoyang Zhao;Jing Zhao;Serena Zhao;Xin Zhou;François Delmotte;Denis Tagu - 通讯作者:
Denis Tagu
Two gut community enterotypes recurin diverse bumblebee species
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:
- 作者:
Li Jilian;J. Elijah Powell;Guo Jun;Jay D. Evans;Wu Jie;Paul Williams;Lin Qinhui;Nancy A. Moran;Zhang Zhigang - 通讯作者:
Zhang Zhigang
Weitere Übersichtsartikel aus dem Gesamtgebiet der Genetik schließen sich an
遗传疾病的整体研究
- DOI:
- 发表时间:
2010 - 期刊:
- 影响因子:0
- 作者:
J. Lederberg;Thomas C. Rindfleisch;Leonard A. Herzenberg;Xin Zhao;James R. Broach;N. Kröger;Kent W. Hunter;N. P. Crawford;Stephen P. Goff;Nancy A. Moran;John P. McCutcheon;Eduardo P. C. Rocha;M. Surette;Transposon Tn;Fachgebiet der Genetik;S. Redlhammer - 通讯作者:
S. Redlhammer
Glyphosate Effects on Growth and Biofilm Formation in Bee Gut Symbionts and Diverse Associated Bacteria
草甘膦对蜂肠共生体和多种相关细菌生长和生物膜形成的影响
- DOI:
10.1101/2024.03.20.585985 - 发表时间:
2024 - 期刊:
- 影响因子:0
- 作者:
Erick V. S. Motta;Tyler K. de Jong;Alejandra Gage;Joseph A. Edwards;Nancy A. Moran - 通讯作者:
Nancy A. Moran
Intracellular defensive symbiont is culturable and capable of transovarial, vertical transmission
细胞内防御共生体是可培养的并且能够跨卵巢、垂直传播
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:0
- 作者:
Gerald P. Maeda;Mary Katherine Kelly;Aadhunik Sundar;Nancy A. Moran - 通讯作者:
Nancy A. Moran
Nancy A. Moran的其他文献
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{{ truncateString('Nancy A. Moran', 18)}}的其他基金
Elucidating the molecular bases of species interactions in host-associated bacterial communities
阐明宿主相关细菌群落中物种相互作用的分子基础
- 批准号:
10165745 - 财政年份:2019
- 资助金额:
$ 34.76万 - 项目类别:
Elucidating the molecular bases of species interactions in host-associated bacterial communities
阐明宿主相关细菌群落中物种相互作用的分子基础
- 批准号:
10620345 - 财政年份:2019
- 资助金额:
$ 34.76万 - 项目类别:
Elucidating the molecular bases of species interactions in host-associated bacterial communities
阐明宿主相关细菌群落中物种相互作用的分子基础
- 批准号:
10403669 - 财政年份:2019
- 资助金额:
$ 34.76万 - 项目类别:
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