Elucidating the molecular bases of species interactions in host-associated bacterial communities

阐明宿主相关细菌群落中物种相互作用的分子基础

基本信息

  • 批准号:
    10403669
  • 负责人:
  • 金额:
    $ 39.13万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-06-15 至 2024-05-31
  • 项目状态:
    已结题

项目摘要

Project Summary/Abstract Microbial communities exert major impacts on animal biology and human health, and disruption of these communities is associated with multiple disease states. To date, the field of microbiome research has been dominated by surveys of microbial community compositions and by analyses correlating composition with host phenotypes. But there have been few attempts to directly link the specific, causal processes that determine colonization dynamics and success of host-associated bacteria, and how these interactions ultimately affect hosts. The proposed research plan is motivated by the need for experimental systems to identify the mechanisms that control the composition and consequences of host-associated bacterial communities. This work focuses on two model systems that provide complementary approaches to examining host-associated communities, and that offer new opportunities to identify the mechanisms underlying host colonization. The honey bee and its specialized gut microbiota provides an exceptional model for multispecies gut communities as it shares many features with the human gut microbiota. In both human and bee guts, a stable, healthy community bestows “colonization resistance”, the exclusion of foreign microorganisms; in both systems, disruption can result in dysbiosis and expansion of atypical communities, including enteric pathogens. The human system is highly complex and not amenable to experiments, but, for the bee gut, we are able to culture isolates of all component bacterial species and to introduce these to microbiota-free hosts to establish defined communities. We have already developed genetic tools for experimental manipulation of the dominant species. One set of experiments will identify the direct host-bacterial interactions that determine colonization success or failure of particular strains that vary in ability to colonize honey bees. Existing results from a mutagenesis screen indicate that features of the outer cell envelope play essential roles during host colonization, and we will use new genetic tools to determine which of these factors are key to acceptance by hosts. In addition to elucidating the mechanisms that enable specific bacterial strains to mono-colonize specific hosts, a second set of experiments will investigate how the interactions between microbial strains, which range from metabolic co-dependency to direct toxin-mediated antagonism, determine community membership. The pea aphid and its endosymbionts provide an effective model for how intracellular bacterial associates stably colonize host cells. Our newly devised techniques allow isolation, manipulation and inter-host transfer of endosymbionts. To address how hosts control endosymbiont replication and persistence, we will perform genomic comparisons, biochemical experiments to test effects of host-produced gene products on endosymbiont cells, and physical and structural characterization of endosymbiont outer membrane proteins. Through focus on experimental models that provide tractable examples of host-associated bacteria, this work will illuminate the mechanisms that underlie ability of bacterial symbionts to colonize hosts.
项目总结/摘要 微生物群落对动物生物学和人类健康产生重大影响, 社区与多种疾病状态相关。到目前为止,微生物组研究领域已经 以微生物群落组成调查和分析与宿主相关的组成为主 表型但是,很少有人试图将决定性的具体因果过程直接联系起来, 宿主相关细菌的定植动力学和成功,以及这些相互作用最终如何影响 hosts.拟议的研究计划的动机是需要实验系统,以确定 控制宿主相关细菌群落的组成和后果的机制。这 工作集中在两个模型系统,提供互补的方法来检查主机相关的 社区,并提供新的机会,以确定潜在的主机殖民化的机制。 蜜蜂及其专门的肠道微生物群为多物种肠道提供了一个特殊的模型 因为它与人类肠道微生物群有许多共同特征。在人类和蜜蜂的肠道中, 健康的社区赋予“殖民抵抗力”,排除外来微生物;在这两个 如果系统受到破坏,可能导致生态失调和非典型群落的扩大,包括肠道病原体。 人类的系统非常复杂,不适合实验,但是,对于蜜蜂的肠道,我们能够 培养所有组成细菌物种的分离株,并将其引入无微生物群的宿主, 定义的社区。我们已经开发出了遗传工具,用于实验性地操纵占优势的 物种一组实验将确定决定定植的宿主-细菌直接相互作用 成功或失败的特定菌株,不同的能力殖民蜜蜂。现有成果来自 诱变筛选表明,外细胞被膜特征在寄主过程中起重要作用 殖民化,我们将使用新的遗传工具来确定这些因素中的哪些是接受的关键。 hosts.除了阐明使特定细菌菌株能够单定殖特定细菌的机制之外, 宿主,第二组实验将研究微生物菌株之间如何相互作用,其范围 从代谢共依赖性到直接毒素介导的拮抗作用,确定群落成员。 豌豆蚜及其内共生菌为研究胞内细菌如何缔合提供了一个有效的模型 稳定地定殖宿主细胞。我们新设计的技术允许分离,操纵和宿主间转移 内共生体为了解决主机如何控制内共生体复制和持久性,我们将执行 基因组比较,生化实验,以测试宿主产生的基因产物对 内共生体细胞,以及内共生体外膜蛋白的物理和结构表征。 通过专注于实验模型,提供宿主相关细菌的易处理的例子,这项工作 将阐明细菌共生体在宿主中定植能力的机制。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Nancy A. Moran其他文献

Correction to: The genome sequence of the grape phylloxera provides insights into the evolution, adaptation, and invasion routes of an iconic pest
  • DOI:
    10.1186/s12915-020-00864-7
  • 发表时间:
    2020-09-11
  • 期刊:
  • 影响因子:
    4.500
  • 作者:
    Claude Rispe;Fabrice Legeai;Paul D. Nabity;Rosa Fernández;Arinder K. Arora;Patrice Baa-Puyoulet;Celeste R. Banfill;Leticia Bao;Miquel Barberà;Maryem Bouallègue;Anthony Bretaudeau;Jennifer A. Brisson;Federica Calevro;Pierre Capy;Olivier Catrice;Thomas Chertemps;Carole Couture;Laurent Delière;Angela E. Douglas;Keith Dufault-Thompson;Paula Escuer;Honglin Feng;Astrid Forneck;Toni Gabaldón;Roderic Guigó;Frédérique Hilliou;Silvia Hinojosa-Alvarez;Yi-min Hsiao;Sylvie Hudaverdian;Emmanuelle Jacquin-Joly;Edward B. James;Spencer Johnston;Benjamin Joubard;Gaëlle Le Goff;Gaël Le Trionnaire;Pablo Librado;Shanlin Liu;Eric Lombaert;Hsiao-ling Lu;Martine Maïbèche;Mohamed Makni;Marina Marcet-Houben;David Martínez-Torres;Camille Meslin;Nicolas Montagné;Nancy A. Moran;Daciana Papura;Nicolas Parisot;Yvan Rahbé;Mélanie Ribeiro Lopes;Aida Ripoll-Cladellas;Stéphanie Robin;Céline Roques;Pascale Roux;Julio Rozas;Alejandro Sánchez-Gracia;Jose F. Sánchez-Herrero;Didac Santesmasses;Iris Scatoni;Rémy-Félix Serre;Ming Tang;Wenhua Tian;Paul A. Umina;Manuella van Munster;Carole Vincent-Monégat;Joshua Wemmer;Alex C. C. Wilson;Ying Zhang;Chaoyang Zhao;Jing Zhao;Serena Zhao;Xin Zhou;François Delmotte;Denis Tagu
  • 通讯作者:
    Denis Tagu
Two gut community enterotypes recurin diverse bumblebee species
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
  • 作者:
    Li Jilian;J. Elijah Powell;Guo Jun;Jay D. Evans;Wu Jie;Paul Williams;Lin Qinhui;Nancy A. Moran;Zhang Zhigang
  • 通讯作者:
    Zhang Zhigang
Weitere Übersichtsartikel aus dem Gesamtgebiet der Genetik schließen sich an
遗传疾病的整体研究
  • DOI:
  • 发表时间:
    2010
  • 期刊:
  • 影响因子:
    0
  • 作者:
    J. Lederberg;Thomas C. Rindfleisch;Leonard A. Herzenberg;Xin Zhao;James R. Broach;N. Kröger;Kent W. Hunter;N. P. Crawford;Stephen P. Goff;Nancy A. Moran;John P. McCutcheon;Eduardo P. C. Rocha;M. Surette;Transposon Tn;Fachgebiet der Genetik;S. Redlhammer
  • 通讯作者:
    S. Redlhammer
Glyphosate Effects on Growth and Biofilm Formation in Bee Gut Symbionts and Diverse Associated Bacteria
草甘膦对蜂肠共生体和多种相关细菌生长和生物膜形成的影响
  • DOI:
    10.1101/2024.03.20.585985
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Erick V. S. Motta;Tyler K. de Jong;Alejandra Gage;Joseph A. Edwards;Nancy A. Moran
  • 通讯作者:
    Nancy A. Moran
Intracellular defensive symbiont is culturable and capable of transovarial, vertical transmission
细胞内防御共生体是可培养的并且能够跨卵巢、垂直传播
  • DOI:
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Gerald P. Maeda;Mary Katherine Kelly;Aadhunik Sundar;Nancy A. Moran
  • 通讯作者:
    Nancy A. Moran

Nancy A. Moran的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Nancy A. Moran', 18)}}的其他基金

Elucidating the molecular bases of species interactions in host-associated bacterial communities
阐明宿主相关细菌群落中物种相互作用的分子基础
  • 批准号:
    10165745
  • 财政年份:
    2019
  • 资助金额:
    $ 39.13万
  • 项目类别:
Elucidating the molecular bases of species interactions in host-associated bacterial communities
阐明宿主相关细菌群落中物种相互作用的分子基础
  • 批准号:
    10620345
  • 财政年份:
    2019
  • 资助金额:
    $ 39.13万
  • 项目类别:
Host and symbiont determinants of colonization by a co-evolved gut community
共同进化的肠道群落定植的宿主和共生体决定因素
  • 批准号:
    8603715
  • 财政年份:
    2014
  • 资助金额:
    $ 39.13万
  • 项目类别:

相似海外基金

RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
  • 批准号:
    2327346
  • 财政年份:
    2024
  • 资助金额:
    $ 39.13万
  • 项目类别:
    Standard Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
  • 批准号:
    2312555
  • 财政年份:
    2024
  • 资助金额:
    $ 39.13万
  • 项目类别:
    Standard Grant
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
  • 批准号:
    BB/Z514391/1
  • 财政年份:
    2024
  • 资助金额:
    $ 39.13万
  • 项目类别:
    Training Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
  • 批准号:
    ES/Z502595/1
  • 财政年份:
    2024
  • 资助金额:
    $ 39.13万
  • 项目类别:
    Fellowship
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
  • 批准号:
    ES/Z000149/1
  • 财政年份:
    2024
  • 资助金额:
    $ 39.13万
  • 项目类别:
    Research Grant
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
  • 批准号:
    23K24936
  • 财政年份:
    2024
  • 资助金额:
    $ 39.13万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
  • 批准号:
    2901648
  • 财政年份:
    2024
  • 资助金额:
    $ 39.13万
  • 项目类别:
    Studentship
ERI: Developing a Trust-supporting Design Framework with Affect for Human-AI Collaboration
ERI:开发一个支持信任的设计框架,影响人类与人工智能的协作
  • 批准号:
    2301846
  • 财政年份:
    2023
  • 资助金额:
    $ 39.13万
  • 项目类别:
    Standard Grant
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
  • 批准号:
    488039
  • 财政年份:
    2023
  • 资助金额:
    $ 39.13万
  • 项目类别:
    Operating Grants
How motor impairments due to neurodegenerative diseases affect masticatory movements
神经退行性疾病引起的运动障碍如何影响咀嚼运动
  • 批准号:
    23K16076
  • 财政年份:
    2023
  • 资助金额:
    $ 39.13万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了