Line-scanned MEMS-based dual-axis confocal microscopy to detect oral lesions in v

基于线扫描 MEMS 的双轴共焦显微镜可检测口腔病变

• 批准号: 8725638
• 负责人: Jonathan T.C. Liu
• 金额: $ 83.91万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8725638
• 关键词: Architecture; Benign; Biopsy; Caliber; Cell Nucleus; Cessation of life; Clinic; Clinical; Clinical Research; Confocal Microscopy; Dermatology; Detection; Devices; Diagnosis; Diagnostic; Diagnostic Specificity; Disease; Engineering; Epithelium; Equilibrium; Excision biopsy; Feasibility Studies; Goals; Gold; Hand; Head and neck structure; Health; Histology; Histopathology; Human; Image; Imagery; Imaging Techniques; Imaging technology; Lesion; Malignant - descriptor; Malignant Neoplasms; Memorial Sloan-Kettering Cancer Center; Methods; Microscope; Microscopy; Miniaturization; Morphologic artifacts; Morphology; Motion; Optics; Oral cavity; Oral mucous membrane structure; Pathology; Patient Noncompliance; Patients; Performance; Premalignant; Preparation; Protocols documentation; Resolution; Sampling; Scanning; Screening for Oral Cancer; Sensitivity and Specificity; Site; Specificity; Specimen; Speed; System; Thick; Time; Tissue Sample; Tissues; Training; Triage; United States; base; cellular imaging; cohort; cost; economic implication; improved; in vivo; malignant mouth neoplasm; non-compliance; oral lesion; prototype; public health relevance; research study; simulation; technology development; tissue phantom; usability

DESCRIPTION (provided by applicant): Oral cancer is the fifth most common malignancy worldwide, with roughly 50,000 new cases and over 10,00 deaths from this disease each year in the United States. Otorhinolaryngologists are trained to recognize aberrations in gross morphology that are indicative of malignancy, as well as to utilize a number of wide-field imaging techniques to improve the visualization of lesions, some of which can detect disease with high sensitivity but poor specificity. As a result of poor diagnostic specificity, the majorit of suspected lesions, when biopsied and analyzed via histopathology, reveal benign conditions rather than premalignant or malignant lesions. In addition to the high costs and time associated with obtaining large numbers of unnecessary tissue samples for pathological analysis, the need for an invasive biopsy often results in patient discomfort, noncompliance, complications and/or diagnostic delays. Reflectance confocal microscopy can potentially provide a real-time non-invasive method to triage and guide excisional biopsy. However, miniaturization is a challenge and previous in vivo systems have necesitated trade-offs between critical parameters such as device size, imaging speed (frame rate), imaging depth, resolution, and field-of-view. The goal of this project is to develop a miniature line-scanned reflectance microscope that combines, for the first time, a dual-axis confocal architecture with MEMS-based beam scanning to achieve an optimized clinical device for real-time micropathological detection of oral lesions. This hand-held device, with a tip diameter of 2 mm, wil enable high-contrast sub-cellular imaging deep within tissues of the oral cavity. Furthermore, high- speed (>30 Hz) optical sectioning wil be achieved, which will greatly enhance the clinical usability of this device by minimizing motion artifacts and enabling high-quality real-time image mosaicing. Monte-Carlo scattering simulations, and experiments with phantoms and tissue specimens, will be performed to rigorously quantify and optimize the performance of line-scanned dual-axis confocal microscopy. Finally, in the third and final year of this technology-development project, a clinical feasibility study will be performed to assess the sensitivity and specificity of our diagnostic prototype and to justify further translational efforts.

描述（由申请人提供）：口腔癌是全球第五大最常见的恶性肿瘤，在美国，每年大约有50,000例新病例，该病死亡10,00多人。耳鼻喉科医生经过训练，可以识别出表明恶性肿瘤的总形态的畸变，并利用多种宽视野成像技术来改善病变的可视化，其中一些可以检测出具有较高敏感性但特异性较差的疾病。由于诊断特异性差，通过组织病理学进行活检和分析时，多数可疑的病变揭示了良性疾病，而不是预签名或恶性病变。除了获得大量不必要的组织样本进行病理分析相关的高成本和时间外，对侵入性活检的需求通常会导致患者不适，不合规，并发症和/或诊断延迟。反射率共聚焦显微镜可以潜在地提供一种实时的非侵入性方法来进行分类和引导移动活检。但是，小型化是一个挑战，先前的体内系统在关键参数（例如设备尺寸，成像速度（帧速率），成像深度，分辨率和视野的关键参数之间都具有必要的权衡。该项目的目的是开发一个微型线条扫描的反射显微镜，该显微镜首次结合了双轴共聚焦体系结构，并具有基于MEMS的光束扫描，以实现优化的临床设备，以实现口腔病变的实时微病理检测。这个手持 设备的尖端直径为2毫米，可以使高对比度的亚细胞成像在口腔内部的组织深处。此外，将实现高速（> 30 Hz）的光学切片，这将通过最大程度地减少运动伪像和 实现高质量的实时图像镶嵌。将进行严格量化和优化线扫描的双轴共聚焦显微镜的性能，并对幻象和组织标本进行蒙特卡洛散射模拟以及实验。最后，在该技术开发项目的第三年也是最后一年中，将进行一项临床可行性研究，以评估我们诊断原型的敏感性和特异性并证明进一步的翻译工作合理。