Nanoparticle-based SERS assay for Dengue virus detection in blood

描述：登革病毒(DENV)是一种由蚊子传播的RNA病毒，可导致人类出血热、休克，严重者甚至死亡。DENV也被证明是一种通过血液传播的病毒。AABB的输血传播疾病委员会最近确定DENV是一种高度优先的新兴传染病病原体，在美国具有潜在的输血传播风险。目前，没有针对DENV进行血液筛查的措施。大多数临床和研究实验室检测DENV的方法是核酸扩增法和酶联免疫吸附试验(ELISA)。核酸扩增的成本很高，而且ELISA很耗时。因此，开发一种廉价、快速、灵敏的DENV检测方法对于降低输血感染风险和提高及时监测至关重要。在这一需求的推动下，在本项目中，我们将开发一种基于纳米金的、快速、灵敏和成本效益高的无标记表面增强拉曼光谱(SERS)方法，用于选择性检测血液中的DENV。我们预计，这种SERS方法将把试剂成本降低到核酸扩增分析成本的十分之一，检测下限可以低于10个病毒/毫升。此外，SERS方法还可以发展成为一种适合于血液筛查设施的自动化筛选方法。

DESCRIPTION: Dengue virus (DENV), a mosquito-transmitted RNA virus, can cause human hemorrhagic fever, shock and even death, in severe cases. DENV has also been documented as a blood transfusion-transmitted virus. AABB's Transfusion-Transmitted Diseases Committee has recently identified DENV as a high-priority emerging infectious agent with potential risk of transfusion transmission in the United States. Currently, there is no blood screening measure conducted for DENV. Detection methods for DENV in most clinical and research laboratories are nucleic acid amplification assays and enzyme-linked immunosorbent assays (ELISAs). The cost for nucleic acid amplification is high and ELISAs are time-consuming. Therefore, developing an inexpensive, fast, and sensitive test for detection of DENV is crucial to reduce the risk of transfusion-mediated infection and improve timely surveillance. Driven by this need, in this project, we will develop a gold nanoparticle-based, rapid, sensitive and cost-effective label-free Surface Enhanced Raman Spectroscopy (SERS) assay for selective DENV detection in blood. We expect this SERS assay will reduce the reagent cost to 1/10th the cost of nucleic acid amplification assay and detection limit can be below 10 viruses /ml. Furthermore, the SERS assay can be developed into an automated screening assay, which is suitable in blood screen facilities.