Time-keeping Mechanisms in Drosophila Embryonic Development

果蝇胚胎发育的计时机制

• 批准号: 8839511
• 负责人: Stefano Di Talia
• 金额: $ 24.9万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-05 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8839511
• 关键词: Address; Affect; Award; Biochemical; Biological; Biological Sciences; Cell Cycle; Cell Cycle Regulation; Cell Fate Control; Cell division; Cells; Cellular biology; Congenital Abnormality; Controlled Study; DNA biosynthesis; Development; Developmental Biology; Doctor of Philosophy; Drosophila genus; Educational process of instructing; Embryo; Embryology; Embryonic Development; Enhancers; Ensure; Feedback; Fellowship; Gene Expression; Genetic; Genetic Screening; Genetic Techniques; Genetic Transcription; Image; Laboratories; Life; Marines; Measures; Mitosis; Molecular; Molecular Biology; Molecular Genetics; Noise; Nuclear; Organism; Pathway interactions; Phase; Regulation; Regulator Genes; Reproducibility; Research; Role; Schedule; Signal Transduction; Signaling Protein; System; Techniques; Testing; Time; Training; United States National Institutes of Health; Universities; abstracting; career; cell behavior; gastrulation; genetic regulatory protein; insight; mathematical model; mutant; novel; photo switch; professor; programs; protein degradation; research study; response; skills

Abstract The candidate is currently a Life Science Research Fellow in the laboratory of Professor Eric Wieschaus in the Department of Molecular Biology at Princeton University. The candidate was awarded a PhD from The Rockefeller University for research in quantitative cell biology. During the Postdoctoral Fellowship, the candidate is transitioning to the field of developmental biology. The candidate will apply the quantitative and analytical techniques from previous research as well as further training in genetics and developmental biology to the study of the control of timing of cell behaviors during embryonic development. The NIH Pathway to Independence Award would provide necessary support to the candidate during this transition period. The award would allow the candidate to acquire new skills in genetics and developmental biology as well as to establish novel research directions. The candidate will benefit from the opportunity to take the graduate course 'Genetics of Multicellular Organisms' at Princeton University as well as the courses 'Eukaryotic Gene Expression' and 'Gene Regulatory Networks for Development' at Cold Spring Harbor Laboratory and at the Marine Biological Laboratory respectively. The candidate will study the molecular mechanisms ensuring precise temporal regulation of cell division through control of gene expression, signaling and protein degradation during Drosophila embryonic development. During the K99 phase of the award, the candidate will 1) Develop theoretical analysis of the integration time of signaling systems 2) Perform genetic screens and molecular biology experiments to identify regulators of Cdc25 transcription (rate-limiting activator of the cell cycle) 3) Determine the importance of regulation of Cdc25 protein degradation at the maternal-to-zygotic transition, a critical developmental transition. These aims will be accomplished by combining genetics, embryology, molecular biology, quantitative live imaging and mathematical modeling. During the R00 phase of the award, the candidate will extend the research by determining the molecular mechanisms ensuring that Cdc25 is transcribed and degraded with high temporal precision and by analyzing signaling systems beyond cell cycle control. The candidate ultimately desires to pursue an academic career in research and teaching.

抽象的 该候选人现为Eric教授实验室生命科学研究研究员 维绍斯在普林斯顿大学分子生物学系工作。该候选人被授予 洛克菲勒大学博士学位，从事定量细胞生物学研究。博士后期间 奖学金候选人正在转向发育生物学领域。候选人将申请 先前研究的定量和分析技术以及遗传学的进一步培训 和发育生物学研究胚胎期间细胞行为的时间控制 发展。 NIH 独立之路奖将为 过渡时期的候选人。该奖项将使候选人能够获得以下方面的新技能 遗传学和发育生物学以及建立新的研究方向。候选人 将受益于参加研究生课程“多细胞生物遗传学”的机会 普林斯顿大学以及“真核基因表达”和“基因调控”课程 冷泉港实验室和海洋生物实验室的发展网络 分别。候选人将研究确保精确时间调节的分子机制 通过控制果蝇的基因表达、信号传导和蛋白质降解来控制细胞分裂 胚胎发育。在奖励的 K99 阶段，候选人将 1) 发展理论 分析信号系统的整合时间 2) 进行遗传筛选和分子生物学 鉴定 Cdc25 转录调节因子（细胞周期限速激活因子）的实验 3) 确定母体到合子过程中 Cdc25 蛋白降解调节的重要性 转变，关键的发展转变。这些目标将通过结合遗传学来实现， 胚胎学、分子生物学、定量实时成像和数学建模。 R00期间 在奖励阶段，候选人将通过确定分子机制来扩展研究 确保 Cdc25 以高时间精度转录和降解，并通过分析 超出细胞周期控制的信号系统。候选人最终希望追求学术 从事研究和教学工作。