Development of a lab-on-a-chip for point-of-care biomonitoring of blood metals

开发用于血液金属即时生物监测的芯片实验室

• 批准号: 8730155
• 负责人: Ian Papautsky
• 金额: $ 32.91万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-04 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8730155
• 关键词: Academic achievement; Address; Age; Biological; Biological Markers; Biological Monitoring; Biological Sciences; Blood; Blood - brain barrier anatomy; Blood Vessels; Blood specimen; Brain; Cadmium; Caring; Chemistry; Child; Childhood; Clinic; Clinical; Clinical Research; Collaborations; Collection; Communities; Controlled Study; Data Analyses; Detection; Development; Devices; Drops; Electrical Engineering; Elements; Engineering; Enrollment; Ensure; Environment; Environmental Health; Epidemiology; Equipment; Exposure to; Future; Goals; Health; Healthcare; Heavy Metals; Human Resources; Hypersensitivity; Individual; Industry; International; Kidney; Laboratories; Laboratory Procedures; Lead; Liver; Manganese; Marketing; Measurement; Measures; Metal exposure; Metals; Methods; Microfluidics; Monitor; Nature; Occupational; Ohio; Outcome; Parents; Performance; Peripheral arterial disease; Point-of-Care Systems; Population; Population Research; Positioning Attribute; Process; Public Health; Recording of previous events; Recruitment Activity; Research; Research Personnel; Resources; Risk; Rural; Rural Population; Sampling; School-Age Population; Site; Technology; Testing; Time; Toxic effect; Urban Population; Venipunctures; Whole Blood; Work; adverse outcome; base; blood lead; bone; cohort; commercialization; cost; diabetic; drinking water; glucose monitor; improved; industry partner; lead concentration; lead exposure; male health; micro-total analysis system; multidisciplinary; neurotoxic; novel; point of care; public health relevance; sample collection; sensor; stem

DESCRIPTION (provided by applicant): This application seeks to transform the current paradigm for blood metal analysis through the development of a sensor that will provide rapid and accurate point-of-care measurement of blood metals in susceptible pediatric populations. Biomarkers of pediatric, low-level, mixed exposures to lead (Pb), manganese (Mn), and cadmium (Cd) are essential. There is a clear need for improved technology to quantitatively assess and identify adverse consequences of these exposures. Current approaches suffer from high costs including extensive labor, equipment and time-consuming laboratory procedures, and often demonstrate long turnaround times. This project addresses this need by developing a novel sensor technology for simultaneous and swift measurement of heavy metals in whole blood. Our approach is to integrate anodic stripping voltammetry with our deep expertise in lab-on-a-chip and microfluidics. Our multidisciplinary team of engineers, chemists, and clinical/environmental health experts has already taken the first steps towards developing such a device and has demonstrated feasibility of the sensor through pilot tests. We are now poised to fully develop the sensor system for point-of-care application. This project will be the first to develop an environmentally-friendly lab-on- a-chip sensor for point-of-care multi-metal analysis, which will provide real-time analysis of metals for susceptible populations, in national and international research and clinical settings. Collaboration with our commercialization partner will position the multi-metal sensor for wide-spread use in clinical and research settings.

描述（由申请人提供）：本申请旨在通过开发一种传感器来改变血液金属分析的当前模式，该传感器将在易感儿科人群中提供快速准确的血液金属即时测量。儿童低水平混合接触铅（Pb）、锰（Mn）和镉（Cd）的生物标志物至关重要。显然需要改进技术，以便从数量上评估和查明这些照射的不利后果。目前的方法具有高成本的缺点，包括大量的劳动力、设备和耗时的实验室程序，并且通常表现出长的周转时间。该项目通过开发一种新的传感器技术来同时快速测量全血中的重金属来满足这一需求。我们的方法是将阳极溶出伏安法与我们在芯片实验室和微流体方面的深厚专业知识相结合。我们的工程师、化学家和临床/环境健康专家组成的多学科团队已经迈出了开发这种设备的第一步，并通过试点测试证明了传感器的可行性。我们现在准备全面开发用于护理点应用的传感器系统。该项目将是第一个 开发一种用于即时多金属分析的环境友好型芯片实验室传感器，这将在国家和国际研究和临床环境中为易感人群提供实时金属分析。与我们的商业化合作伙伴的合作将 定位多金属传感器，以便在临床和研究环境中广泛使用。