Multi-Metal Chip for Point-of-Care Monitoring in Children

描述（由申请人提供）：本申请寻求开发一种“多金属芯片”，其将促进儿童血液金属浓度的快速和准确的即时测量，所述血液金属浓度包括锰（Mn）、锌（Zn）、镉（Cd）和铅（Pb）。全血分析是确定金属暴露和评估健康结果的最常用方法，但从儿童身上采集样本面临多重挑战，包括父母同意和儿童同意，以及献血的能力（特别是在需要重复测量的研究中）。该应用旨在通过展示一种能够快速分析（5- 10分钟）少量血液样本（来自单次刺血针穿刺的2滴）的“芯片实验室”传感器来改变当前的范例。我们建议通过实现以下具体目标来开发用于全血中Mn、Zn、Cd和Pb的高灵敏度测量的系统：1）开发用于全血中快速、即时多金属分析的芯片实验室传感器，以及2）进行现场测试并确定用于测量居住在锰铁精炼厂附近的儿童的血液金属浓度的多金属传感器的适用性。我们的方法是将阳极溶出伏安法与我们在芯片实验室和微流体方面的深厚专业知识相结合。我们的工程师、化学家和环境健康多学科团队已经朝着开发这种设备迈出了第一步。我们已经通过试点测试证明了传感器的可行性，现在准备全面开发用于护理点应用的传感器系统。本研究的独特之处在于仅需几滴血液进行分析的儿童友好要求、低成本（一次性）、进行多次血液测量的能力以及其在大规模临床、职业和研究环境中的潜在用途，例如国家儿童研究和国家健康和营养检查调查。 公共卫生相关性：本申请旨在开发一种“芯片实验室”传感器，用于快速准确地测量金属（锰、锌、镉和铅）血液。全血分析是确定金属暴露和评估健康结果的最常用方法，但从儿童身上采集样本存在很大问题。本研究的独特之处在于仅需几滴血液进行分析的儿童友好型要求、低成本、进行多次血液测量的能力以及在大规模临床、职业和研究环境中的潜在用途。

DESCRIPTION (provided by applicant): This application seeks to develop a "multi-metal chip" that will facilitate rapid and accurate point- of-care measurement of blood metal concentration, including manganese (Mn), zinc (Zn), cadmium (Cd), and lead (Pb) in children. Whole blood analysis is the most common method for determining metal exposure and assessment of health outcomes, yet sample collection from children poses multiple challenges, including parent consent and child assent, and the ability to give blood (especially in studies that require repeated measurements). This application aims to transform the current paradigm by demonstrating a "laboratory-in-a-chip" sensor capable of rapid analysis (5-10min) on small blood samples (2 drops from a single lancet puncture). We propose to develop this system for highly sensitive measurement of Mn, Zn, Cd, and Pb in whole blood by accomplishing the following specific aims: 1) develop a lab-on-a-chip sensor for a rapid, point-of-care multi-metal analysis in whole blood, and 2) conduct a field test and determine the applicability of the multi-metal sensor for measuring blood metal concentration in children residing near a ferromanganese refinery. Our approach will be to integrate anodic stripping voltammetry with our deep expertise in lab-on-a-chip and microfluidics. Our multidisciplinary team of engineers, chemists, and environmental health has taken the first steps towards developing such a device. We have demonstrated feasibility of the sensor through pilot tests, and are now poised to fully develop the sensor system for point-of-care application. The uniqueness of this study is the child-friendly requirement of only drops of blood for analysis, low- cost (disposable), ability to conduct multiple blood measurements, and its potential use in large- scale clinic, occupational and research settings, such as the National Children's Study and the National Health and Nutrition Examination Survey. PUBLIC HEALTH RELEVANCE: This application seeks to develop a "laboratory-in-a-chip" sensor for rapid and accurate point-of- care measurement of metals (manganese, zinc, cadmium, and lead) blood. Whole blood analysis is the most common method for determining metal exposure and assessment of health outcomes, yet sample collection from children is highly problematic. The uniqueness of this study is the child-friendly requirement of only drops of blood for analysis, low-cost, ability to conduct multiple blood measurements, and potential use in large-scale clinic, occupational and research settings.