Investigating Biogeochemical Controls on Metal Mixture Toxicity Using Stable Isot

使用稳定 isot 研究金属混合物毒性的生物地球化学控制

• 批准号: 8758202
• 负责人: James Ranville
• 金额: $ 20.82万
• 依托单位: COLORADO SCHOOL OF MINES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-18 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8758202
• 关键词: Accounting; Address; Affect; Bioavailable; Biological; Biological Assay; Biological Availability; Chemicals; Colorado; Communities; Complex; Data; Detection; Development; Diagnostic; Ecosystem; Effectiveness; Effectiveness of Interventions; Environment; Evaluation; Failure; Food Chain; Funding; Genomics; Goals; Grant; Health; Human; Individual; Industry; Intervention; Knowledge; Lead; Link; Measurement; Metal exposure; Metals; Methodology; Methods; Mining; Modeling; Molecular; Monitor; National Institute of Environmental Health Sciences; Natural experiment; Organism; Phase; Practice Management; Process; Recovery; Regulation; Request for Proposals; Research; Research Infrastructure; Research Methodology; Risk; Risk Assessment; Signal Recognition Particle; Site; Solid; Source; Staging; Stream; Superfund; System; Techniques; Testing; Tissues; Toxic effect; Translational Research; Water; expectation; fundamental research; hazard; improved; interest; metal poisoning; programs; public health relevance; real world application; remediation; response; stable isotope; superfund site; tool; uptake; wasting; water quality; water treatment

DESCRIPTION (provided by applicant): Assessing site-specific bioavailability is an important consideration in determining and affectively addressing hazards posed by metals-contaminated waste sites. This application addresses the NIEHS Superfund Research Program's needs for fundamental research that improves the understanding of the effects of biogeochemical interactions on contaminant bioavailability. Because metals occur in mixtures rather than as single contaminants at many contaminated sites, accurate assessment of metal exposure and effects to humans and ecosystems must account for mixture effects, which could be additive, less than additive, or more than additive. However, current water quality regulations and management practices usually address individual metals, because adequate tools are not yet available for predicting bioavailability and toxicity of metal mixtures. Our long-term objective isto detect, characterize, and assess the risks posed by contaminant metal mixtures. To accomplish that goal, we need to develop and/or refine advanced techniques for the detection, assessment, and evaluation of metal bioavailability. Those techniques will include environmental molecular diagnostics and stable isotope assays. In the proposed research, the methods we develop under controlled conditions will be "field-truthed" in a metals-contaminated stream at the North Fork Clear Creek Superfund site in central Colorado. The approaches and tools developed for that site will provide a physical and intellectual decision infrastructure applicable to other meta-contaminated sites. In this project a combination of organism- and community-level response studies (Project 1); genomic bioassays of organism response to multiple-metals exposure (Project 2); and measurements of bioavailable water-column metals and tissue-metal residues (Project 3) will be developed and applied. We will gain an understanding of the flux between biological/chemical/geological interfaces as it relates to bioavailability and remediation effectiveness. Furthermore, we will elucidate the geochemical factors affecting biological uptake (e.g., transport into the food chain). We will also conduct a "natural experiment" in the metal-contaminated stream in which we will examine responses before, during, and after installation of a water-treatment system that will decrease concentrations of the metals. Using the methods developed and tested during the first two years of the project, we will be able to model and observe the effectiveness of the remediation efforts during the later years of the project. Finally we have delineated a plan for "research translation", including engaging end-users (e.g., regulatory agencies, metals-industry representatives, and toxicity modelers) throughout the duration of the grant. Although the focus of the proposed research is a single Superfund site, the methodology and results will be applicable to metal mixtures in many receiving waters, regardless of the contamination source.

描述（由申请者提供）：评估特定场地的生物利用度是确定和有效解决金属污染废物场地所造成危害的一个重要考虑因素。该申请解决了NIEHS超级基金研究计划的基础研究的需求，提高了对污染物生物利用度的生物化学相互作用的影响的理解。由于在许多受污染场地，金属以混合物形式出现，而不是作为单一污染物出现，因此，对金属接触及其对人类和生态系统的影响的准确评估必须考虑到混合物效应，这种效应可能是相加的、小于相加的或大于相加的。然而，目前的水质法规和管理做法通常针对个别金属，因为还没有足够的工具来预测金属混合物的生物利用度和毒性。我们的长期目标是检测、表征和评估污染金属混合物带来的风险。为了实现这一目标，我们需要开发和/或完善先进的技术来检测，评估和评价金属的生物利用度。这些技术将包括环境分子诊断和稳定同位素分析。在拟议的研究中，我们在受控条件下开发的方法将在科罗拉多中部的北福克清溪超级基金场地的金属污染流中进行“实地验证”。为该场址开发的方法和工具将提供适用于其他受亚污染场址的物质和知识决策基础设施。 在该项目中，将开发和应用有机体和社区一级的反应研究（项目1）;有机体对多种金属接触的反应的基因组生物测定（项目2）;以及生物可利用的水柱金属和组织金属残留物的测量（项目3）。我们将了解生物/化学/地质界面之间的通量，因为它涉及生物利用度和修复效果。此外，我们将阐明影响生物吸收的地球化学因素（例如，进入食物链）。我们还将在受金属污染的河流中进行“自然实验”，在该实验中，我们将检查安装水处理系统之前、期间和之后的反应，该系统将降低金属的浓度。使用在项目的前两年开发和测试的方法，我们将能够在项目的后几年建模和观察补救工作的有效性。最后，我们制定了一个“研究翻译”计划，包括吸引最终用户（例如，监管机构、金属行业代表和毒性建模人员）。虽然拟议研究的重点是一个单一的超级基金网站，方法和结果将适用于金属混合物在许多接收沃茨，无论污染源。