West Coast Central Comprehensive Metabolomics Resource Core (WC3MRC)

描述（由申请人提供）：西海岸中央综合代谢组学资源核心（WC 3 MRC）将通过代谢组学中心服务核心提供综合服务，并通过代谢组学高级服务核心提供高级服务，包括方法开发。结合起来，WC 3 MRC控制着30多个质谱仪，5个核磁共振仪器和尖端成像设备，以及内部的计算机集群，以及用于代谢组学数据采集，数据处理和数据分析（包括路径映射）的开放访问和商业软件。统计支持，工具开发和高级代谢网络分析将在基因组学集成核心中进行，而试点和可行性项目以及课程和研讨会将由推广和推广核心组织，由加州大学戴维斯分校临床和转化科学中心（CISC）领导。数据中心的整体管理和数据传输将由管理核心执行。 具体来说，WC 3 MRC将成为美国第一个提供超过1,000种已鉴定代谢物的定量靶向的中心。 各种各样的生化途径。对于所有这些代谢物，均提供了参比标准品，并对样品制备、质谱和色谱条件进行了验证。此外，WC 3 MRC将通过精确的质谱法使用非靶向代谢组学方法，用于发现驱动的项目，包括化合物鉴定。先进核心实验室使用的方法将自动化并转移到中央服务核心，供充值服务使用。将开发新的服务和工具，从同位素标记的通量分析到图像引导的代谢组学，这些服务和工具将与使用成像设施的既定客户联系起来。将通过对HumanCyc酶的管理和使用InChI结构标识符来改进途径注释，InChI结构标识符将用于构建完整的代谢网络，随后用于途径富集统计分析。 当地和区域科学家将通过年度竞争性试点和可行性奖参与。 参与和奖励标准已经制定，并将由CTSC进行。培训计划将在技术和医学层面上教育下一代代谢组学科学家。

DESCRIPTION (provided by applicant): The West Coast Central Comprehensive Metabolomics Resource Core (WC3MRC) will offer integrated services through a Metabolomics Central Service Core, and offer advanced services, including method developments, through the Metabolomics Advanced Services Core. Combined, the WC3MRC commands over 30 mass spectrometers, 5 NMR instruments and cutting-edge imaging equipment, and computer clusters with in-house as well as open-access and commercial software for metabolomic data acquisition, data processing, and data analysis including pathway mapping. Statistical support, tool development and advanced metabolic network analysis will be conducted in the Genomics Integration Core, while pilot and feasibility projects as well as courses and workshops will be organized by the Promotion and Outreach Core, to be led by the UC Davis Clinical and Translational Science Center (CISC). Overall management and data transfer to the Data Center will be performed by the Administrative Core. Specifically, the WC3MRC will be the first center in the United States that offers quantitative targeting of over 1,000 identified metabolites over a wide variety of biochemical pathways. For all these metabolites, reference standards are available and sample preparation, mass spectra and chromatographic conditions have been validated. Additionally, the WC3MRC will use untargeted metabolomic methods by accurate mass spectrometry for discovery-driven projects, including compound identifications. Methods used in the Advanced Core laboratories will be robotized and transferred to the Central Service Core for use by recharge fee services. Novel services and tools will be developed, ranging from isotope-labeled flux analysis to image guided metabolomics that will link to the established clientele using the imaging facility. Pathway annotations will be improved through curation of HumanCyc enzymes and by using InChI structure identifiers that will be used to construct complete metabolic networks that are subsequently used for pathway over enrichment statistical analysis. Both local and regional scientists will be engaged through annual, competitive pilot and feasibility awards. Participation and award criteria have been worked out and will be conducted by the CTSC. Training programs will educate the next generation of metabolomics scientists, both on the technical and the medical level.