Bruker timsTOF pro LC-MS system

项目摘要_摘要 我们正在申请一台Bruker TimsTOF Pro囚禁离子迁移率质谱仪LC- MS系统。这种LC-ion Mobility MS系统提供了优于其他系统的分辨率 提供更深入的代谢组学、类脂组学和暴露组筛选能力 经典LC-精确质谱质谱联用仪。随着所请求的 Bruker TimsTOF Pro LC-MS系统，结构相似的脂类和其他化合物都可以 准确检测和识别。迫切需要这样的系统来填补未得到满足的缺口需求 加州大学戴维斯分校的生物医学社区。我们已经确定了该仪器的8个主要用户 80%的时间使用率，并建议加州大学戴维斯分校代谢组学核心将20%的时间使用率 为小用户开展基于充值的项目。 通过提供我们在LC-MS代谢组学方面的基础设施和技术专长，我们计划 与我们的用户进行多样化和长期的合作。所有项目的共同主线是 化学络合物太大了，不能用目前的LC-精确质量‘在深处’分解 仅MS/MS。用户需要进入下一阶段来充分解决他们的研究问题。 需要离子移动性的项目有：环境健康(Young：深部MS/MS 非靶向筛查报道，van Winkle：氧化脂质的更高分辨率)，癌症 新陈代谢(Carraway：提高BMP和PG脂质组学的敏感性和分辨率 细胞器，Chen：改进的氧固醇异构体的拆分)，昼夜循环(Chiu：拆分 等压和同分异构三酸甘油酯)、营养干预(斯坦霍普：解决尿液 多酚)，化合物鉴定方法(Fiehn：提高结构的可信度 CCS值注释)与肠道微生物群的变化(Lasalle：肠道-脑轴的相互作用 雷特综合征，粪便中生物胺的分解)。所有这些项目都有一个共同点 他们在我们正在进行的合作中遇到了障碍，因为我们的能力 WCMC目前的仪器缺乏额外的离子迁移率分离能力，尽管 我们在准确的质量分析方面的精湛能力。

Project Summary_Abstract We are applying for funds for a Bruker timsTOF Pro trapped ion mobility mass spectrometer LC- MS system. This LC-ion mobility MS systems offers superior resolution to alternative systems providing much deeper metabolomics, lipidomics and exposome screening capabilities than classic LC-accurate mass MS/MS instruments. With the enhanced performance of the requested Bruker timsTOF Pro LC-MS system, structurally similar lipids and other compounds can be accurately detected and identified. Such system is urgently needed to fill gaping unmet needs by the biomedical community here at UC Davis. We have identified 8 major users for this instrument for 80% of time usage and suggest a 20% time usage by the UC Davis metabolomics core to work on recharge-based projects for minor users. By providing our infrastructure and technical expertise on LC-MS metabolomics, we project a diverse and long-term collaborations with our users. The common thread across all projects is that chemical complexities are too large to be resolved ‘in depths’ by current LC-accurate mass MS/MS alone. Users need to get to the next stage to solve their research problems adequately. Projects that need for ion mobility are such as: environmental health (Young: deeper MS/MS coverage for non-targeted screening, van Winkle: higher resolution for oxidized lipids), cancer metabolism (Carraway: improved sensitivity and resolution for BMP and PG lipidomics in organelles, Chen: improved resolution of oxysterol isomers), diurnal cycles (Chiu: resolving isobaric and isomeric triacylglycerides), nutritional interventions (Stanhope: resolving urinary polyphenols), methods in compound identification (Fiehn: improving confidence in structure annotations by CCS values) to gut microbiome changes (LaSalle: interaction of gut-brain axis in Rett syndrome, resolution of biogenic amines in fecal matter). All these projects have in common that they have hit roadblocks in our ongoing collaborations with respect to capabilities of our current instrumentation at the WCMC that lack the added power of ion mobility separation despite our exquisite capabilities in accurate mass analyses.