West Coast Central Comprehensive Metabolomics Resource Core (WC3MRC)

西海岸中心综合代谢组学资源核心 (WC3MRC)

• 批准号: 9452786
• 负责人: Oliver Fiehn
• 金额: $ 47.7万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-04 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9452786
• 关键词: Award; Biochemical Pathway; Bioinformatics; Biology; Cardiovascular Diseases; Center for Translational Science Activities; Client; Clinical; Clinical Sciences; Collaborations; Computer software; Data; Data Analyses; Diabetes Mellitus; Disease; Educational workshop; Ensure; Enzymes; Equipment; Feasibility Studies; Fee-for-Service Plans; Genetic; Genomics; Health Services Research; Human; Image; Individual; Informatics; Isoenzymes; Isotope Labeling; Laboratories; Link; Malignant Neoplasms; Management Information Systems; Mass Spectrum Analysis; Medical; Metabolic; Metabolism; Methods; Pathway Analysis; Pathway interactions; Pilot Projects; Preparation; Process; Publishing; Reference Standards; Research; Research Personnel; Retreatment; Robot; Sampling; Sampling Studies; Science; Scientist; Services; Statistical Data Interpretation; Structure; Technology; Tissues; Training; Training Programs; Translational Research; United States; Visualization software; Work; computer cluster; computerized data processing; data acquisition; fighting; genomic data; image guided; improved; instrument; mass spectrometer; metabolomics; metabolomics resource; metagenomic sequencing; method development; next generation; novel; outreach; professional atmosphere; programs; statistics; support tools; tool; tool development; web site

DESCRIPTION (provided by applicant): The West Coast Central Comprehensive Metabolomics Resource Core (WC3MRC) will offer integrated services through a Metabolomics Central Service Core, and offer advanced services, including method developments, through the Metabolomics Advanced Services Core. Combined, the WC3MRC commands over 30 mass spectrometers, 5 NMR instruments and cutting-edge imaging equipment, and computer clusters with in-house as well as open-access and commercial software for metabolomic data acquisition, data processing, and data analysis including pathway mapping. Statistical support, tool development and advanced metabolic network analysis will be conducted in the Genomics Integration Core, while pilot and feasibility projects as well as courses and workshops will be organized by the Promotion and Outreach Core, to be led by the UC Davis Clinical and Translational Science Center (CISC). Overall management and data transfer to the Data Center will be performed by the Administrative Core. Specifically, the WC3MRC will be the first center in the United States that offers quantitative targeting of over 1,000 identified metabolites over a wide variety of biochemical pathways. For all these metabolites, reference standards are available and sample preparation, mass spectra and chromatographic conditions have been validated. Additionally, the WC3MRC will use untargeted metabolomic methods by accurate mass spectrometry for discovery-driven projects, including compound identifications. Methods used in the Advanced Core laboratories will be robotized and transferred to the Central Service Core for use by recharge fee services. Novel services and tools will be developed, ranging from isotope-labeled flux analysis to image guided metabolomics that will link to the established clientele using the imaging facility. Pathway annotations will be improved through curation of HumanCyc enzymes and by using InChI structure identifiers that will be used to construct complete metabolic networks that are subsequently used for pathway over enrichment statistical analysis. Both local and regional scientists will be engaged through annual, competitive pilot and feasibility awards. Participation and award criteria have been worked out and will be conducted by the CTSC. Training programs will educate the next generation of metabolomics scientists, both on the technical and the medical level.

描述(由申请人提供)：西海岸中央综合代谢组学资源核心(WC3MRC)将通过代谢组学中央服务核心提供综合服务，并通过代谢组学高级服务核心提供包括方法开发在内的高级服务。加在一起，WC3MRC拥有30多台质谱仪、5台核磁共振仪器和尖端成像设备，以及带有内部开放访问和商业软件的计算机集群，用于代谢数据采集、数据处理和数据分析(包括路径图)。统计支持、工具开发和高级代谢网络分析将在基因组学整合核心进行，而试点和可行性项目以及课程和讲习班将由加州大学戴维斯临床和转化科学中心(CISC)领导的促进和外联核心组织。总体管理和向数据中心的数据传输将由管理核心执行。具体地说，WC3MRC将是美国第一个在一年内为1000多种已识别代谢物提供定量靶向的中心 各种各样的生化途径。对于所有这些代谢物，都有参考标准，样品制备、质谱图和层析条件都得到了验证。此外，WC3MRC将通过精确的质谱学使用非定向代谢方法来进行发现驱动的项目，包括化合物鉴定。先进核心实验室中使用的方法将被机械化，并转移到中央服务核心，供充值服务使用。将开发新的服务和工具，从同位素标记的流量分析到图像引导的代谢组学，这些服务和工具将使用成像设施与已建立的客户联系起来。将通过对HumanCyc酶的精选和使用Inchi结构识别符来改进途径注释，这些结构识别符将用于构建完整的代谢网络，这些网络随后将用于途径超过浓缩的统计分析。当地和地区的科学家将通过年度、竞争性试点和可行性奖获得聘用。参与和奖励标准已经制定，并将由反恐委员会执行。培训计划将在技术和医学层面上教育下一代代谢组学科学家。