Center for Lung Development Imaging and Omics

描述(由申请人提供)：美国是早产婴儿最多的国家之一，相应地，第一天的婴儿死亡率也很高。扩大我们对肺泡形成的理解是促进早产儿正常肺形成的关键一步。然而，这仍然是一个悬而未决的挑战，因为以前还没有对儿童晚期和早期正常肺发育的分子成分进行系统研究。为此，NHLBI正在建立四个研究中心，以开发发育中的肺的分子图谱(LongMAP)。为了响应这一号召，我们组建了一个具有必要经验的研究团队，成功地建立了基于空间成像和细胞特异性组学技术的小鼠和人类肺的分子图谱。在像肺这样的器官中，空间、解剖和功能之间的关系是基本的。因此，我们的方法将包括高空间分辨率的成像技术，以及细胞特异性组学。这些互补数据收集方法的未来相关性将有助于在整个发育肺中建立特定于细胞的空间信息学。具体地说，我们将通过以下目标实现我们的目标：(1)为发育中的小鼠和人类肺的分子图谱提供空间成像；(2)为发育中的小鼠和人类肺的分子图谱提供特定细胞类型的组学；(3)管理数据和元数据，以促进合作和数据集成。总体而言，这些目标将创建第一个时空分子图谱 与其他LUNGMAP中心合作，将提供一系列前所未有的关于小鼠和人类健康发育肺的信息。在PNNL的一个实验室开发和提供的新的成像方法和一套集成的泛组学能力(即蛋白质组学、脂类组学、代谢组学和基于活性的蛋白质图谱)代表了研究中心的独特优势。(摘要结束)

DESCRIPTION (provided by applicant): The United States is one of the top nations for prematurely born infants and has a correspondingly high day- one infant mortality rate. Expanding our understanding of alveologenesis is a critical step toward promoting proper lung formation in preterm infants. This, however, remains an unsolved challenge as no systematic study of the molecular components of normal lung development during late term and early childhood has previously been conducted. For this reason, NHLBI is establishing four research centers to develop a molecular atlas of the developing lung (LungMAP). In response to this call, we have assembled a research team with the necessary experience to successfully establish a molecular atlas of the mouse and human lung based on spatial imaging and cell-specific -omic technologies. Within organs such as the lung, the relationship between space, anatomy, and function is fundamental. Therefore, our approach will include imaging techniques with high spatial resolution, as well as cell-specific -omics. The future correlation of these complementary data collection methods will facilitate the establishment of cell-specific spatial informatics acros the developing lung. Specifically, we will accomplish our goal of an integrated molecular atlas of lung development through the following aims: (1) Spatial imaging for a molecular atlas of the developing mouse and human lung (2) Cell type- specific omics for a molecular atlas of the developing mouse and human lung (3) Manage data and metadata to facilitate collaboration and data integration. Overall, these aims will create the first spatial-temporal molecular atlas of the mammalian lung during alveologenesis, which in coordination with the other LungMAP centers will provide an unprecedented array of information about the healthy developing lungs in mouse and human. The novel imaging approaches and the suite of integrated pan-omics capabilities (i.e. proteomics, lipidomics, metabolomics and activity-based protein profiling) developed and available in a single laboratory at PNNL represents a unique strength of the Research Center. (End of Abstract)