Center for Lung Development Imaging and Omics

描述（申请人提供）：美国是早产儿最多的国家之一，第一天的婴儿死亡率也相应较高。扩大我们对肺泡发生的理解是促进早产儿正确肺形成的关键一步。然而，这仍然是一个未解决的挑战，因为以前没有对晚期和早期儿童正常肺发育的分子组分进行系统研究。为此，NHLBI正在建立四个研究中心，以开发发育中肺的分子图谱（LungMAP）。为了响应这一号召，我们组建了一个具有必要经验的研究团队，成功地建立了基于空间成像和细胞特异性组学技术的小鼠和人肺的分子图谱。在肺等器官内，空间、解剖结构和功能之间的关系是基本的。因此，我们的方法将包括具有高空间分辨率的成像技术以及细胞特异性组学。这些互补的数据收集方法的未来相关性将有助于建立细胞特异性的空间信息学在整个发育中的肺。具体而言，我们将通过以下目标实现肺发育的综合分子图谱的目标：（1）发育中的小鼠和人肺的分子图谱的空间成像（2）发育中的小鼠和人肺的分子图谱的细胞类型特异性组学（3）管理数据和元数据以促进协作和数据集成。总的来说，这些目标将创建第一个时空分子图谱， 在肺泡发生期间，该中心将与其他LungMAP中心协调，提供关于小鼠和人类健康发育的肺的前所未有的信息。PNNL的一个实验室开发并提供的新型成像方法和一系列集成泛组学功能（即蛋白质组学、脂质组学、代谢组学和基于活性的蛋白质分析）代表了研究中心的独特优势。(End摘要）

DESCRIPTION (provided by applicant): The United States is one of the top nations for prematurely born infants and has a correspondingly high day- one infant mortality rate. Expanding our understanding of alveologenesis is a critical step toward promoting proper lung formation in preterm infants. This, however, remains an unsolved challenge as no systematic study of the molecular components of normal lung development during late term and early childhood has previously been conducted. For this reason, NHLBI is establishing four research centers to develop a molecular atlas of the developing lung (LungMAP). In response to this call, we have assembled a research team with the necessary experience to successfully establish a molecular atlas of the mouse and human lung based on spatial imaging and cell-specific -omic technologies. Within organs such as the lung, the relationship between space, anatomy, and function is fundamental. Therefore, our approach will include imaging techniques with high spatial resolution, as well as cell-specific -omics. The future correlation of these complementary data collection methods will facilitate the establishment of cell-specific spatial informatics acros the developing lung. Specifically, we will accomplish our goal of an integrated molecular atlas of lung development through the following aims: (1) Spatial imaging for a molecular atlas of the developing mouse and human lung (2) Cell type- specific omics for a molecular atlas of the developing mouse and human lung (3) Manage data and metadata to facilitate collaboration and data integration. Overall, these aims will create the first spatial-temporal molecular atlas of the mammalian lung during alveologenesis, which in coordination with the other LungMAP centers will provide an unprecedented array of information about the healthy developing lungs in mouse and human. The novel imaging approaches and the suite of integrated pan-omics capabilities (i.e. proteomics, lipidomics, metabolomics and activity-based protein profiling) developed and available in a single laboratory at PNNL represents a unique strength of the Research Center. (End of Abstract)