Center for Lung Development Imaging and Omics

肺发育成像和组学中心

• 批准号: 9493536
• 负责人: Charles K Ansong
• 金额: $ 90.37万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-15 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9493536
• 关键词: 2 year old; Alveolar; Anatomy; Atlases; Biology; Birth; Cell Separation; Cells; Collaborations; Communication; Communities; Core Facility; Data; Data Analyses; Data Collection; Data Coordinating Center; Development; Embryo; Epithelial; Future; Gene Expression; Genes; Goals; Health; Histology; Human; Image; Imaging Techniques; In Situ Hybridization; Infant Mortality; Informatics; Laboratories; Learning; Lipids; Lung; Magnetism; Maps; Medicine; Metadata; Methodology; Methods; Molecular; Molecular Profiling; Mus; National Heart, Lung, and Blood Institute; Organ; Outcome; Population; Premature Infant; Procedures; Process; Proteome; Proteomics; Protocols documentation; Publishing; Quality Control; RNA; Research; Research Project Grants; Resolution; Resources; Sampling; Spatial Distribution; Specific qualifier value; Spectrometry, Mass, Electrospray Ionization; Structure of parenchyma of lung; Techniques; Technology; Time; Tissue Sample; United States; Universities; Update; Washington; activity-based protein profiling; cell type; college; data integration; data management; early childhood; experience; human tissue; imaging approach; interest; lung basal segment; lung development; metabolome; metabolomics; nano; novel; phosphoproteomics; prevent; protein metabolite; response

DESCRIPTION (provided by applicant): The United States is one of the top nations for prematurely born infants and has a correspondingly high day- one infant mortality rate. Expanding our understanding of alveologenesis is a critical step toward promoting proper lung formation in preterm infants. This, however, remains an unsolved challenge as no systematic study of the molecular components of normal lung development during late term and early childhood has previously been conducted. For this reason, NHLBI is establishing four research centers to develop a molecular atlas of the developing lung (LungMAP). In response to this call, we have assembled a research team with the necessary experience to successfully establish a molecular atlas of the mouse and human lung based on spatial imaging and cell-specific -omic technologies. Within organs such as the lung, the relationship between space, anatomy, and function is fundamental. Therefore, our approach will include imaging techniques with high spatial resolution, as well as cell-specific -omics. The future correlation of these complementary data collection methods will facilitate the establishment of cell-specific spatial informatics acros the developing lung. Specifically, we will accomplish our goal of an integrated molecular atlas of lung development through the following aims: (1) Spatial imaging for a molecular atlas of the developing mouse and human lung (2) Cell type- specific omics for a molecular atlas of the developing mouse and human lung (3) Manage data and metadata to facilitate collaboration and data integration. Overall, these aims will create the first spatial-temporal molecular atlas of the mammalian lung during alveologenesis, which in coordination with the other LungMAP centers will provide an unprecedented array of information about the healthy developing lungs in mouse and human. The novel imaging approaches and the suite of integrated pan-omics capabilities (i.e. proteomics, lipidomics, metabolomics and activity-based protein profiling) developed and available in a single laboratory at PNNL represents a unique strength of the Research Center. (End of Abstract)

描述（申请人提供）：美国是早产儿最多的国家之一，第一天的婴儿死亡率也相应较高。扩大我们对肺泡发生的理解是促进早产儿正确肺形成的关键一步。然而，这仍然是一个未解决的挑战，因为以前没有对晚期和早期儿童正常肺发育的分子组分进行系统研究。为此，NHLBI正在建立四个研究中心，以开发发育中肺的分子图谱（LungMAP）。为了响应这一号召，我们组建了一个具有必要经验的研究团队，成功地建立了基于空间成像和细胞特异性组学技术的小鼠和人肺的分子图谱。在肺等器官内，空间、解剖结构和功能之间的关系是基本的。因此，我们的方法将包括具有高空间分辨率的成像技术以及细胞特异性组学。这些互补的数据收集方法的未来相关性将有助于建立细胞特异性的空间信息学在整个发育中的肺。具体而言，我们将通过以下目标实现肺发育的综合分子图谱的目标：（1）发育中的小鼠和人肺的分子图谱的空间成像（2）发育中的小鼠和人肺的分子图谱的细胞类型特异性组学（3）管理数据和元数据以促进协作和数据集成。总的来说，这些目标将创建第一个时空分子图谱， 在肺泡发生期间，该中心将与其他LungMAP中心协调，提供关于小鼠和人类健康发育的肺的前所未有的信息。PNNL的一个实验室开发和提供的新型成像方法和一套集成的泛组学功能（即蛋白质组学，脂质组学，代谢组学和基于活性的蛋白质分析）代表了研究中心的独特优势。(End摘要）

项目成果

Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy.

使用纳米喷雾解动电动质谱法与剪切力显微镜耦合到高分辨率组织成像。

• DOI: 10.1007/s13361-017-1750-8
• 发表时间: 2018-03
• 期刊: Journal of the American Society for Mass Spectrometry
• 影响因子: 3.2
• 作者: Nguyen SN;Sontag RL;Carson JP;Corley RA;Ansong C;Laskin J
• 通讯作者: Laskin J

Lipid Coverage in Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Mouse Lung Tissues.

小鼠肺组织纳喷雾解吸电喷雾电离质谱成像中的脂质覆盖。

• DOI: 10.1021/acs.analchem.9b02045
• 发表时间: 2019-09-17
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Nguyen, Son N.;Kyle, Jennifer E.;Dautel, Sydney E.;Sontag, Ryan;Luders, Teresa;Corley, Richard;Ansong, Charles;Carson, James;Laskin, Julia
• 通讯作者: Laskin, Julia

Lipidomics reveals dramatic lipid compositional changes in the maturing postnatal lung.

• DOI: 10.1038/srep40555
• 发表时间: 2017-02-01
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Dautel SE;Kyle JE;Clair G;Sontag RL;Weitz KK;Shukla AK;Nguyen SN;Kim YM;Zink EM;Luders T;Frevert CW;Gharib SA;Laskin J;Carson JP;Metz TO;Corley RA;Ansong C
• 通讯作者: Ansong C