Validation of methods for detection of Campylobacter in veterinary patients

项目摘要 弯曲杆菌感染是美国人类食源性疾病的主要原因。 虽然人类弯曲杆菌病通常与食用 受污染的食物，宠物可能是另一个感染源。狗和猫通常 被认为是人类感染的来源，但弯曲杆菌携带是公认的， 这些宠物。狗和猫感染弯曲杆菌的危险因素包括食用 生肉饮食和受污染的食物，如未煮熟的牛骨。人们会被暴露 在处理受污染的宠物食品和治疗或受感染宠物的粪便时， 尽管认识到这些病例和狗和猫的弯曲杆菌病的潜在风险， 在这些菌种中不常规进行弯曲杆菌培养。我们建议验证 诊断培养和分子方法检测狗和猫的弯曲杆菌脱落 因为1）弯曲杆菌感染在狗和猫中是众所周知的; 2）狗和猫生活在 与人，特别是儿童密切相关; 3）犬科动物和猫科动物脱落 弯曲杆菌呈是公认的，与人类感染有关; 4）狗和猫 没有进行弯曲杆菌脱落的常规筛查，尽管认识到 弯曲杆菌感染的狗和猫;和5）缺乏标准化的方法， 弯曲杆菌检测使得不同地理区域的研究难以进行比较 不同的实验室。我们将验证培养和分子方法， 方法在3个独立的设盲实验室中使用弯曲杆菌加标供试品， 最后在患腹泻的狗的粪便样本上测试这些方法。此外，本发明还 4个合作机构之间的测试验证和样本交换的协作努力 实验室将加强这些Vet-LIRN实验室之间的联系， 在实际紧急事件相关事件期间进行无缝交互。

PROJECT SUMMARY Campylobacter infection is a leading cause of human foodborne illness in the United States. Although human campylobacteriosis is usually associated with the consumption of contaminated food, pets can be another source of infection. Dogs and cats are not commonly thought of as a source for human infection, but Campylobacter carriage is well-recognized in these pets. Risk factors for Campylobacter infection in dogs and cats include consumption of raw meat diets and contaminated treats such as uncooked beef bones. People can be exposed to Campylobacter when handling contaminated pet food and treats or feces from infected pets. Despite recognition of these cases and the potential risk of campylobacteriosis in dogs and cats, Campylobacter culture is not routinely performed in these species. We propose to validate diagnostic culture and molecular methods to detect Campylobacter shedding in dogs and cats because 1) Campylobacter infection is well-recognized in dogs and cats; 2) dogs and cats live in close association with people, particularly children; 3) canine and feline shedding of Campylobacter spp. is well-recognized and associated with human infection; 4) dogs and cats are not routinely screened for Campylobacter shedding despite recognition of the risk of Campylobacter infection in dogs and cats; and 5) the absence of standardized methods for Campylobacter detection make it difficult to compare studies from different geographic areas and different laboratories. We will validate both culture and molecular methods and test these methods in 3 independent, blinded laboratories with Campylobacter spiked test samples and ultimately test the methods on fecal samples from canine patients with diarrhea. Additionally, the collaborative effort of test validation and sample exchange between the 4 collaborating laboratories will strengthen the ties between these Vet-LIRN laboratories thereby promoting seamless interactions during actual emergency related events.