Enhancement of molecular capabilities of the TAMU VMTH CML

项目摘要 在美国，食源性疾病每年都会导致显著的发病率和死亡率。 沙门氏菌、弯曲杆菌和李斯特菌都能引起人和动物的感染。虽然 人类食源性疾病通常与食用受污染的食物、宠物 可以是细菌病原体的直接和间接来源。美国食品和药物管理局 (FDA)兽医实验室综合反应网络（Vet-LIRN） 实验室合作协议计划旨在促进人类和动物健康 通过提供科学信息和建立实验室能力， 应对疫情调查并调查动物饲料问题， 毒品检测疫情需要能够从地理上不同的地方收集样本 地区得克萨斯州有2500万人口，人口仅次于加州， 马和牛的数量居全国之首因此，重要的是要了解 德克萨斯州内动物和人之间食源性疾病的动态。的 德克萨斯A&M大学兽医教学医院临床微生物学实验室 目前是FDA Vet-LIRN实验室。实验室的成员帮助 开发用于检测沙门氏菌的Vet-LIRN方法，包括分子检测， 空肠弯曲菌，参与能力验证，并采集和分析样品 作为Vet-LIRN调查的一部分。本提案的目的是验证C。空肠回 PCR检测牛粪便，用新的实时PCR设备取代老化的实时PCR设备 与其他兽医诊断实验室所用设备兼容的设备， 从食源性病原体中提取核酸。该基质的方法验证 将允许快速，有效地检测动物样本中的病原体，以支持FDA Vet-LIRN的使命是通过迅速识别疫情来保护人类和动物健康 和检测与疫情有关的样本。

PROJECT SUMMARY Foodborne illness causes significant morbidity and mortality each year in the United States. Salmonella, Campylobacter, and Listeria, all cause infections in people and animals. Although human foodborne illness is usually associated with the consumption of contaminated food, pets can be direct and indirect sources of bacterial pathogens. The Food and Drug Administration (FDA) Veterinary Laboratory Integrated Response Network (Vet-LIRN) Veterinary Diagnostic Laboratory Cooperative Agreement Program is designed to promote human and animal health by providing scientific information and building laboratory capacity for routine and emergency response for investigation of outbreaks and to investigate problems with animal feeds and drugs. Detection of outbreaks requires the ability to collect samples from geographically diverse areas. Texas is home to 25 million people, second only to California in human population, and leads the nation in horse and cattle populations. It is therefore important to understand the dynamics of foodborne diseases among animals and people within the State of Texas. The Texas A&M University Veterinary Medical Teaching Hospital Clinical Microbiology Laboratory currently serves as a FDA Vet-LIRN Laboratory. Members of the laboratory have helped to develop Vet-LIRN methods for detection, including molecular detection, of Salmonella and Campylobacter jejuni, participated in proficiency testing, and collecting and analyzing samples as part of Vet-LIRN investigations. The objective of this proposal is to validate the C. jejuni gyrA PCR for testing bovine feces and to replace aging real-time PCR equipment with new equipment compatible with that used in other veterinary diagnostic laboratories for detection of nucleic acid from foodborne pathogens with equipment. Validation of the method for this matrix will allow rapid, efficient detection of pathogens in samples from animals to support the FDA Vet-LIRN mission of protecting human and animal health by prompt recognition of outbreaks and testing of samples associated with outbreaks.